Genetic polymorphisms of 26 Y-STR loci in the Mongolian minority from Horqin district, China

Abstract

To study the population data of Y chromosome STR (Y-STRs) of the Mongolian minority population residing in the Horqin district, we analyzed haplotypes of 26 Y-STRs (DYS19, DYS385a/b, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS481, DYS533, DYS549, DYS570, DYS576, DYS635, DYS643, DYS388, DYS449, DYS460, and YGATAH4) in 298 unrelated Chinese Mongolian individuals using the commercially available Goldeneye® DNA ID 26Y system. We also investigated blood stains, saliva spots, semen spots, hair follicles, fingernails, and sweat latent fingerprints from ten healthy males for testing the efficiency of direct amplification of this new Y-STRs system. The calculated average gene diversity values of the Mongolian population ranged from 0.3024 to 0.9510 for the DYS389I and DYS385a/b loci, respectively. The discriminatory capacity was 92.95 % with 277 observed haplotypes using 23 Y-STR loci (DYS19, DYS385a/b, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS481, DYS533, DYS549, DYS570, DYS576, DYS635, DYS643, and YGATAH4). By adding three more Y-STRs (DYS388, DYS449, and DYS460) to the 26Y system, the discriminatory capacity was increased to 94.63 % with a total of 282 observed haplotypes. Population relationships were calculated and compared with seven populations available from the Y chromosome haplotype reference database and data from ten Asian populations published previously. The Mongolian minority population residing in Horqin district is significantly different from other populations. Our results indicated that these 26 Y-STRs were highly genetically polymorphic in the Mongolian group and this contributes greatly to existing Chinese ethnic genetic information. As a result of direct amplification, we have obtained full profile from all blood stains, saliva spots, hair follicles, and fingernails; six semen spots; and one sweat latent fingerprint. It revealed that the 26 Y-STR system was a valuable tool for male sex analysis in forensic field and the kit was highly adaptive to direct amplification of various samples including blood stain, saliva spot, hair follicle, and fingernail.

RETRACTED: Forensic characteristics and genetic affinity analyses of Xinjiang Mongolian group using a novel six fluorescent dye‐labeled typing system including 41 Y‐STRs and 3 Y‐InDels

“…Up to the present, forensic scientists carried out many studies on the genetic polymorphisms of the Mongolian group. And these studies were based on different molecular genetic markers, such as autosomal STRs (Fang et al, 2019;Gao et al, 2014;Wen et al, 2019;Zha et al, 2014), X-Chromosome STR (Chen et al, 2018), Y-STR (Fu et al, 2016;Gao et al, 2016;Wang et al, 2019), human leukocyte antigen (Gu, Chen, Yao, Sun, & Shi, 2019;Hong et al, 2007), SNPs (Brissenden et al, 2015). These studies were of great reference values for the Mongolian group genetic research, while research on the Y-STR haplotype data in XJM group is still insufficient.…”

Insights From Y-STRs: Forensic Characteristics, Genetic Affinities, and Linguistic Classifications of Guangdong Hakka and She Groups

“…From the dimensionality reduction analyses discussed above, the genetic relationships between Guangdong She and other Chinese populations were not clarified. In addition, we employed the Y-STR haplotype profiles of 42 Chinese minorities and Guangdong She to assess the population pairwise genetic distances by AMOVA ( Zhu et al, 2005 , 2006a , b , 2008 ; He and Guo, 2013 ; Shan et al, 2014 ; Luo et al, 2015 , 2019 ; Ou et al, 2015 ; Shu et al, 2015 ; Bai et al, 2016a ; Bian et al, 2016 ; Fu et al, 2016 ; Gao et al, 2016 ; Yao et al, 2016 ; Guo et al, 2017 ; Ji et al, 2017 ; Zhao et al, 2017 ; Cao et al, 2018 ; Chen et al, 2018 ; Liu Y. J. et al, 2018 ; Atif et al, 2019 ; Liu et al, 2019 ; Xie et al, 2019 ; Dezhi et al, 2020 ; Feng et al, 2020 ; Song et al, 2020 ; Tang et al, 2020 ). The pairwise R ST and the corresponding p values between Guangdong She and Chinese minority populations from five linguistically different families are displayed in Supplementary Table 7 .…”