Genetic profile of overweight and obese school-age children

The aim of the MAPEC_LIFE (Monitoring Air Pollution Effects on Children for Supporting Public Health Policy) study is to evaluate the associations between the concentrations of air pollutants and early biological effects in children living in five Italian towns (Brescia, Torino, Lecce, Perugia and Pisa) characterised by varying levels of air pollution. This paper presents the results of micronucleus cytome assays performed on the oral mucosa cells of subjects living in Lecce (Puglia, Italy) and their relationship to factors associated with indoor/outdoor exposure and lifestyles. The study was conducted on 6-8-year-old schoolchildren living in Lecce. The micronucleus cytome assay was performed on exfoliated buccal cells collected from the oral mucosa of children using a soft-bristled toothbrush. Micronuclei were evaluated only in normal differentiated cells. Overall, 43.0% of the samples tested were positive, with an average frequency of 0.28 MN/1000 differentiated cells. Data analysis shows positive associations between the frequency of MN in the children's buccal mucosa cells and obesity, heavy traffic and smoking mothers, while outdoor sports seem to have the opposite effect. These data will be integrated with data from the other cities involved in the MAPEC_LIFE study and could be used to build a model for estimating global genotoxic risk.

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“…This positive correlation is confirmed by the literature for both peripheral lymphocytes [34] and BM cells [35] in school-age children.…”

Section: Discussionsupporting

confidence: 80%

Air pollution biological effects in children living in Lecce (Italy) by Buccal micronucleus cytome assay (the MAPEC_LIFE study)

Donno¹,

Grassi²,

Ceretti³

et al. 2016

Int. J. SDP

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“…However, other parameters that could affect the frequency of MN and NAs, such as prediabetes, metabolic syndrome, or obesity, which are also related to inflammatory processes and DNA damage, were not considered [4,15,37,[45][46][47][48][49][50][51].…”

Section: Effects Of Dmmentioning

confidence: 98%

Nuclear abnormalities in buccal mucosa cells of patients with type I and II diabetes treated with folic acid

Gómez‐Meda

Zamora‐Pérez

Muñoz-Magallanes

et al. 2016

Mutation Research/Genetic Toxicology and Environmental Mutagene

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“…In the literature, numerous studies conducted on both adults and children have provided evidence regarding the positive correlation between the frequency of early-effect biomarkers and factors such as obesity [21,22], respiratory diseases [23,24], residence in areas with high traffic levels [25], exposure to passive smoking [26,27], and inhalation or ingestion of toxic compounds formed during cooking with certain methods (grilling, on the griddle, frying, toasting) [28][29][30]. In contrast, a high level of parental education [31,32] and intake of antioxidant substances and vitamins in the diet [19,33,34] could be negatively related to the frequency of MN.…”

Section: Introductionmentioning

confidence: 99%

Micronucleus Frequency in Exfoliated Buccal Cells of Children Living in an Industrialized Area of Apulia (Italy)

Panìco

Grassi

Bagordo

et al. 2020

IJERPH

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Micronuclei (MN) are biomarkers of early biological effect often used for detecting DNA damage in human population exposed to genotoxic agents. The aim of this study was to evaluate the frequency of MN in exfoliated buccal cells of children living in an industrialized (impacted) area compared with that found in children living in a control area without significant anthropogenic impacts. A total of 462 6–8-year-old children (206 in the impacted area, 256 in the control area) attending primary school were enrolled. A questionnaire was administered to the parents of the recruited children to obtain information about personal data, lifestyles, and food habits of their children. Atmospheric particulate fractions were collected near the involved schools to assess the level of environmental exposure of the children. The presence of MN was highlighted in 68.4% of children living in the impacted area with a mean MN frequency of 0.66‰ ± 0.61‰. MN positivity and frequency were significantly lower in the control area (37.1% and 0.27‰ ± 0.43‰, respectively). The frequency of MN was positively associated with quasi-ultrafine particulate matter (PM0.5), traffic near the home, and consuming barbecued food; while adherence to the Mediterranean diet and practicing sport were negatively associated.

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Genetic profile of overweight and obese school-age children

Cited by 12 publications

References 24 publications

Air pollution biological effects in children living in Lecce (Italy) by Buccal micronucleus cytome assay (the MAPEC_LIFE study)

Air pollution biological effects in children living in Lecce (Italy) by Buccal micronucleus cytome assay (the MAPEC_LIFE study)

Nuclear abnormalities in buccal mucosa cells of patients with type I and II diabetes treated with folic acid

Micronucleus Frequency in Exfoliated Buccal Cells of Children Living in an Industrialized Area of Apulia (Italy)

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