Genetic parameter estimates and identification of SNPs associated with growth traits in Senegalese sole

Guerrero-Cózar, Israel; Jiménez-Fernández, Eduardo; Berbel, Concha; Córdoba-Caballero, José; Claros, M. Gonzalo; Zerolo, Ricardo; Manchado, Manuel

doi:10.1016/j.aquaculture.2021.736665

Cited by 9 publications

(8 citation statements)

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“…The temperature seems to be a major factor that modifies sex ratios during larval development generating skewed populations of neomales and neofemales 62 , 63 . Familial sex ratios in sole were reported to oscillate from 16 up to 90% males supporting a high impact of environmental factors to modulate sex differentiation and sex population ratios 19 .…”

Section: Discussionmentioning

confidence: 88%

“…Soles used for the preparation of ddRAD libraries and sequencing were selected from the genetic breeding program carried out by the IFAPA in collaboration with a commercial aquaculture company (CUPIMAR S.A.). Production of families used in this study, genotyping and parentage assignment were previously published 19 , 20 . Five families (three full-sib and two maternal half-sib families) containing between 48 and 96 individuals per family (total n = 356) were selected to construct the genetic linkage map (Table 1 ).…”

Section: Methodsmentioning

confidence: 99%

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Chromosome anchoring in Senegalese sole (Solea senegalensis) reveals sex-associated markers and genome rearrangements in flatfish

Guerrero-Cózar

Gómez‐Garrido

Berbel

et al. 2021

Sci Rep

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The integration of physical and high-density genetic maps is a very useful approach to achieve chromosome-level genome assemblies. Here, the genome of a male Senegalese sole (Solea senegalensis) was de novo assembled and the contigs were anchored to a high-quality genetic map for chromosome-level scaffolding. Hybrid assembled genome was 609.3 Mb long and contained 3403 contigs with a N50 of 513 kb. The linkage map was constructed using 16,287 informative SNPs derived from ddRAD sequencing in 327 sole individuals from five families. Markers were assigned to 21 linkage groups with an average number of 21.9 markers per megabase. The anchoring of the physical to the genetic map positioned 1563 contigs into 21 pseudo-chromosomes covering 548.6 Mb. Comparison of genetic and physical distances indicated that the average genome-wide recombination rate was 0.23 cM/Mb and the female-to-male ratio 1.49 (female map length: 2,698.4 cM, male: 2,036.6 cM). Genomic recombination landscapes were different between sexes with crossovers mainly concentrated toward the telomeres in males while they were more uniformly distributed in females. A GWAS analysis using seven families identified 30 significant sex-associated SNP markers located in linkage group 18. The follicle-stimulating hormone receptor appeared as the most promising locus associated with sex within a region with very low recombination rates. An incomplete penetrance of sex markers with males as the heterogametic sex was determined. An interspecific comparison with other Pleuronectiformes genomes identified a high sequence similarity between homologous chromosomes, and several chromosomal rearrangements including a lineage-specific Robertsonian fusion in S. senegalensis.

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Section: Discussionmentioning

confidence: 88%

Section: Methodsmentioning

confidence: 99%

Chromosome anchoring in Senegalese sole (Solea senegalensis) reveals sex-associated markers and genome rearrangements in flatfish

Guerrero-Cózar

Gómez‐Garrido

Berbel

et al. 2021

Sci Rep

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“…They were fed with frozen feed including mussels, small squids and polychaeta worms (Seabait Ltd., Ashington, UK) on alternative days. Mass spawning strategy to create the families was previously described [13]. Briefly, spawning was synchronized by thermoperiod control [14].…”

Section: Animalsmentioning

confidence: 99%

“…For genetic evaluation, fish (ranging from 200 to 550 specimens per EB) were intraperitoneally tagged, with ages ranging between 150 and 278 days post-hatch (dph) as previously reported [13,18]. Later, fish were phenotypically evaluated in vivo at~400 d (ranging from 395 to 446 dph) before entering the growth-out period in RAS and at harvest age~800 d (ranging between 733 and 861 dph).…”

Section: Animalsmentioning

confidence: 99%

“…Cumulative mortality between ages was lower than 5% and a total of 1840 fish (EB1 = 136; EB2 = 289; EB3 = 273; EB4 = 420; EB5 = 229; EB6 = 234; EB7 = 259) sampled at both ages were considered in this study. Information about the full dataset and culture conditions was previously reported [13]. Fish were individually weighted (W) using Gram FC-200 and a photograph was taken using a Canon EOs1300D camera following the methodology previously established in PROGENSA ® [19].…”

Section: Animalsmentioning

confidence: 99%

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Genetic Estimates for Growth and Shape-Related Traits in the Flatfish Senegalese Sole

Guerrero-Cózar

Jiménez-Fernández

Berbel

et al. 2021

Animals

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Shape quality is very important in flatfish aquaculture due to the impact on commercialization. The Senegalese sole (Solea senegalensis) is a valuable flatfish with a highly elliptic body that slightly changes with age and size, and it is prone to accumulating malformations during the production cycle. The present study aims to investigate the genetic parameters of two growth traits (weight and standard length) and six shape quality predictors (ellipticity, three body heights (body height at the pectoral fin base [BHP], body maximum height [BMH] and caudal peduncle height [CPH]) and two ratios (BMH/BHP and BMH/CPH)). These traits were measured before the on-growing stage (age ~400 days (d)) and at harvest (~800 d). Phenotypic data, heritabilities and genetic and phenotypic correlations between the traits are presented and discussed. High or very high heritabilities (0.433–0.774) were found for growth traits, body heights and ellipticity and they were higher at 400 than 800 d. In contrast, the ratios of BMH/BHP and BMH/CPH were less heritable (0.144–0.306). Positive and very high (>0.95) correlations between growth traits and the three heights were found and decreased with age. In contrast, ellipticity had negative and medium-high genetic correlations with growth traits and heights, indicating fish selected for bigger size would also become rounder. The ratio of BMH/CPH showed low genetic correlations with all traits and provided complementary information to ellipticity for a better fitting to the expected lanceolate body morphology of sole. The genetic correlations for all traits at both ages were very high, indicating that selection before entering the growth-out stage in recirculation aquaculture systems is recommended to accelerate genetic gains.

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Genetic parameters for Photobacterium damselae subsp. piscicida resistance, immunological markers and body weight in gilthead seabream (Sparus aurata)

et al. 2021

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Genetic parameter estimates and identification of SNPs associated with growth traits in Senegalese sole

Cited by 9 publications

References 48 publications

Chromosome anchoring in Senegalese sole (Solea senegalensis) reveals sex-associated markers and genome rearrangements in flatfish

Chromosome anchoring in Senegalese sole (Solea senegalensis) reveals sex-associated markers and genome rearrangements in flatfish

Genetic Estimates for Growth and Shape-Related Traits in the Flatfish Senegalese Sole

Genetic parameters for Photobacterium damselae subsp. piscicida resistance, immunological markers and body weight in gilthead seabream (Sparus aurata)

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