Genetic Organization of Two Types of Flounder Warm-Temperature Acclimation-Associated 65-kDa Protein and Their Gene Expression Profiles

Kim, Young‐Ok; Park, Eun Mi; Moon, Ji Young; Nam, Bo‐Hye; Kim, Dong-Gyun; Kong, Hee Jeong; Kim, Woo Jin; Jee, Young-Ju; Lee, Sang-Jun

doi:10.1271/bbb.130263

Cited by 9 publications

(9 citation statements)

References 42 publications

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“…Several lines of evidence indicate that elevated water temperature can alter the structure or functions of gills, inducing significant morphological changes in several fish species, including reef fishes (Bowden et al, ), carp (Sollid et al, ), and goldfish (Mitrovic et al, ); these changes have been suggested to help satisfy increased oxygen demand. Moreover, it has been reported that Wap65 expression is significantly increased in flounder in hypoxic environments (Kim et al, ). Taken together, these findings suggest that the gill tissue in the Kumgang fat minnow is sensitive to temperature‐induced hypoxia, which might underlie the observed increase in kmWap65‐1 expression in this tissue.…”

Section: Discussionmentioning

confidence: 99%

“…A warm‐temperature acclimation‐associated 65‐kDa protein (Wap65) was initially identified in goldfish muscle and hepatopancreas tissue (Kikuchi et al, ), and has since been cloned from several other fish, including carp (Kinoshita et al, ), Japanese medaka (Nakaniwa et al, ), green swordtail (Aliza et al, ), European bass (Sarropoulou et al, ), pond loach (Cho et al, ), flounder (Kim et al, ), turbot (Diaz‐Rosales et al, ), and rockbream (Lee et al, ). Notably, Wap65 mRNA and plasma protein levels are highly increased by elevated water temperature.…”

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confidence: 99%

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Identification of a warm‐temperature acclimation‐associated 65‐kDa protein encoded by a temperature‐ and infection‐responsive gene in the Kumgang fat minnow Rhynchocypris kumgangensis

Kwon

Kong

et al. 2015

J. Exp. Zool.

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Water temperature is one of the most important factors in fish physiology; thus, it is important to identify genes that respond to changes in water temperature. In this study, we identified a warm- temperature acclimation-associated 65-kDa protein (Wap65) in the Kumgang fat minnow Rhynchocypris kumgangensis, a small, cold-freshwater fish species endemic to Korea. Kumgang fat minnow Wap65-1 (kmWap65-1) was cloned using polymerase chain reaction (PCR)-based strategies, and was found to be highly homologous with teleost Wap65-1 and mammalian hemopexin, a heme-binding protein that transfers plasma heme into hepatocytes. kmWap65-1 mRNA was expressed mainly in the liver and its expression levels were significantly increased by both short- and long-term exposure to high temperature, which was evaluated by real-time quantitative PCR. Furthermore, the expression levels of kmWap65-1 were highly elevated by exposure to bacterial lipopolysaccharide. These results indicate that kmWap65-1 expression is associated with environmental stresses such as increases in water temperature and bacterial infection. J. Exp. Zool. 325A:65-74, 2016. © 2015 Wiley Periodicals, Inc.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Identification of a warm‐temperature acclimation‐associated 65‐kDa protein encoded by a temperature‐ and infection‐responsive gene in the Kumgang fat minnow Rhynchocypris kumgangensis

Kwon

Kong

et al. 2015

J. Exp. Zool.

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“…1, the 10-exon structure of both Wap65 isoform genes is well conserved in most, but not all, teleostean species belonging to a wide array of taxonomic positions, suggesting that the these two isoforms evolved from a common ancestral form. However, it should also be noted that the olive flounder (Paralichthys olivaceus; Pleuronectiformes) (Kim et al, 2013) and one Cichlidae species (Maylandia zebra; Cichliformes) have only nine exons in their Wap65-1 isoform. In the olive flounder, the large exon V of the Wap65-1 isoform was believed to be a combined exon that corresponds to exons V and VI of other teleostean Wap65-1 genes (Kim et al, 2013).…”

Section: Resultsmentioning

confidence: 99%

“…However, it should also be noted that the olive flounder (Paralichthys olivaceus; Pleuronectiformes) (Kim et al, 2013) and one Cichlidae species (Maylandia zebra; Cichliformes) have only nine exons in their Wap65-1 isoform. In the olive flounder, the large exon V of the Wap65-1 isoform was believed to be a combined exon that corresponds to exons V and VI of other teleostean Wap65-1 genes (Kim et al, 2013). In the case of M. zebra, the exon-shuffling pattern is unclear due to unfinished sequence information on the Wap65 loci.…”

