Genetic polymorphisms in cyclin D1 are associated with risk of renal cell cancer in the Chinese population

Xue, Jianxin; Qin, Zhiqiang; Li, Xiao; Zhang, Jianzhong; Zheng, Yuxiao; Xu, Weizhang; Cao, Qiang; Wang, Zengjun

doi:10.18632/oncotarget.20720

Cited by 9 publications

(7 citation statements)

References 35 publications

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“…Previous research has demonstrated that ccnd1 serves an important role in promoting the development and progress of multiple types of human cancer, such as lung adenocarcinoma, glioma and renal cell cancer (46)(47)(48)(49). a previous study indicated that mir-374a inactivated the phosphoinositide 3-kinase (Pi3K)/aKT signaling axis through inhibiting ccnd1 and suppressing the progression of colon cancer (49).…”

Section: Ccnd1 Is Required For the Proliferation And Apoptosis Of Ht-mentioning

confidence: 99%

MicroRNA‑628‑5p inhibits cell proliferation and induces apoptosis in colorectal cancer through downregulating CCND1 expression levels

Guo

Xue

2020

Mol Med Report

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Microrna (mir)-628-5p serves as an antitumor gene in a variety of cancers; however, the role of mir-628-5p in colorectal cancer remains largely unclear. The purpose of this study was to investigate the role and mechanism of mir-628-5p in colorectal cancer. reverse transcription-quantitative Pcr (RT-qPCR), colony formation assays and flow cytometric analysis were used to determine the expression levels of mir-628-5p in colorectal cancer tissues and cell lines, and the proliferative ability of colorectal cancer cells. TargetScan version 7.2 and dual-luciferase reporter assay were performed to predict and confirm mir-628-5p target genes. The expression levels of cyclin d1 (ccnd1) and related genes were determined using rT-qPcr or/and western blotting analysis. mir-628-5p mimics and ccnd1 plasmids were used to overexpress mir-628-5p and ccnd1; it was demonstrated that the expression levels of miR-628-5p were significantly downregulated in colorectal cancer tissues and cell lines. mir-628-5p mimic-transfected cells inhibited the proliferation and induced apoptosis of HT-29 cells. ccnd1, a downstream effector of mir-628-5p, promoted the proliferation and suppressed apoptosis of HT-29 cells, and the effects were reversed by mir-628-5p mimics. in conclusion, the present study suggested that colorectal cancer progression may be regulated through the mir-628-5p/ccnd1 axis, and mir-628-5p could be used as a potential diagnostic and prognostic biomarker for colorectal cancer.

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Section: Ccnd1 Is Required For the Proliferation And Apoptosis Of Ht-mentioning

confidence: 99%

MicroRNA‑628‑5p inhibits cell proliferation and induces apoptosis in colorectal cancer through downregulating CCND1 expression levels

Guo

Xue

2020

Mol Med Report

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“…cyclin d1 (ccnd1), a member of the highly conserved cyclin family, can function as a modulator of cyclin-dependent kinase (cdK)4 or cdK6, and has an important role in adjusting the progression of the cell cycle (32,33). Previous studies revealed that ccnd1 has a crucial role in promoting proliferation, migration and tumor metastasis (34,35) in cancers such as lung cancer (33), gastric cancer (36) and renal cell cancer (37). ccnd1 has been reported to exhibit a high expression in Tc tissues and to be associated with invasive behavior (38).…”

