Genetic Predisposition to Bronchopulmonary Pathology in Workers of Harmful and Hazardous Industries: Analysis of Five Polymorphisms of Dna Gene Repair

Andrushchenko, Т. A.; Гончаров, С. В.; Dosenko, Victor E.

doi:10.15407/fz64.04.012

Cited by 6 publications

(5 citation statements)

References 13 publications

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“…хімічних факторів [3,4]. Захворювання органів дихання від впливу промислових аерозолів посідають провідні позиції в структурі професійних захворювань і лишаються проблемою медицини праці, у зв'язку з чим їх профілактика є важливою медичною, соціальною та економічною задачею [5].…”

Section: вступunclassified

Acoustic Analysis of Voice Meas- Ures in Chronic Laryngitis Patients

Kovalchuk¹,

Shydlovska²

2021

Fiziol Zh

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We aimed to analyse voice signals in 40 patients with chronic laryngitis elicited by exposure to chemical factors. We ex- amined 20 people with catarrhal chronic laryngitis (group 1), 20 people with subatrophic chronic laryngitis (group 2) and 15 healthy volunteers as controls. All subjects underwent acoustic examination of the voice signal using the software Praat V 4.2.1. We studied acoustic measures as follows: Jitter, Shimmer and NHR (noise-to-harmonics ratio). The analysis of the obtained data revealed statistically significant differ- ences in the average values of Jitter and Shimmer measures, as well as in the ratio of nonharmonic (noise) and harmonic component in the spectrum ( NHR) in patients with chronic laryngitis (groups 1 and 2) compared with controls. In group 1 (chronic catarrhal laryngitis), the average values of acoustic measures such as Jitter, Shimmer and NHR were as follows: Jitter - 0.92 ± 0.1%, Shimmer - 5.31 ± 0.5%, NHR - 0.078 ± 0.04. In group 2 (subatorophic laryngitis), the average values of acoustic measures were: Jitter - 0.67 ± 0.6%, Shimmer - 6.57 ± 0.7% and NHR - 0.028 ± 0.003. The obtained data indicate a pronounced instability of the voice in frequency and amplitude, a significant proportion of the noise component in the spectrum of the voice signal in the examined patients with chronic laryngitis exposed to chemical factors. The most pronounced alterations were found in patients with catarrhal chronic laryngitis. We conclude that the quantitative values of spectral analysis of the voice signal Jitter, Shimmer, NHR may serve as valuable criteria of the degree of voice impair- ment. This may be helpful in determining the effectiveness of rehabilitation measures.

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Section: вступunclassified

Acoustic Analysis of Voice Meas- Ures in Chronic Laryngitis Patients

Kovalchuk¹,

Shydlovska²

2021

Fiziol Zh

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“…DNA for genotyping was isolated from peripheral blood leukocytes using the Neo-Prep100DNA and NEOGENE (Ukraine) kits in accordance with the manufacturer's instructions. Polymerase chain reaction (PCR) in real time was used to determine genes SNP: XPd (rs13181, rs799793), ercc1 (rs11615), Xrcc3 (rs861539), Xrcc1 (rs25487), ATM (rs664677), Xrcc7 (rs7003908) and MLh1 (rs1799977) with the use of 7500 Fast Real-Time PCR System Appliter (Applied Biosystems, USA) and TaqMan Assays [6,7].…”

Section: Characteristics Of the Research Respondentsmentioning

confidence: 99%

“…Previously we studied the frequency of allelic variants of DNA repair genes polymorphisms in two independent groups of workers of hazardous and harmful industries in Ukraine [6]. To confirm the prognostic importance of the distribution of DNA repair genes allelic polymorphisms, it is necessary to standardize the results obtained and to prove that in workers of different harmful industries the frequencies of minor and dominant homozygotes and heterozygotes deviate significantly from the theoretically expected values.…”

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confidence: 99%

“…Double-strand break repair (DSBR) errors cause mutations and chromosome rearrangements that induce genome instability and carcinogenesis [6,7]. There are two mechanisms of DSBR repair: nonhomologous end joining (NHEJ) and homologous recombination (HR) [6,8]. NHEJ provides the ligation of DNA ends with minimal enzymatic treatment on the site of their combination.…”

