Genetic point‐of‐care diagnosis of <i><scp>M</scp></i><i>ycoplasma pneumoniae</i> infection using <scp>LAMP</scp> assay

Kakuya, Fujio; Kinebuchi, Takahiro; Fujiyasu, Hiroaki; Tanaka, Ryosuke; Kano, Hiroki

doi:10.1111/ped.12327

Cited by 19 publications

(18 citation statements)

References 20 publications

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“…LAMP has been evaluated in several studies in various formats for detection of M. pneumoniae in OP swabs, including comparisons with serology, conventional, and real-time PCR, in Japan, China, and the United States (307-314), with generally favorable results in terms of diagnostic sensitivity. LAMP technology is now recommended as a first-line diagnostic method for detecting acute M. pneumoniae infections in Japan (310).…”

Section: Nucleic Acid Amplificationmentioning

confidence: 99%

Mycoplasma pneumoniae from the Respiratory Tract and Beyond

et al. 2017

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is an important cause of respiratory tract infections in children as well as adults that can range in severity from mild to life-threatening. Over the past several years there has been much new information published concerning infections caused by this organism. New molecular-based tests for detection are now commercially available in the United States, and advances in molecular typing systems have enhanced understanding of the epidemiology of infections. More strains have had their entire genome sequences published, providing additional insights into pathogenic mechanisms. Clinically significant acquired macrolide resistance has emerged worldwide and is now complicating treatment. susceptibility testing methods have been standardized, and several new drugs that may be effective against this organism are undergoing development. This review focuses on the many new developments that have occurred over the past several years that enhance our understanding of this microbe, which is among the smallest bacterial pathogens but one of great clinical importance.

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Section: Nucleic Acid Amplificationmentioning

confidence: 99%

Mycoplasma pneumoniae from the Respiratory Tract and Beyond

et al. 2017

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“…Loop‐mediated isothermal amplification detection of various pathogens has been reported for many infections, including Mycobacterium tuberculosis , Mycoplasma pneumoniae , and Streptococcus pneumoniae . We recently reported almost identical specificity and superior sensitivity of LAMP over PCR for the detection of Campylobacter spp.…”

Section: Discussionmentioning

confidence: 93%

Rapid and simple detection of Ureaplasma species from vaginal swab samples using a loop‐mediated isothermal amplification method

Fuwa

Seki

Hirata

et al. 2017

American J Rep Immunol

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“…A similarly rapid detection method for M. pneumoniae known as loop-mediated isothermal amplification (LAMP) has been shown to have comparable sensitivity to RAA [25][26][27]. However, the current LAMP methods, especially LAMP with two loop primers, suffer from undesired nonspecific amplification with strong background signals due to the increasing target sites.…”

Section: Discussionmentioning

confidence: 99%

Use of a rapid recombinase-aided amplification assay for Mycoplasma pneumoniae detection

Xue

Zhao

et al. 2020

BMC Infect Dis

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Background: Mycoplasma pneumoniae is one of the most common causative pathogens of community-acquired pneumonia (CAP), accounting for as many as 30-50% of CAP during peak years. An early and rapid diagnostic method is key for guiding clinicians in their choice of antibiotics. Methods: The recombinase-aided amplification (RAA) assay is a recently developed, rapid detection method that has been used for the detection of several pathogens. The assays were performed in a one-step single tube reaction at 39°Celsius within 15-30 min. In this study, we established an RAA assay for M. pneumoniae using clinical specimens for validation and commercial real-time PCR as the reference method. Results: The analytical sensitivity of the RAA assay was 2.23 copies per reaction, and no cross-reactions with any of the other 15 related respiratory bacterial pathogens were observed. Compared with the commercial real-time PCR assay used when testing 311 respiratory specimens, the RAA assay obtained 100% sensitivity and 100% specificity with a kappa value of 1. Conclusions: These results demonstrate that the proposed RAA assay will be of benefit as a faster, sensitive, and specific alternative tool for the detection of M. pneumoniae.

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Genetic point‐of‐care diagnosis of Mycoplasma pneumoniae infection using LAMP assay

Abstract: LAMP assay had excellent sensitivity and specificity for the detection of acute MP infection at the first visit. This assay can diagnose MP infection during the very acute phase. LAMP assay is appropriate for genetic point-of-care diagnosis of MP infection in hospital laboratories.

Cited by 19 publications

References 20 publications

Mycoplasma pneumoniae from the Respiratory Tract and Beyond

Mycoplasma pneumoniae from the Respiratory Tract and Beyond

Rapid and simple detection of Ureaplasma species from vaginal swab samples using a loop‐mediated isothermal amplification method

Use of a rapid recombinase-aided amplification assay for Mycoplasma pneumoniae detection

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