Genetic polymorphism of plasma α₁B‐glycoprotein and transferrin in arctic and silver foxes

Abstract

Plasma samples of 235 foxes from 38 complete families (14 of arctic foxes, 21 of silver foxes and 3 with arctic X silver fox hybrid offspring) were analysed by one-dimensional horizontal polyacrylamide gel electrophoresis (PAGE) pH 9.0 followed by general-protein staining of gels. A major postalbumin of fox plasma was identified as alB-glycoprotein (alB) by using immunoblotting with antiser m specific to human or pig plasma a l B . Four codominant, autosomal alleles of a , B were found in arctic foxes. Two transferrin (TF) alleles (Tf ", Tf") were observed in arctic foxes and two ( T f D , T f f ) in silver foxes; the TF F type of both of the fox species showed identical electrophoretic mobilities. The arctic foxes showed a high degree of polymorphism for both TF and alB. The silver foxes showed a scarce polymorphism of TF and were monomorphic for alB. The arctic fox, silver fox and their hybrids could be clearly differentiated from one another by their plasma protein patterns obtained by the PAGE method.

Two‐dimensional electrophoresis of the plasma proteins of alpacas and llamas: genetic polymorphism of α1B‐glycoprotein and three other proteins

“…Inheritance data (Table 3) supported the hypothesis that the observed phenotypes were controlled by five codominant alleles, A , C , D , Fand S , at an autosomal locus. As in humans and other animal species Juneja et al 1987Juneja et al , 1988, we propose the locus symbol A I B for alB-glycoprotein in alpacas and llamas. By protein staining, A1B A, D and S variants each consisted of one major and one minor fraction, while A1B C and F variants consisted only of a single major fraction.…”

Three new plasma protein polymorphisms in domestic foxes, detected by a simple method of 2D horizontal electrophoresis

“…The details of the foxes investigated and of blood sampling were given previously (JUNEJA et al 1988). Seven additional families of silver foxes were included in this study.…”

“…The relative mobilities of ten different plasma proteins are shown. The identification of prealbumin (Pr), albumin (Alb), Gc protein and a,B-glycoprotein (a,B) was according to JUNEJA et al (1988). The postalbumin, Po, described by JUNEJA et al (1988) is redesignated as postalbumin 2 (Pa2).…”

“…The identification of prealbumin (Pr), albumin (Alb), Gc protein and a,B-glycoprotein (a,B) was according to JUNEJA et al (1988). The postalbumin, Po, described by JUNEJA et al (1988) is redesignated as postalbumin 2 (Pa2). Two other proteins were observed to be serine protease inhibitors in this study (data not given here); these were designated protease inhibitor 1 (Pil) and protease inhibitor 2 (Pi2) ( Fig.…”

“…As compared to other farm animals, only a few blood protein polymorphisms have previously been reported in these species. These are of plasma a,B-glycoprotein (a,B), transferrin (Tf), and prealbumin (Pr) in arctic foxes, and of Tf, haptoglobin (Hp), and red cell arylesterase (Es-1) in silver foxes (SEROV et al 1973(SEROV et al , 1974BALBIERZ et al 1977;KASTANOV and MASHUROV1983;JUNEJA et al 1988). Previously, by using a simple method of twodimensional (2D) horizontal electrophoresis, we reported several new plasma protein polymorphisms in different animal species (e.g., JUNEJA et al 1983 and.…”