Section: Resultsmentioning

confidence: 99%

Genomic Organization and Isoform-Dependent Expression Patterns of Wap65 genes in Various Tissues during Immune Challenges in the Mud Loach Misgurnus mizolepis

Kim¹,

Cho²,

Lee³

et al. 2014

Fisheries and aquatic sciences

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Genomic organization, including the structural characteristics of 5´-flanking regions of two 65-kDa protein (WAP65) isoform genes associated with warm temperature acclimation, were characterized and their transcriptional responses to immune challenges were examined in the intestine, kidney and spleen of the mud loach (Misgurnus mizolepis; Cypriniformes). Both mud loach Wap65 isoform genes displayed a 10-exon structure that is common to most teleostean Wap65 genes. The two mud loach Wap65 isoforms were predicted to possess various stress-and immune-related transcription factor binding sites in their regulatory regions; however, the predicted motif profiles differed between the two isoforms, and the inflammation-related transcription factor binding motifs, such as NF-κB and CREBP sites, were more highlighted in the Wap65-2 isoform than the Wap65-1 isoform. The results of qRT-PCR indicated that experimental immune challenges using Edwardsiella tarda, lipopolysaccharide or polyI:C induced the Wap65-2 isoform more than Wap65-1 isoform, although modulation patterns in response to these challenges were tissue-and stimulant-dependent. This study confirms that functional diversification between the two mud loach Wap65 isoforms (i.e., closer involvement of Wap65-2 in the acute phase of inflammation and innate immunity) occurs at the mRNA level in multiple tissues, and suggests that such differential modulation patterns between the two isoforms are related to the different transcription factor binding profiles in their regulatory regions.

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“…It is similar to mammalian hemopexin, which scavenges heme to protect from oxidative damage, limit pathogen access to heme, and maintain iron homeostasis . In addition, WAP65s are involved in innate immunity, stress response and teleost development . However, the profiles of wap65 genes after stimulatory treatments differ, due to species‐ and/or lineage‐specific evolution , requiring further study of their physiological roles.…”

Section: Discussionmentioning

confidence: 99%

Proteomic analysis through larval development of Solea senegalensis flatfish

et al. 2015

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The post-embryonic development of the Senegalese sole, Solea senegalensis, a flatfish of growing interest in fisheries and aquaculture, is associated with drastic morpho-physiological changes during metamorphosis. Although in the last two decades knowledge on sole culture has notably increased, especially in Southern Europe, its progress was restricted due to lack of methods to control reproduction, improve larval quality and increase juvenile disease resistance. A limited knowledge of the physiological, molecular and genetic mechanisms involved is at the base of such limitation. A proteomic study was carried out to explore the molecular events that occur during S. senegalensis ontogenesis. Protein expression changes were monitored in larvae from 5 to 21 dph by combining 2DE and protein identification with de novo MS/MS sequencing. An average of 6177 ± 282 spots was resolved in 2DE gels. Hierarchical cluster analysis of the 705 selected spots grouped them in eight patterns. Thirty-four proteins were identified and assigned biological functions including structure, metabolism highlighting energy metabolism, transport, protein folding, stress response, chromatin organization and regulation of gene expression. These changes provide a sequential description of the molecular events associated with the biochemical and biological transformations that occur during sole larval development.

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Genetic Organization of Two Types of Flounder Warm-Temperature Acclimation-Associated 65-kDa Protein and Their Gene Expression Profiles

Cited by 9 publications

References 42 publications

Identification of a warm‐temperature acclimation‐associated 65‐kDa protein encoded by a temperature‐ and infection‐responsive gene in the Kumgang fat minnow Rhynchocypris kumgangensis

Identification of a warm‐temperature acclimation‐associated 65‐kDa protein encoded by a temperature‐ and infection‐responsive gene in the Kumgang fat minnow Rhynchocypris kumgangensis

Genomic Organization and Isoform-Dependent Expression Patterns of Wap65 genes in Various Tissues during Immune Challenges in the Mud Loach Misgurnus mizolepis

Proteomic analysis through larval development of Solea senegalensis flatfish

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