Genetic polymorphism of growth hormone releasing hormonegene in exotic and crossbred pigs

Abstract

Growth hormone releasing hormone (GHRH) plays a central role in growth and production through its influence on important metabolic activities in mammals which prompted its wide commercial use including use as a candidate marker. In present experiment, polymorphism at exon 2 and 3 of Growth Hormone Releasing Hormone gene (GHRH) was studied using PCR-RFLP in crossbred and exotic pigs, commonly found in north east India. Restriction enzyme digestion of 467 bp PCR amplicon with AluI revealed 1 genotype. Comparison of obtained sequences revealed large scale nucleotide and residue substitutions. Observed polymorphism may be associated with wide variability in growth (rate) of different breeds of pig.

Three SINE Insertion Polymorphic Sites were Identified in Insulin-like Growth Factor 2 mRNA-binding Proteins

“…Primers were synthesized by Nanjing Kinco Biotech Co., Ltd (Nanjing, China) and their coordinates in the reference genome, annealing temperature and sequences were supplied in Table S2. The PCR (Kaushik et al 2017) amplification time was determined according to the target fragment size.…”

Performance of Ghungroo and Ghungroo × Hampshire pigs under low input production system in climatic condition of Garo hills of Meghalaya

“…Pourouchottamane et al (2013) reported similar observations with the present findings in case of Ghungroo (8.758±0.542 kg and 46.83±3.597kg) pigs. Kaushik et al (2013) reported higher birth weight of GH × HS pigs in organised farm. There was significant (P<0.05) difference in body weight at 6 months and 9 months of age in Ghungroo (34.28±0.59 kg and 44.21±0.97 kg), Ghungroo × Hampshire (48.91±0.55 kg and 65.30±1.37 kg) and nondescript local (21.52±0.36 kg and 36.67±0.88 kg).…”