Genetic, molecular, and functional analysis of Streptococcus faecalis R plasmid pJH1

Banai, M; LeBlanc, Donald J.

doi:10.1128/jb.155.3.1094-1104.1983

Cited by 15 publications

(5 citation statements)

References 24 publications

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“…The results presented in this report demonstrate that a 5.1kb segment of DNA from pJH1 carrying an Emr determinant can insert into at least four different locations on pJH2, a Hly-Bcn plasmid that shares no detectable DNA sequence homology with pJH1 (1,10). This segment of DNA mediates inducible resistance to Em (data not shown) and contains four AvaI restriction endonuclease cleavage sites.…”

mentioning

confidence: 70%

“…1B; lane 4) hybridized to the probe. This DNA sequence homology was not from the pJH2 EcoRI A fragment component of the probe since there is no homology between pJH2 and pJH1 (1,10) or pAD2 (unpublished observation). Tn917 contains four AvaI restriction endonuclease cleavage sites (3).…”

mentioning

confidence: 94%

“…faecalis JH1 also contains two plasmids. pJH1 is an 80.7-kb R plasmid, and pJH2 is a 58-kb Hly-Bcn plasmid (1,7,8,10). Either plasmid can be transferred by conjugation in broth culture.…”

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confidence: 99%

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Streptococcus faecalis R plasmid pJH1 contains an erythromycin resistance transposon (Tn3871) similar to transposon Tn917

Banai¹,

LeBlanc²

1984

J Bacteriol

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confidence: 70%

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confidence: 94%

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Streptococcus faecalis R plasmid pJH1 contains an erythromycin resistance transposon (Tn3871) similar to transposon Tn917

Banai¹,

LeBlanc²

1984

J Bacteriol

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“…Genetic and plasmid variations may change the host virulence and specificity. As diverse genetic sources, plasmids have been found to carry genes for drug resistance and virulence in various streptococcal species [ 21 – 23 ]. Furthermore, GBS virulence to fish also depends on environmental conditions, such as temperature above 26 °C increases the GBS virulence to tilapia [ 24 ].…”

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confidence: 99%

Genetic and pathogenic difference between Streptococcus agalactiae serotype Ia fish and human isolates

et al. 2016

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BackgroundStreptococcus agalactiae (GBS) is a common pathogen to infect newborn, woman, the elderly, and immuno-compromised human and fish. 37 fish isolates and 554 human isolates of the GBS in 2007–2012 were investigated in serotypes, antibiotic susceptibility, genetic difference and pathogenicity to tilapia.ResultsPCR serotyping determined serotype Ia for all fish GBS isolates and only in 3.2 % (3–4.2 %) human isolates. For fish isolates, all consisted a plasmid less than 6 kb and belonged to ST7 type, which includes mainly pulsotypes I and Ia, with a difference in a deletion at the largest DNA fragment. These fish isolates were susceptible to all antimicrobials tested in 2007 and increased in non-susceptibility to penicillin, and resistance to clindamycin and ceftriaxone in 2011. Differing in pulsotype and lacking plasmid from fish isolates, human serotype Ia isolates were separated into eight pulsotypes II–IX. Main clone ST23 included pulsotypes II and IIa (50 %) and ST483 consisted of pulsotype III. Human serotype Ia isolates were all susceptible to ceftriaxone and penicillin and few were resistant to erythromycin, azithromycin, clindamycin, levofloxacin and moxifloxacine with the resistant rate of 20 % or less. Using tilapia to analyze the pathogenesis, fish isolates could cause more severe symptoms, including hemorrhage of the pectoral fin, hemorrhage of the gill, and viscous black and common scites, and mortality (>95 % for pulsotype I) than the human isolates (<30 %); however, the fish pulostype Ia isolate 912 with deletion caused less symptoms and the lowest mortality (<50 %) than pulsotype I isolates.ConclusionGenetic, pathogenic, and antimicrobial differences demonstrate diverse origin of human and fish serotype Ia isolates. The pulsotype Ia of fish serotype Ia isolates may be used as vaccine strains to prevent the GBS infection in fish.Electronic supplementary materialThe online version of this article (doi:10.1186/s12866-016-0794-4) contains supplementary material, which is available to authorized users.

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“…homology between pAMal and pJH1 appears to extend beyond the respective tetracycline resistance determinants of the two plasmids (2).…”

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confidence: 96%

Streptococcus faecalis R plasmid pJH1 contains a pAM alpha 1 delta 1-like replicon

Banai¹,

Gonda²,

Ranhand³

et al. 1985

J Bacteriol

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Streptococcus faecalis R plasmid pJHl did not transform competent strains of Streptococcus sanguis. A hybrid plasmid, pDL310, consisting of virtually all of the S. faecalis hemolysin-bacteriocin plasmid pJH2 and a segment of pJHl DNA that included the tetracycline resistance determinant, yielded tetracycline-resistant transformants at a frequency of less than 10-8 transformants per CFU, when it was added to a competent culture of S. sanguis Wicky. Four of the transformants contained a 4.7-kllobase plasmid (pDL316) that transformed strain Wicky at a frequency of 8.6 x 10-8. Restriction endonuclease digests, agarose gel electrophoresis, and Southern blot hybridizations indicated that pDL316 consisted entirely of pJHl-derived DNA. Additional restriction studies, Southern blot hybridizations, and heteroduplex analyses indicated that pDL316 was very closely related to 4.6-kilobase tetracycline resistance plasmid pAMa1Aj, a derivative of 9.0-kilobase S. faecalis plasmid pAMalpha 1.Conjugative multiple-antibiotic-resistance plasmid pJH1 from Streptococcus faecalis var. zymogenes strain JH1 (10) mediates resistance to kanamycin, streptomycin, tetracycline, and the MLS group of antibiotics (macrolides, lincosamides, and the streptogramin B type of antibiotics). Recently, we constructed a detailed restriction endonuclease map of pJH1 and located the kanamycin, streptomycin, and MLS resistance determinants within a 15-kilobase (kb) EcoRI fragment on the map of this 80 kb molecule (2). The MLS resistance determinant of pJH1 was shown to be 'on transposon Tn3871 (3), which is very similar, if not identical, to Tn917, an MLS resistance transposon originally found on pAD2, an R plasmid from S. faecalis DS16 (7,26).The tetracycline resistance determinant of pJH1, which is located several kilobases away from the kanamycin, streptomycin, and MLS resistance determinants, has been shown to share DNA sequence homology with the corresponding determinant of pAMalpha 1 (2, 4). This latter plasmid is a 9-kb nonconjugative tetracycline resistance plasmid originally described in S. faecalis DS5 (5). Furthermore, the on July 16, 2020 by guest

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Genetic, molecular, and functional analysis of Streptococcus faecalis R plasmid pJH1

Cited by 15 publications

References 24 publications

Streptococcus faecalis R plasmid pJH1 contains an erythromycin resistance transposon (Tn3871) similar to transposon Tn917

Streptococcus faecalis R plasmid pJH1 contains an erythromycin resistance transposon (Tn3871) similar to transposon Tn917

Genetic and pathogenic difference between Streptococcus agalactiae serotype Ia fish and human isolates

Streptococcus faecalis R plasmid pJH1 contains a pAM alpha 1 delta 1-like replicon

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