Genetic Polymorphisms of ADRB2 and IL10 May Be Associated with the Risk of IgE Sensitization to Digestive Powders in Exposed Medical Personnel

Abstract

Background: It has been reported that pancreatic extracts may induce IgE-mediated respiratory allergy in medical personnel. The aim of the study was to identify genetic factors associated with IgE sensitization to digestive powders containing pancreatic extract. Methods: This case-control study was performed on 153 subjects routinely exposed to digestive powder and on 123 nonexposed controls working in Ajou University Hospital. Skin prick testing was performed using 4 commonly used digestive powders and α-amylase. Serum specific IgE levels were measured by ELISA. Three single nucleotide polymorphisms, ADRB2 46A>G, IL10 –1082A>G and IL4 –589T>C, were genotyped using the single base extension method. Results: The positive rate of serum specific IgE to digestive powder was significantly higher in the 41 (26.8%) exposed personnel with work-related respiratory symptoms than in controls (24.4 vs. 5.4%, p = 0.012). Thirty-nine (25.5%) of the 153 exposed personnel were found to have an allergy to digestive powder, as determined by a positive skin prick test and/or a high serum specific IgE level to digestive powder. The ADRB2 46A>G and IL10 –1082A>G polymorphisms were found to be significantly associated with the development of an allergy to digestive powder in exposed medical personnel by multiple logistic regression analysis after controlling for age, atopy and job type (pharmacist or nurse; p = 0.035 and p = 0.027, respectively). Conclusion: These results suggest that the genetic polymorphisms ADRB2 46A>G and IL10 –1082A>G are genetic factors that increase IgE sensitization to pancreatic extracts in medical personnel occupationally exposed to digestive powders.

Identifying Genetic Susceptibility to Sensitization to Cephalosporins in Health Care Workers

“…Digestive enzymes (1) and antibiotics (2, 3) are the main drugs that induce occupational allergies in Korea. In recent years, cephalosporins have become the most commonly prescribed antibiotics in most hospitals.…”