Genetic polymorphisms of methylenetetrahydrofolate reductase C677T and risk of ischemic stroke in a southern Chinese Hakka population

Abstract

Previous studies have shown that methylenetetrahydrofolate reductase (MTHFR) gene to be a genetic risk factor for the susceptibility to ischemic stroke. The aim of this case-control study was to investigate whether the polymorphisms of MTHFR C677T were associated with the susceptibility to ischemic stroke in a southern Chinese Hakka population. In this study, a total of 1967 ischemic stroke patients and 2565 controls of Chinese Hakka ethnicity were recruited. The MTHFR C677T polymorphisms were genotyped by polymerase chain reaction (PCR) amplification and microarray method. The risk of ischemic stroke was estimated by logistic regression analysis. The frequencies of CC, CT, and TT genotypes were 52.67% versus 55.63%, 40.31% versus 38.52%, and 7.02% versus 5.85% in patients with ischemic stroke versus controls, respectively. The frequency of T allele was significantly higher in ischemic stroke patients (27.17%) than in controls (25.11%) (P = .026, odds ratio [OR] 1.113, 95% confidence interval [CI] 1.013–1.223). The homozygous TT genotype in the ischemic stroke patients was associated with increased risk (P = .049, OR 1.132, 95% CI 1.001–1.281) when compared with the controls after adjustment for age and sex. The positive association was only found in dominant model without adjustment for age and sex (P = .047, OR 1.127, 95% CI 1.002–1.268). Also, the carrier status of the MTHFR T allele was identified as an independent risk factor for the development ischemic stroke even after the adjustment for conventional risk factors (P = 0.047, OR 1.109, 95% CI 0.964–1.225). Our results provide evidence that variants of MTHFR C677T gene may influence the risk of developing ischemic stroke in a southern Chinese Hakka population. Further studies are needed to confirm this association, which will promote the development of strategies for prevention and treatment of ischemic stroke in our study population.

Relationship of Methylenetetrahydrofolate Reductase (MTHFR) C677T Variation With Susceptibility of Patients With Ischemic Stroke: A Meta-Analysis

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Occurrence of MTHFR C677T gene polymorphism and its association with atherogenic indices in Mexican women from San Luis Potosi, a preliminary study

“…Earlier scienti c investigations have revealed that the MTHFR C677T gene polymorphism is a genetic risk factor associated with an increased human susceptibility to develop CVDs (Hou et al 2018;Lakkakula 2019;P.-F. Liu et al 2020). Hou et al (2018) completed a case-control study on the association between MTHFR C677T polymorphism and the predisposition to ischemic stroke in a Southern Chinese Hakka population (n=4532).…”

“…Earlier scienti c investigations have revealed that the MTHFR C677T gene polymorphism is a genetic risk factor associated with an increased human susceptibility to develop CVDs (Hou et al 2018;Lakkakula 2019;P.-F. Liu et al 2020). Hou et al (2018) completed a case-control study on the association between MTHFR C677T polymorphism and the predisposition to ischemic stroke in a Southern Chinese Hakka population (n=4532). The results showed an expanded risk of ischemic stroke in the homozygous polymorphic TT genotype group compared to the homozygous CC genotype and heterozygous CT genotype groups, even after adjusting for traditional risk elements (Hou et al 2018).…”

Methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism rather than homocysteine increase the risk of ischemic stroke-associated executive dysfunction

“…13 Previous studies showed that the MTHFR C677T gene polymorphism among ischemic stroke patients was relatively high, including in the Indonesian population, ranging from 13.9-40.3% for the CT genotype and 3.2-7.0% for the TT genotype. [14][15][16] In general, the variation in the prevalence of these gene polymorphisms is strongly influenced by ethnicity. This gene polymorphism will decrease the MTHFR enzyme activity at 37°C or higher and increase blood homocysteine levels.…”