Genetic Organization of Polytene Chromosomes

Жимулев, И. Ф.

doi:10.1016/s0065-2660(08)60476-9

Cited by 57 publications

(54 citation statements)

References 2,033 publications

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“…To classify a puff stage (PS) correctly, we used a detailed schedule of puff changes in ontogenesis, which was established earlier (reviewed in Zhimulev 1999) and is routinely used for this purpose.…”

Section: Methodsmentioning

confidence: 99%

The Drosophila Dosage Compensation Complex Binds to Polytene Chromosomes Independently of Developmental Changes in Transcription

et al. 2006

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In Drosophila, the dosage compensation complex (DCC) mediates upregulation of transcription from the single male X chromosome. Despite coating the polytene male X, the DCC pattern looks discontinuous and probably reflects DCC dynamic associations with genes active at a given moment of development in a salivary gland. To test this hypothesis, we compared binding patterns of the DCC and of the elongating form of RNA polymerase II (PolIIo). We found that, unlike PolIIo, the DCC demonstrates a stable banded pattern throughout larval development and escapes binding to a subset of transcriptionally active areas, including developmental puffs. Moreover, these proteins are not completely colocalized at the electron microscopy level. These data combined imply that simple recognition of PolII machinery or of general features of active chromatin is either insufficient or not involved in DCC recruitment to its targets. We propose that DCC-mediated site-specific upregulation of transcription is not the fate of all active X-linked genes in males. Additionally, we found that DCC subunit MLE associates dynamically with developmental and heat-shock-induced puffs and, surprisingly, with those developing within DCC-devoid regions of the male X, thus resembling the PolIIo pattern. These data imply that, independently of other MSL proteins, the RNA-helicase MLE might participate in general transcriptional regulation or RNA processing.

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Section: Methodsmentioning

confidence: 99%

The Drosophila Dosage Compensation Complex Binds to Polytene Chromosomes Independently of Developmental Changes in Transcription

et al. 2006

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“…In polytene chromosomes, gene rich regions are located in more loosely packed and more lightly staining interbands, whereas more densely condensed chromatin (heterochromatin) is localized either to compacted bands or highly condensed lower copy centromeric heterochromatin (reviewed in ref. 34). This localization to the comparatively decondensed interbands was also detected with the fluorescent signal from the TDtomato::L(3)mbt transgene we constructed, shown in Fig.…”

Section: L(3)mbt Localization On Polytene Chromosomes and Correlationmentioning

confidence: 99%

Chromatin reader L(3)mbt requires the Myb–MuvB/DREAM transcriptional regulatory complex for chromosomal recruitment

Blanchard

Georlette

Antoszewski

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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Lethal malignant brain tumors (lmbt) result from the loss of the conserved transcriptional repressor l(3)mbt, in Drosophila melanogaster. Similar mutations in the human homolog L3MBTL1 correlate with some cancers. The protein's C-terminal MBT repeats bind mono and dimethylated histones in vitro, which could influence recruitment of L3MBTL1 to its target sites. The L(3)mbt chromatin targeting mechanism, however, is controversial and several studies suggest insufficiency or a minor role for histone methylation in determining the site specificity for recruitment. We report that L(3)mbt colocalizes with core members of the Myb-MuvB/DREAM (MMB/DREAM) transcriptional regulatory complex genome-wide, and that L(3)mbt-mediated repression requires this complex in salivary glands and larval brains. Loss of l(3)mbt or of MMB components through mutation cause similar spurious expression of genes, including the transposon regulatory gene piwi, in terminally differentiated cells. The DNA-binding MMB core component Mip120 (Lin54) is required for L(3)mbt recruitment to chromosomes, whereas Mip130 (Lin9) (an MMB core protein) and E2f2 (an MMB transcriptional repressor) are not, but are essential for repression. Cytolocalization experiments suggest the presence of sitespecific differential composition of MMB in polytene chromosomes where some loci were bound by a Myb-containing or alternatively, an E2f2 and L(3)mbt form of the complex.epigenetics | bar coding | tumorigenesis

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“…4) -a stage normally observed only early in the last larval instar, in chromosomes too small to be suitable for cytogenetic analysis (Zhimulev and Belyaeva, 1999). PS1′ is characterized by small puffs at sites encoding glue proteins (3C8-12, 68C, 90D), at other sites that normally regress in the presence of ecdysone (42A4-18, 43E, 53C, 56E1-2), at several sites not correlated with development (47A9-16, 58E, 67B, 71CE, 72D, 88D, 88E) and at the PS1′-specific sites 78E and 80A3 (Becker, 1959, Ashburner, 1967Ashburner and Berendes, 1978;Zhimulev, 1999). In PS1′, there is no puff at 85F1-6, a region which puffs continuously during the subsequent stages PS1 to PS11.…”

Section: Sgs3mentioning

confidence: 99%

EcR isoforms inDrosophila: testing tissue-specific requirements by targeted blockade and rescue

et al. 2003

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The three Drosophila EcR isoforms differ only at their N termini;thus, they share the conserved ligand-binding domain transcriptional activation function (AF2) and only differ in the unconserved A/B region, which contains a second, isoform-specific, activation function (AF1). We have developed a dominant-negative mutant EcR (EcR-DN), expressed it in flies with the GAL4/UAS system, and used it to block ecdysone signaling in eight tissues or groups of tissues. Localized EcR-DN arrests ecdysone-dependent development in the target cells and often — because of a molting checkpoint —arrests development globally. Simultaneously expressing individual wild-type EcR isoforms in the same target tissues suppresses the EcR-DN phenotype and identifies the rescuing isoform as sufficient to support the development of the target. Every isoform, and even an N-terminal truncated EcR that lacks any AF1, supports development in the fat body, eye discs, salivary glands,EH-secreting neurosecretory cells and in the dpp expression domain,implying that AF1 is dispensable in these tissues. By contrast, only EcR-A is able to support development in the margins of the wing discs, and only EcR-B2 can do so in the larval epidermis and the border cells of the developing egg chamber. In light of our results, the simplest explanations for the widespread spatial and temporal variations in EcR isoform titers appear untenable.

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Genetic Organization of Polytene Chromosomes

Cited by 57 publications

References 2,033 publications

The Drosophila Dosage Compensation Complex Binds to Polytene Chromosomes Independently of Developmental Changes in Transcription

The Drosophila Dosage Compensation Complex Binds to Polytene Chromosomes Independently of Developmental Changes in Transcription

Chromatin reader L(3)mbt requires the Myb–MuvB/DREAM transcriptional regulatory complex for chromosomal recruitment

EcR isoforms inDrosophila: testing tissue-specific requirements by targeted blockade and rescue

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