Section: Introductionmentioning

confidence: 99%

Downregulation of miR‑193a‑3p via targeting cyclin D1 in thyroid cancer

Wen

et al. 2020

Mol Med Rep

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Thyroid cancer (Tc) is a frequently occurring malignant tumor with a rising steadily incidence. microrna (mirna/mir)-193a-3p is an mirna that is associated with tumors, playing a crucial role in the genesis and progression of various cancers. However, the expression levels of mir-193a-3p and its molecular mechanisms in Tc remain to be elucidated. The present study aimed to probe the expression of miR-193a-3p and its clinical significance in TC, including its underlying molecular mechanisms. Microarray and rna sequencing data gathered from three major databases, specifically Gene expression omnibus (Geo), arrayexpress and The cancer Genome atlas (TcGa) databases, and the relevant data from the literature were used to examine mir-193a-3p expression. Meta-analysis was also conducted to evaluate the association between clinicopathological parameters and mir-193a-3p in 510 Tc and 59 normal samples from the TcGa database. mirWalk 3.0, and the TcGa and Geo databases were used to predict the candidate target genes of mir-193a-3p. Gene ontology, Kyoto encyclopedia of Genes and Genomes and protein-protein interaction network enrichment analyses were conducted by using the predicted candidate target genes to investigate the underlying carcinogenic mechanisms. a dual luciferase assay was performed to validate the targeting regulatory association between the most important hub gene cyclin d1 (ccnd1) and mir-193a-3p. mir-193a-3p expression was considerably downregulated in Tc compared with in the non-cancer controls (P<0.001). The area under the curve of the summary receiver operating characteristic was 0.80. Downregulation of miR-193a-3p was also significantly associated with age, sex and metastasis (P=0.020, 0.044 and 0.048, respectively). Bioinformatics analysis indicated that a low mir-193a-3p expression may augment ccnd1 expression to affect the biological processes of Tc. in addition, ccnd1, as a straightforward target, was validated through a dual luciferase assay. mir-193a-3p and ccnd1 may serve as prognostic biomarkers of Tc. Finally, mir-193a-3p may possess a crucial role in the genesis and progression of Tc by altering the ccnd1 expression.

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“…Cyclin D1 gene, second important molecule of cell cycle pathway has also been found mutated in almost every human cancer by altering the normal cell cycle pathway. CCND1 gene polymorphisms have been found in several cancers [38][39][40][41][42][43][44][45][46][47] including gliomas also [48]. However, till now no study has been published with respect to brain tumor and different subtypes of brain tumor such as glioma, meningioma and pituitary adenoma.…”

Section: Discussionmentioning

confidence: 99%

Genetic and expression variations of cell cycle pathway genes in brain tumor patients

et al. 2020

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The present study was designed to determine the association between the genetic polymorphisms/expression variations of RB1 and CCND1 genes and brain tumor risk. For this purpose, 250 blood samples of brain tumor patients along with 250 controls (cohort I) and 96 brain tumor tissues (cohort II) with adjacent control section were collected. Mutation analysis of RB1 (rs137853294, rs121913300) and CCND1 (rs614367, rs498136) genes was performed using ARMS-PCR followed by sequencing, and expression analysis was performed using real-time PCR and immunohistochemistry. The results showed homozygous mutant genotype of RB1 gene polymorphism, rs121913300 (P=0.003) and CCND1 gene polymorphism rs614367 (P=0.01) were associated significantly with brain tumor risk. Moreover, significant down-regulation of RB1 (P=0.005) and up-regulation of CCND1 (P=0.0001) gene was observed in brain tumor sections vs controls. Spearman correlation showed significant negative correlation between RB1 vs proliferation marker, Ki-67 (r = −0.291*, P<0.05) in brain tumors. Expression levels of selected genes were also assessed at protein level using immunohistochemical analysis (IHC) and signification down-regulation of RB1 (P=0.0001) and up-regulation of CCND1 (P=0.0001) was observed in brain tumor compared with control sections. In conclusion, it is suggested that polymorphisms/expression variations of RB1 and CCND1 genes may be associated with increased risk of brain tumor.

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Genetic polymorphisms in cyclin D1 are associated with risk of renal cell cancer in the Chinese population

Cited by 9 publications

References 35 publications

MicroRNA‑628‑5p inhibits cell proliferation and induces apoptosis in colorectal cancer through downregulating CCND1 expression levels

MicroRNA‑628‑5p inhibits cell proliferation and induces apoptosis in colorectal cancer through downregulating CCND1 expression levels

Downregulation of miR‑193a‑3p via targeting cyclin D1 in thyroid cancer

Genetic and expression variations of cell cycle pathway genes in brain tumor patients

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