Genetic Profiles Affect the Biological Effects of Serine on Gastric Cancer Cells

Abstract

A high serine content in body fluid was identified in a portion of patients with gastric cancer, but its biological significance was not clear. Here, we investigated the biological effect of serine on gastric cancer cells. Serine was added into the culture medium of MGC803 and HGC27 cancer cells, and its influence on multiple biological functions, such as cell growth, migration and invasion, and drug resistance was analyzed. We examined the global transcriptomic profiles in these cultured cells with high serine content. Both MGC803 and HGC27 cell lines were originated from male patients, however, their basal gene expression patterns were very different. The finding of cell differentiationassociated genes, ALPI, KRT18, TM4SF1, KRT81, A2M, MT1E, MUC16, BASP1, TUSC3, and PRSS21 in MGC803 cells suggested that this cell line was more poorly differentiated, compared to HGC27 cell line. When the serine concentration was increased to 150mg/ml in medium, the response of these two gastric cancer cell lines was different, particularly on cell growth, cell migration, and invasion and 5-FU resistance. In animal experiment, administration of high concentration of serine promoted cancer cell metastasis to local lymph node. Taken together, we characterized the basal gene expressing profiles of MGC803 and HGC27. The HGC27 cells were more differentiated than MGC803 cells. MGC803 cells were more sensitive to the change of serine content. Our results suggested that the responsiveness of cancer cells to microenvironmental change is associated with their genetic background.

Prediction and verification of the prognostic biomarker SLC2A2 and its association with immune infiltration in gastric cancer

“…Among them, HGC-27 cells were derived from relatively differentiated GC tissues, NCI-N87 cells were isolated from male patients with GC complicated with liver metastasis and AGS cells were derived from an untreated primary GC excision fragment. All three types of GC cells had different degrees of malignant transformation potential ( 21 – 23 ). SLC2A2 expression was the highest in NCI-N87 cells and significantly differed from that in GES-1 (P=0.0174), AGS (P=0.026) and HGC-27 cells (P=0.0009), indicating that the expression of SLC2A2 was related to the degree of malignancy.…”

Metabolite biomarker discovery for human gastric cancer using dried blood spot mass spectrometry metabolomic approach

“…As a non-essential amino acid, Asp is the basic substrate for the synthesis of pyrimidine and purine nucleosides. Furthermore, Ser is also involved in the synthesis of purine nucleotides via Gly, and it can influence cell growth and invasion of cancer cells 23 . The increased uptake rates of Asp and Ser imply that these amino acids are needed in fueling nucleoside biosynthesis for tumor proliferation 24 .…”

Editorial: Chemo-Radiation-Resistance in Cancer Therapy

“…A high serine content in body fluid was identified in a portion of patients with gastric cancer, but its biological significance, such as cell growth, migration and invasion, and drug resistance, was not clearly. Jun Li et al ( Li et al, 2020 ) characterized the basal gene expressing profiles of MGC803 and HGC27. The HGC27 cells were more differentiated than MGC803 cells while MGC803 cells were more sensitive to the change of serine content.…”