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confidence: 99%

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Allelic polymorphisms of DNA repairing genes as markers of resistance to asbestos-containing aerosols

Andrushchenko¹,

Гончаров²,

Dosenko³

et al. 2019

Ukr.Biochem.J

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We studied the frequency distribution of dNA repairing genes allelic polymorphisms in the occupational group of asbestos-cement plants workers (n = 95). The aim of the work was to determine the probable molecular genetic markers of resistance to the development of bronchopulmonary pathology under the action of chrysotile-asbestos-containing dust. In 46 workers with chronic forms of bronchopulmonary pathology and 49 workers of the same professions without chronic diseases of the respiratory system, allelic polymorphisms XPd (rs13181, rs799793), ercc1 (rs11615), Xrcc3 (rs861539), Xrcc1 (rs25487), ATM (rs664677), Xrcc7 (rs7003908) and MLh1 (rs1799977) were determined using the real-time polymerase chain reaction.

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“…In the structure of harmful and dangerous occupational factors in main branches of modern industry, there are those that can lead to disorders in the Double-strand break repair (DSBR) [1,2] and cause the development of chronic Broncho-pulmonary pathology (BPP). Industrial aerosols are represented by a large variety of chemical composition, most often found in them: compounds of silicon, calcium, and carbon; oxides of iron, magnesium, manga nese; radioactive elements -strontium, polonium, titanium, lead, potassium and others.…”

Section: Introductionmentioning

confidence: 99%

Correlations between polymorphisms of double-strand break DNA repair genes and risk of bronchopulmonary pathology development in hazardous industries workers

Andrushchenko¹,

Гончаров²,

Dosenko³

2018

Fiziol Zh

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The study in the group of workers of asbestos-cement plants and miners of allelic variants of XRCC7 (rs7003908) and ATM (rs664677) polymorphisms encoding the double-strand break repair DNA (n=215). These polymorphisms are recognized markers of cancer of various types and localizations, as well as markers of radio sensitivity/resistance in the influence of ionizing radiation. The respondents of the research group were workers of asbestos-cement plants and coal miners in the anamnesis in with bronchopulmonary pathology, the control group consisted of workers without diseases of the respiratory system. The genotypes of the genes of the the XRCC7 (rs7003908) and ATM (rs664677) were determined in real time by the polymerase chain reaction method. As a result of the study, it was established that the minor genotype ATM•T/T (OR=2.48 95%CI: 1. 16-5.31; χ²=6.61; P<0.01) are associat with a risk of bronchopulmonary pathology. Also, it was found that the dominant allele of ATM•A (OR=0.69; 95% CI: 0.46-1.04), genotypes: dominant homozygotes АТМ•А/А (ОR=0.83; 95%CI: 0.45-1.54), heterozygotes АТМ•А/Т (ОR=0.67; 95%CI: 0.38-1.21) cause resistance to bronchopulmonary pathology in workers with harmful and dangerous working conditions. The analysis of the frequency distribution of the allelic variants of the gene XRCC7 (rs7003908) suggests that there is no association of this polymorphism with the risk of developing respiratory diseases. The obtained results to the importance of polymorphism of the ATM gene (rs664677) for the repair of double-strand DNA ruptures in the development of diseases of the respiratory system in workers in harmful and dangerous industries.

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Genetic Predisposition to Bronchopulmonary Pathology in Workers of Harmful and Hazardous Industries: Analysis of Five Polymorphisms of Dna Gene Repair

Cited by 6 publications

References 13 publications

Acoustic Analysis of Voice Meas- Ures in Chronic Laryngitis Patients

Acoustic Analysis of Voice Meas- Ures in Chronic Laryngitis Patients

Allelic polymorphisms of DNA repairing genes as markers of resistance to asbestos-containing aerosols

Correlations between polymorphisms of double-strand break DNA repair genes and risk of bronchopulmonary pathology development in hazardous industries workers

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