Genetic Polymorphisms in ADORA2A and CYP1A2 Influence Caffeine’s Effect on Postprandial Glycaemia

Abstract

The liver enzyme cytochrome P450 1A2 (CYP1A2) is responsible for 90% of caffeine metabolism, while caffeine exerts many of its effects via antagonist binding to adenosine A2a receptors (ADORA2A). This study aimed to examine whether functional single nucleotide polymorphisms (SNPs) in 1976T > C (ADORA2A; rs5751876) and −163C > A (CYP1A2; rs762551) influence the effect of caffeine on the postprandial glucose (GLU) response to a carbohydrate meal. We report that individuals with the 1976T > C CC, but not CT/TT genotypes display elevated GLU levels after consuming caffeine and carbohydrate (CHO + CAFF) versus carbohydrate only (CHO). The GLU area under the curve (AUC) was also greater during the CHO + CAFF condition compared to the CHO condition in CC, but not the CT/TT genotypes. The −163C > A AC/CC, but not AA, genotypes displayed greater GLU concentrations 60-min post meal during CHO + CAFF versus CHO. Our data suggest that caffeine-induced impairments in postprandial glycaemia are related to 1976T > C and −163C > A SNPs.

Enhanced long-term dietary change and adherence in a nutrigenomics-guided lifestyle intervention compared to a population-based (GLB/DPP) lifestyle intervention for weight management: results from the NOW randomised controlled trial

“…This genotyping method has been used in previous research. [21][22][23] The following single nucleotide polymorphisms of interest to the current dietary change and adherence study were analysed: UCP1 (rs1800592), FTO (rs9939609), TCF7L2 (rs7903146), APOA2 (rs5082), PPARγ2 (rs1801282), and MC4R (rs17782313).…”

Glucose control upon waking is unaffected by hourly sleep fragmentation during the night, but is impaired by morning caffeinated coffee

“…Caffeine is primarily metabolised (>95 %) through CYP1A2 activity (13) . However, this modulating role has yet to be investigated under a variety of scenarios (14,15) .…”

Caffeine and Exercise Performance: Possible Directions for Definitive Findings

“…A single recent study (Banks et al, 2019 ) correlated caffeine-induced metabolic change to different ADORA2A genotypes. The study showed that healthy men carriers of the C/C genotype have less postprandial glucose uptake after consuming caffeine (4 mg.kg −1 ) and carbohydrate (0.75 g CHO.Kg −1 ) when compared to only carbohydrate intake (0.75 g CHO.Kg −1 ).…”

“…The study showed that healthy men carriers of the C/C genotype have less postprandial glucose uptake after consuming caffeine (4 mg.kg −1 ) and carbohydrate (0.75 g CHO.Kg −1 ) when compared to only carbohydrate intake (0.75 g CHO.Kg −1 ). In opposition to the C/C genotype, T-carriers have the same response to glucose uptake with or without caffeine administration (Banks et al, 2019 ). Even not using performance tests, Banks et al ( 2019 ) indicate that genetic factors related to ADORA2A interfere with glucose mobilization after caffeine consumption.…”

“…In opposition to the C/C genotype, T-carriers have the same response to glucose uptake with or without caffeine administration (Banks et al, 2019 ). Even not using performance tests, Banks et al ( 2019 ) indicate that genetic factors related to ADORA2A interfere with glucose mobilization after caffeine consumption. This could indicate that genetic backgrounds may influence the contradictory effects of caffeine on muscle glucose metabolism (glucose uptake and increase muscle glycogen stocks) (Graham and Spriet, 1991 ; Spriet et al, 1992 ; Gonzalez and Stevenson, 2012 ).…”