Genetic networks responsive to low-intensity pulsed ultrasound in human lymphoma U937 cells

Tabuchi, Yoshiaki; Takasaki, Ichiro; Zhao, Qing‐Li; Wada, Shigehito; Hori, Takeshi; Feril, Loreto B.; Tachibana, Katsuro; Nomura, Takaharu; Kondo, Takashi

doi:10.1016/j.canlet.2008.05.018

Cited by 41 publications

(37 citation statements)

References 46 publications

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“…This setup allowed for standing wave formation due to the reflection of ultrasound radiation at the water-air interface (14). We used a near acoustic field produced by the liquid-air interface and did not reduce standing waves because of the efficient occurrence of cavitation (15).…”

Section: Methodsmentioning

confidence: 99%

The improvement of liposome-mediated transfection of pEGFP DNA into human prostate cancer cells by combining low-frequency and low-energy ultrasound with microbubbles

Bai

Wu²,

Shen³

et al. 2011

Oncol Rep

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Abstract. The aim of this study was to explore the use of a contrast agent to study the effects of exposure to ultrasound, in combination with microbubbles, on liposome-mediated transfection of genes into human prostate cancer cells. A contrast agent was used to study the effects of ultrasound exposure in combination with microbubbles on liposomes, which transfect genes into human prostate cancer cells. The human prostate cancer cell line PC-3 in suspension was exposed to ultrasound with a 20% duty cycle (i.e., 2 sec 'on' time and 8 sec 'off' time) lasting 5 min, with and without ultrasound contrast agent (SonoVue™) using a digital sonifier at a frequency of 21 kHz and an intensity of 4.6 mW/cm 2 . Immediately after exposure to ultrasound, cell viability and membrane damage were measured. After exposure to ultrasound, the cell suspensions were put into 12-well plates and cultured for 24 h. Fluorescence microscopy and flow cytometry were used to detect pEGFP transfection efficiency. Exposure to ultrasound alone and ultrasound combined with microbubbles resulted in minimal cell death and induced negligible cell membrane damage. Ultrasound combined with microbubbles had a greater effect on cell membrane damage in all groups: the average cell membrane damage was 41.87%, and it was approximately 42-fold greater than in the control group. The average transfection efficiency of PC-3 cells was 20.30% for the liposome (Lipofectamine™)+pEGFP+ultrasound+ultrasound contrast agent (SonoVue) group; this was the highest rate of all groups measured and was approximately 81-fold greater than that of the control group. The use of low-frequency and low-energy ultrasound, in combination with microbubbles, could be a potent physical method for increasing liposome gene delivery efficiency. This technique is a promising non-viral approach that can be used in prostate cancer gene therapy.

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Section: Methodsmentioning

confidence: 99%

The improvement of liposome-mediated transfection of pEGFP DNA into human prostate cancer cells by combining low-frequency and low-energy ultrasound with microbubbles

Bai

Wu²,

Shen³

et al. 2011

Oncol Rep

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“…At present, the majority of studies investigate the effect of low-frequency ultrasound on the biological behavior of tumor cells at the genetic level. Tabuchi et al (23) observed that 193 genes were downregulated and 201 were upregulated in tumor cells following irradiation by low-frequency ultrasound. These genes are associated with cell growth, proliferation, apoptosis, movement, polymorphism and death (24).…”

Section: Discussionmentioning

confidence: 99%

Upregulation of Beclin-1 expression in DU-145 cells following low-frequency ultrasound irradiation combined with microbubbles

et al. 2015

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Abstract. Castration-resistant prostate cancer (PCa) is difficult to treat. Autophagy, which is an evolutionarily conserved mechanism, plays an important role in cancer development. The balance between cell death and survival in different stages varies in cancer development. The role of autophagy in PCa development has not yet been fully elucidated. Ultrasound may be of value in the treatment of PCa. The aim of the present study was to investigate the association between autophagy and ultrasound combined with microbubbles. The MTT assay was used to evaluate cell viability. Autophagy was observed by transmission electron microscopy. Reverse transcription-polymerase chain reaction and western blot analysis were used to assess the expression of autophagy-related genes. The results revealed that cell viability was significantly reduced by ultrasound combined with microbubbles in DU145 PCa cells. The present study demonstrated that ultrasound combined with microbubbles induced autophagy and autophagy-related DU-145 cell death. Notably, these findings highlighted additional mechanisms that suggest the potential of ultrasound-modulated autophagy as a novel therapeutic strategy for PCa.

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“…Tabuchi, et al [10,11] reported two gene networks response to ultrasound exposure and indicated the potential signaling pathway. However, compared to the genes, proteins are the real main participators involved in the different metabolic processes, both in the tumor pathogenesis, growth, metastasis, and after stimulation by physical and chemical factors [12].…”

Section: Introductionmentioning

confidence: 99%

Potential mechanism of apoptosis induced by ultrasound in human hepatocarcinoma cells via comparative proteomic analysis

Feng

Wan

2015

BME

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Abstract. To analyze the potential molecular mechanism of ultrasound induced apoptosis in cancer cells, comparative proteomic methods were introduced in the study. After ultrasound exposure at the intensity of 1.2 W/cm 2 , the human SMMC-7721 hepatocarcinoma cells were stained by trypan blue to detect the morphologic changes, and then the flow cytometry was used to examine the percentage of early apoptosis via double staining of FITC-labelled Annexin V and Propidium iodide. The proteins were separated by two-dimensional (2D) SDS polyacrylamide gel electrophoresis (PAGE). Among them, the differently expressed proteins were identified by MALDI-TOF mass spectrometry to reveal the key proteins response to ultrasound exposure. It's proved early apoptosis of cells were induced by focused ultrasound. After ultrasound exposure, the expressing characteristics of several proteins changed, in which some proteins in HSP family are associated with apoptosis initiation. It is suggested that the focused ultrasound could be applied in the assistant cancer therapy. Moreover, it is proved the comparative proteomic methods could supply information about the protein expression to analyze the metabolic processes related to bio-effects of biomedical ultrasound.

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Genetic networks responsive to low-intensity pulsed ultrasound in human lymphoma U937 cells

Cited by 41 publications

References 46 publications

The improvement of liposome-mediated transfection of pEGFP DNA into human prostate cancer cells by combining low-frequency and low-energy ultrasound with microbubbles

The improvement of liposome-mediated transfection of pEGFP DNA into human prostate cancer cells by combining low-frequency and low-energy ultrasound with microbubbles

Upregulation of Beclin-1 expression in DU-145 cells following low-frequency ultrasound irradiation combined with microbubbles

Potential mechanism of apoptosis induced by ultrasound in human hepatocarcinoma cells via comparative proteomic analysis

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