Genetic Profiling to Determine Potential Origins of Boll Weevils (Coleoptera: Curculionidae) Captured in a Texas Eradication Zone: Endemicity, Immigration, or Sabotage?

Kim, Kyung Seok; Sappington, Thomas W.; Allen, Charles T.

doi:10.1603/0022-0493-101.6.1729

Cited by 15 publications

(23 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2). Each ampliÞcation product generated with the microsatellite primers was consistent with its expected size range as reported from a natural boll weevil population (Kim and Sappington 2004b) and as encountered in subsequent surveys involving 22 populations over a large geographic area including eight U.S. states, and northern Mexico Kim et al , 2008. There was no apparent Fig.…”

Section: Resultssupporting

confidence: 79%

“…2) All three microsatellite loci will amplify from separate body parts of dismembered weevils. We know from previous studies involving whole body samples of Ͼ700 individual A. grandis grandis from 22 populations in the United States and Mexico that these three loci are present in this species and always amplify under appropriate PCR conditions Sappington 2004b, 2006;Kim et al , 2008. We had not previously tested these loci in A. g. thurberiae, but we suspected that they would amplify because, as a subspecies of A. g. grandis, they are evolutionarily closely related.…”

Section: Methodsmentioning

confidence: 99%

“…The Þrst hypothesis can be accepted if the three microsatellite loci, which are present in boll weevil Sappington 2004b, 2006;Kim et al , 2008, are not present in pepper weevil, cranberry weevil, and pecan weevil. Acceptance of this hypothesis would indicate that this set of markers can be used to diagnose an individual as either a boll weevil, or not a boll weevil.…”

Section: Methodsmentioning

confidence: 99%

“…The ability of boll weevil adults to disperse hundreds of kilometers is well-documented (Guerra 1988;Spurgeon et al 1997;Sappington 2004a,b, 2006;Westbrook et al 2007), and both natural dispersal by ßight (Culin et al 1990, Westbrook et al 2007) and human-mediated transport (Sappington et al 2004Kim et al 2008) can reintroduce boll weevils to an eradication zone. The threat of reintroduction is a constant concern because of the expense involved in eliminating a colonizing population that successfully establishes , Westbrook et al 2007, Kiser and Catanach 2008.…”

mentioning

confidence: 99%

“…The threat of reintroduction is a constant concern because of the expense involved in eliminating a colonizing population that successfully establishes , Westbrook et al 2007, Kiser and Catanach 2008. A population genetics approach using neutral molecular DNA markers has provided important clues to the origin of boll weevils captured unexpectedly in or near eradication zones (Kim et al , 2008. In most cases the response is swift, massive, disruptive, and expensive to the program and to the affected growers.…”

mentioning

confidence: 99%

See 4 more Smart Citations

Molecular Diagnostic for Boll Weevil (Coleoptera: Curculionidae) Based on Amplification of Three Species-Specific Microsatellites

Kim¹,

Szendrei²,

Rodriguez‐Saona³

et al. 2009

Self Cite

View full text Add to dashboard Cite

Section: Resultssupporting

confidence: 79%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Molecular Diagnostic for Boll Weevil (Coleoptera: Curculionidae) Based on Amplification of Three Species-Specific Microsatellites

Kim¹,

Szendrei²,

Rodriguez‐Saona³

et al. 2009

Self Cite

View full text Add to dashboard Cite

Source tracing of Anthonomus grandis captured in areas of the USA where the species had previously been eradicated

et al. 2023

View full text Add to dashboard Cite

Anthonomus grandis Boheman (Coleoptera: Curculionidae) is comprised of two subspecies: the boll weevil (A. g. grandis) and the thurberia weevil (A. g. thurberiae). The thurberia weevil is generally regarded as a non-pest variant of the species, whereas the boll weevil is an important economic pest of commercial cotton. In the USA, a nationwide eradication program has been largely successful against the boll weevil, but reintroductions of eradicated zones remain a threat. In 2015, A. grandis was trapped in the functionally eradicated Winter Garden growing region in Texas, where it had not been found since 2012. Weevils established in the area and triggered a multi-year, multi-million-dollar mitigation effort. In 2017, the species was trapped in Hidalgo County, New Mexico, another area where it had been previously eradicated. In this study, we obtained specimens from both trapping events and used double digest restriction site-associated DNA sequencing to obtain genetic data in homology with previously published A. grandis population genetic data. We conducted source tracing to match the captured weevils to previously described populations to determine a likely source population. We also compared the accuracy of the approach to other diagnostic methods currently used by the United States Department of Agriculture. The results suggested that a small founder population of weevils dispersed from the Lower Rio Grande Valley population in southern Texas and northern Tamaulipas and established in the Winter Garden. In Hidalgo Co., NM, the weevils were found to be genetically indistinct from non-pest populations of thurberia weevil associated with Arizona wild cotton (Gossypium thurberi). Thurberia weevils in Arizona remain unlikely to threaten commercial growers. Improved rapid diagnostic tools may be desirable to enable better assessment of trap captures and allow for a reduction in the application of mitigation efforts when the non-pest variant is identified.

show abstract

Isolation and characterization of eight microsatellite loci from Lycorma delicatula (White) (Hemiptera: Fulgoridae) for population genetic analysis in Korea

2011

View full text Add to dashboard Cite

Lycorma delicatula (White) is native to China but is becoming an important insect pest in Korea. Polymorphic DNA markers like microsatellites are widely used for characterizing dispersal patterns and capacity of invasive insect pests which can contribute to designing effective management of the species. To facilitate such population genetic studies of L. delicatula in Korea, we isolated and characterized eight microsatellite loci for L. delicatula using a hybridization-biotin enrichment method. We further used these novel microsatellite loci to determine population genetic parameters for 33 L. delicatula specimens collected from Cheonan, South Korea where outbreaks of this species were first reported in Korea. The number of alleles per locus ranged from three to ten, with an average of 6.25. The mean expected (H (E)) and observed heterozygosities (H (O)) were 0.575 and 0.626, respectively. The eight loci showed no deviation from Hardy-Weinberg equilibrium according to the adjusted significance threshold (P = 0.00625), and there was no linkage disequilibrium between each pair of these eight markers. Bayesian cluster analysis using the program structure revealed no evidence of genetic structuring in L. delicatula samples from Cheonan. These new microsatellite markers will be widely applicable to future ecological genetic studies of L. delicatula, including assessment of the level of gene flow and genetic connectivity among populations that are necessary for effective management and monitoring of the species.

show abstract

Genetic Profiling to Determine Potential Origins of Boll Weevils (Coleoptera: Curculionidae) Captured in a Texas Eradication Zone: Endemicity, Immigration, or Sabotage?

Cited by 15 publications

References 16 publications

Molecular Diagnostic for Boll Weevil (Coleoptera: Curculionidae) Based on Amplification of Three Species-Specific Microsatellites

Molecular Diagnostic for Boll Weevil (Coleoptera: Curculionidae) Based on Amplification of Three Species-Specific Microsatellites

Source tracing of Anthonomus grandis captured in areas of the USA where the species had previously been eradicated

Isolation and characterization of eight microsatellite loci from Lycorma delicatula (White) (Hemiptera: Fulgoridae) for population genetic analysis in Korea

Contact Info

Product

Resources

About