Genetic polymorphism of miRNA-196a and its target gene annexin-A1 expression based on ethnicity in Pakistani female breast cancer patients

Rahim, Amena; Afzal, Muhammad; Naveed, Abdul Khaliq

doi:10.12669/pjms.35.6.1322

Cited by 8 publications

(7 citation statements)

References 24 publications

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“…14,15 It can reach tumor cells in the vascular endothelial space and undergo a liquid-to-gas phase transition to enhance the ultrasound imaging, so it has been widely used in ultrasound molecular imaging. [16][17][18][19] You et al 20 Prepared a multifunctional Fe3O4-PFH/PLGA nanoparticle, which undergoes a phase change in the PFH core of highly focused ultrasound and can work in synergy with highintensity focused ultrasound to effectively improve the therapeutic effect of tumors. In this study, in vitro targeting experiments showed that many nanoparticles labeled with red fluorescence in the AP-GNP group were tightly attached to the cell membrane surface of MCF-7 labeled with green fluorescence, and some nanoparticles were swallowed.…”

Section: Discussionmentioning

confidence: 99%

Application of ultrasound molecular imaging based on compressed sensing reconstruction algorithm to phase change drug-loaded PLGA nanoparticles targeting breast cancer MCF-7 Cells

2021

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Objectives: To study the ability of aptamer-modified nano-gold rods and liquid carbon-targeted PLGA nanoparticles to target in vitro using compressed sensing reconstruction algorithm, and observe the phenomenon of mediating ultrasound / photoacoustic imaging. Methods: PLGA nanoparticles were prepared by a double emulsification method, and the MUC1 aptamer was connected to the PLGA nanoparticles by the carbodiimide method to obtain an “aptamer-PLGA nanoparticle” targeted phase change contrast agent. Fluorescence microscopy was used to detect the in vitro targeting of breast cancer MCF-7 cells specifically identified by it, and three control groups were set up: the ordinary nanoparticle group, the aptamer interference group, and the HELA cell group. A photoacoustic instrument was used to observe the phenomenon of enhanced ultrasound / photoacoustic signal mediated in vitro. Results: Many targeted nanoparticles were clustered around MCF-7 cells and bound firmly, but no specific binding was observed in the non-targeted nanoparticles group, the aptamer interference group and the HELA cell group. After the targeted nanoparticle was excited by the photoacoustic instrument, the ultrasonic signal and the photoacoustic signal were significantly enhanced compared with before the excitation. Conclusion: The successfully prepared targeting nanoparticles have good targeting and specificity for breast cancer MCF-7 cells, and it has obvious effects on ultrasound / photoacoustic imaging, and has the potential to become a dual-mode ultrasound / photoacoustic targeted contrast agent. The various characteristics provide experimental basis for subsequent in vivo targeting experiments and are expected to become good target diagnostic molecular probes. doi: https://doi.org/10.12669/pjms.37.6-WIT.4852 How to cite this:You Y, Cheng W, Chen H. Application of ultrasound molecular imaging based on compressed sensing reconstruction algorithm to phase change drug-loaded PLGA nanoparticles targeting breast cancer MCF-7 Cells. Pak J Med Sci. 2021;37(6):1610-1614. doi: https://doi.org/10.12669/pjms.37.6-WIT.4852 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Section: Discussionmentioning

confidence: 99%

Application of ultrasound molecular imaging based on compressed sensing reconstruction algorithm to phase change drug-loaded PLGA nanoparticles targeting breast cancer MCF-7 Cells

2021

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“…Furthermore, one of the first discovered miRNA, miRNA-196a, which is upregulated in the vast majority of cancer types including NSCLC, was shown to be involved in the mediation of cisplatin resistance; however, its mechanism is not clear [75]. MiRNA-196a targets the Annexin-A1 (ANXA1) gene that regulates physiological mechanisms such as hormone secretion, apoptosis, exocytosis, and signal transduction [76]. ANXA1 is also involved in the acquisition and maintenance of a cancer stem cell-like phenotype that is characterised by upregulation of several chemoresistant mechanisms, including the activity of ABC proteins [77,78].…”

