Genetic Mouse Models for Osteoarthritis Research

Shen, Jie; Wang, Meina; Jin, Hongting; Sampson, Erik R.; Chen, Di

doi:10.5772/27835

Cited by 2 publications

(1 citation statement)

References 95 publications

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“…Chondrocytes are the only permanent residence at articular cartilage ( 12 ). It produces many major and minor components of articular cartilage, such as: collagen type II, glicosaminoglican and hialuronan, woven around condrosit as a major component ( 13 ),( 14 ), and collagen type V, VI, IX, X, XI, XII, XIV, decorin, biglican, fibromodulin, perlecan; non colagenus protein: matrilins, trombospondin-5/ COMP ( 15 ),( 16 ),( 17 ). All of the components above need to support matrix integrity, if chondrocytes distance was increased by the influence of hyperglycemia at animal model, it increases risk to lowering of matrix density, which can decrease intracellular matrix strain fields by the dynamical movement of the joint ( 18 ).…”

Section: Discussionmentioning

confidence: 99%

Chondrocyte Intracellular Matrix Strain Fields of Articular Cartilage Surface in Hyperglycemia Model of Rat: Cellular Morphological Study

et al. 2018

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Introduction:Chondrocyte is one cell in articular cartilage was products many proteins, molecules, and other factors. The external influence of cartilage, such as: hyperglycemia was entering joint capsule and impact to the chondrocytes and the cartilage. Hyperglycemia caused modification of heparan sulfate proteoglycan 2 (perlecan) proteins through glycation process.Aim:The aim of this study was to analyze morphological changing of chondrocytes pericellular matrix by the influence of hyperglycemia.Material and Methods:Eighteen adult male rats were divided into six groups: control, rat treated with sugar intake was 0.5 mg/kg, 0.75 mg/ kg, 1mg/kg, 1.5 g/kg and 2 mg/kg of body weight. The animal model was dislocated and left knee was taken to observe changing of chondrocytes pericellular matrix strain fields by Scanning Electron Microscope (SEM) from perpendicular to femoral condylus cartilage.Results:Changing of chondrocytes intracellular matrix strain fields as changing of cell diameters and cell distances at group control and group I to group V, which cell diameters was lower level and cell distances was the highest level at over diet 2. This changing of intracellular matrix strain fields was corresponding to changing chondrocytes morphology in hyperglycemia condition, due to hypertrophic stage as adaptive responses. This research as based on next research for accomplish of hyperglycemia influence to morphology articular cartilage changing to prevent degeneration of cartilage towards osteoarthritis.Conclusions:Present study concludes that hyperglycemia influence to chondrocyte intracellular matrix strain fields changing.

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