Genetic polymorphism of Algerian Leishmania infantum strains revealed by multilocus microsatellite analysis

Seridi, Nabila; Amro, Ahmad; Kuhls, Katrin; Belkaid, M; Zidane, Charef; Al-Jawabreh, Amer; Schönian, Gabriele

doi:10.1016/j.micinf.2008.07.031

Cited by 47 publications

(48 citation statements)

References 26 publications

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“…Moreover, a strong population structure was found at a micro-geographical scale as the populations within the different countries were genetically heterogenous. This is in accord with the results of earlier population studies of L. infantum Seridi et al 2008;Amro et al 2009;Chargui et al 2009), L. tropica (Schwenkenbecher et al 2006), and L. major . Recent research by MLMT on Sudanese L. donovani showed populations consistent with inbreeding, although it also detected the first population without departure from Hardy-Weinberg equilibrium (Baleela, personal communication).…”

supporting

confidence: 92%

Molecular approaches for a better understanding of the epidemiology and population genetics ofLeishmania

2010

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S U M M A R YMolecular approaches are being used increasingly for epidemiological studies of visceral and cutaneous leishmaniases. Several molecular markers resolving genetic differences between Leishmania parasites at species and strain levels have been developed to address key epidemiological and population genetic questions. The current gold standard, multilocus enzyme typing (MLEE), needs cultured parasites and lacks discriminatory power. PCR assays identifying species directly with clinical samples have proven useful in numerous field studies. Multilocus sequence typing (MLST) is potentially the most powerful phylogenetic approach and will, most probably, replace MLEE in the future. Multilocus microsatellite typing (MLMT) is able to discriminate below the zymodeme level and seems to be the best candidate for becoming the gold standard for distinction of strains. Population genetic studies by MLMT revealed geographical and hierarchic population structure in L. tropica, L. major and the L. donovani complex. The existence of hybrids and gene flow between Leishmania populations suggests that sexual recombination is more frequent than previously thought. However, typing and analytical tools need to be further improved. Accessible databases should be created and sustained for integrating data obtained by different researchers. This would allow for global analyses and help to avoid biases in analyses due to small sample sizes.

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confidence: 92%

Molecular approaches for a better understanding of the epidemiology and population genetics ofLeishmania

2010

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“…However, another study conducted using Algerian strains of L . infantum reported no association between MON profiles and MLMT profiles [30]. Our results also support the idea that MLMT is the best candidate to be accepted as gold standard by having further discriminatory power [31].…”

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confidence: 85%

Epidemiological analysis of Leishmania tropica strains and giemsa-stained smears from Syrian and Turkish leishmaniasis patients using multilocus microsatellite typing (MLMT)

et al. 2017

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Turkey is located in an important geographical location, in terms of the epidemiology of vector-borne diseases, linking Asia and Europe. Cutaneous leishmaniasis (CL) is one of the endemic diseases in a Turkey and according to the Ministry Health of Turkey, 45% of CL patients originate from Şanlıurfa province located in southeastern Turkey. Herein, the epidemiological status of CL, caused by L. tropica, in Turkey was examined using multilocus microsatellite typing (MLMT) of strains obtained from Turkish and Syrian patients. A total of 38 cryopreserved strains and 20 Giemsa-stained smears were included in the present study. MLMT was performed using 12 highly specific microsatellite markers. Delta K (ΔK) calculation and Bayesian statistics were used to determine the population structure. Three main populations (POP A, B and C) were identified and further examination revealed the presence of three subpopulations for POP B and C. Combined analysis was performed using the data of previously typed L. tropica strains and Mediterranean and Şanlıurfa populations were identified. This finding suggests that the epidemiological status of L. tropica is more complicated than expected when compared to previous studies. A new population, comprised of Syrian L. tropica samples, was reported for the first time in Turkey, and the data presented here will provide new epidemiological information for further studies.

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“…However, if the marker considered has a fast molecular clock, and generates much diversity in a short time, this feature may not be observed e all individuals would have a different MLG even in the case of PCE (ArnaudHaond et al, 2005;Seridi et al, 2008). As we will see further ('Identical Multilocus Genotypes is a Relative Notion'), this notion of repeated genotypes strongly depends on the mutation rate of the marker considered.…”

Section: Repeated Multilocus Genotypes That Are Overrepresented Undermentioning

confidence: 90%

Is Predominant Clonal Evolution a Common Evolutionary Adaptation to Parasitism in Pathogenic Parasitic Protozoa, Fungi, Bacteria, and Viruses?

Tibayrenc

Ayala

2017

Advances in Parasitology

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Genetic polymorphism of Algerian Leishmania infantum strains revealed by multilocus microsatellite analysis

Cited by 47 publications

References 26 publications

Molecular approaches for a better understanding of the epidemiology and population genetics ofLeishmania

Molecular approaches for a better understanding of the epidemiology and population genetics ofLeishmania

Epidemiological analysis of Leishmania tropica strains and giemsa-stained smears from Syrian and Turkish leishmaniasis patients using multilocus microsatellite typing (MLMT)

Is Predominant Clonal Evolution a Common Evolutionary Adaptation to Parasitism in Pathogenic Parasitic Protozoa, Fungi, Bacteria, and Viruses?

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