Section: Epigenetic Regulation By Mirnasmentioning

confidence: 99%

Molecular Mechanisms of Chemoresistance Induced by Cisplatin in NSCLC Cancer Therapy

Kryczka

Czarnecka-Chrebelska

et al. 2021

IJMS

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Cancer cells utilise several mechanisms to increase their survival and progression as well as their resistance to anticancer therapy: deregulation of growth regulatory pathways by acquiring grow factor independence, immune system suppression, reducing the expression of antigens activating T lymphocyte cells (mimicry), induction of anti-apoptotic signals to counter the action of drugs, activation of several DNA repair mechanisms and driving the active efflux of drugs from the cell cytoplasm, and epigenetic regulation by microRNAs (miRNAs). Because it is commonly diagnosed late, lung cancer remains a major malignancy with a low five-year survival rate; when diagnosed, the cancer is often highly advanced, and the cancer cells may have acquired drug resistance. This review summarises the main mechanisms involved in cisplatin resistance and interactions between cisplatin-resistant cancer cells and the tumour microenvironment. It also analyses changes in the gene expression profile of cisplatin sensitive vs. cisplatin-resistant non-small cell lung cancer (NSCLC) cellular model using the GSE108214 Gene Expression Omnibus database. It describes a protein-protein interaction network that indicates highly dysregulated TP53, MDM2, and CDKN1A genes as they encode the top networking proteins that may be involved in cisplatin tolerance, these all being upregulated in cisplatin-resistant cells. Furthermore, it illustrates the multifactorial nature of cisplatin resistance by examining the diversity of dysregulated pathways present in cisplatin-resistant NSCLC cells based on KEGG pathway analysis.

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“…ANXA1 plays a significant role in membrane trafficking, exocytosis, signal transduction, cell differentiation, and apoptosis, among other biological roles ( Luthra et al, 2008 ). Overexpression of miR-196a2 due to variant rs11614913 leads to the suppression of ANXA1 thereby promoting cell proliferation and suppressing apoptosis ( Solito et al, 2001 ; Luthra et al, 2008 ; Rahim et al, 2019 ). ANXA1 exhibits varied expression in different cancers.…”

Section: Snps In the Seed Sequence Of Mirna And The 3′utr Of Specific Target Gene In Cancermentioning

confidence: 99%

“…ANXA1 exhibits varied expression in different cancers. It is upregulated in glioma and oropharyngeal cancer and downregulated in prostate cancer, esophageal squamous cell, and head and neck squamous carcinoma ( Rahim et al, 2019 ). As an upstream regulator, miR-196a has been found to partially direct the cleavage of the mRNA of the HOX gene clusters.…”

Section: Snps In the Seed Sequence Of Mirna And The 3′utr Of Specific Target Gene In Cancermentioning

confidence: 99%

SNPs in miRNAs and Target Sequences: Role in Cancer and Diabetes

et al. 2021

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miRNAs are fascinating molecular players for gene regulation as individual miRNA can control multiple targets and a single target can be regulated by multiple miRNAs. Loss of miRNA regulated gene expression is often reported to be implicated in various human diseases like diabetes and cancer. Recently, geneticists across the world started reporting single nucleotide polymorphism (SNPs) in seed sequences of miRNAs. Similarly, SNPs are also reported in various target sequences of these miRNAs. Both the scenarios lead to dysregulated gene expression which may result in the progression of diseases. In the present paper, we explore SNPs in various miRNAs and their target sequences reported in various human cancers as well as diabetes. Similarly, we also present evidence of these mutations in various other human diseases.

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Genetic polymorphism of miRNA-196a and its target gene annexin-A1 expression based on ethnicity in Pakistani female breast cancer patients

Cited by 8 publications

References 24 publications

Application of ultrasound molecular imaging based on compressed sensing reconstruction algorithm to phase change drug-loaded PLGA nanoparticles targeting breast cancer MCF-7 Cells

Application of ultrasound molecular imaging based on compressed sensing reconstruction algorithm to phase change drug-loaded PLGA nanoparticles targeting breast cancer MCF-7 Cells

Molecular Mechanisms of Chemoresistance Induced by Cisplatin in NSCLC Cancer Therapy

SNPs in miRNAs and Target Sequences: Role in Cancer and Diabetes

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