Genetic Modifications of Corn

Cabrera-Ponce, José Luis; Valencia-Lozano, Eliana; Trejo-Saavedra, Diana Lilia

doi:10.1016/b978-0-12-811971-6.00003-6

Cited by 8 publications

(4 citation statements)

References 230 publications

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“…Moreover, the Barnase-barstar system facilitates the production of hybrid seeds on a commercial scale, overcoming logistical challenges associated with manual emasculation and pollination. With male sterility conferred by the Barnase gene, hybrid seed production becomes more efficient and cost-effective, enabling breeders to meet the growing demand for highquality mustard seeds in agricultural markets [30]. The adoption of the Barnase-barstar gene system underscores the pivotal role of biotechnology in addressing the evolving needs of modern agriculture and ensuring food security.…”

Section: Barnase-barstar Gene Systemmentioning

confidence: 99%

Genetic Engineering in Indian Mustard (Brassica juncea L.): Current Progress and Future Directions for Enhanced Crop Improvement

Gupta,

Kumar,

Janeja

et al. 2024

JABB

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Genetic tools have revolutionized mustard improvement strategies, offering innovative avenues to enhance its agronomic traits and quality parameters. CRISPR technology, exemplified by research at the National Institute of Plant Genome Research in India, has enabled precise manipulation of glucosinolate levels in mustard plants, improving mustard oil quality. The Barnase-barstar gene system has facilitated the development of mustard hybrids, increasing yield and resilience. Additionally, Microsatellites have emerged as indispensable tools for understanding genetic relationships within mustard populations. These genetic tools hold promise for addressing agronomic challenges and meeting market demands in mustard cultivation. However, their deployment raises ethical, regulatory, and socio-economic considerations that require careful consideration. Responsible stewardship and transparent deployment of these technologies are essential to realize their full potential in enhancing mustard crops and ensuring a sustainable future for food production. In conclusion, genetic engineering offers exciting avenues for mustard improvement, with CRISPR, the Barnase-barstar gene system, and Microsatellites playing pivotal roles in enhancing crop quality, yield potential, and resilience. As mustard continues to play a crucial role in global agriculture and food security, the responsible utilization of these genetic tools holds promise for meeting the evolving needs of farmers and consumers worldwide. Furthermore, the paper briefly discusses the application of these genetic tools in enhancing Dhara Mustard, a popular variety in Indian agriculture, emphasizing its potential impact on addressing agricultural challenges and meeting consumer demands.

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Section: Barnase-barstar Gene Systemmentioning

confidence: 99%

Genetic Engineering in Indian Mustard (Brassica juncea L.): Current Progress and Future Directions for Enhanced Crop Improvement

Gupta,

Kumar,

Janeja

et al. 2024

JABB

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“…The major components of CBB integrated pest management (IPM) strategies used in most coffee-growing regions around the world include: monitoring of populations (traps and tree sampling), cultural control (preharvest and post-harvest sanitation), biological control (use of natural enemies), chemical control (use of insecticides and repellents), and physical control (exclusion netting and border crops) (Cabrera-Ponce et al, 2019).…”

Section: Transgenic Coffee Fruits Resistant To Cbbmentioning

confidence: 99%

Coffea arabica L. Resistant to Coffee Berry Borer (Hypothenemus hampei) Mediated by Expression of the Bacillus thuringiensis Cry10Aa Protein

et al. 2021

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Coffea spp. are tropical plants used for brewing beverages from roasted and grounded seeds, the favorite drink in the world. It is the most important commercial crop plant and the second most valuable international commodity after oil. Global coffee trade relies on two Coffea species: C. arabica L. (arabica coffee) comprising 60% and C. canephora (robusta) comprising the remaining 40%. Arabica coffee has lower productivity and better market price than robusta. Arabica coffee is threatened by disease (i.e., coffee leaf rust), pests [i.e., Hypothenemus hampei or coffee berry borer (CBB) and nematodes], and susceptibility to climate change (i.e., drought and aluminum toxicity). Plant biotechnology by means of tissue culture inducing somatic embryogenesis (SE) process, genetic transformation, and genome editing are tools that can help to solve, at least partially, these problems. This work is the continuation of a protocol developed for stable genetic transformation and successful plant regeneration of arabica coffee trees expressing the Bacillus thuringiensis (Bt) toxin Cry10Aa to induce CBB resistance. A highly SE line with a high rate of cell division and conversion to plants with 8-month plant regeneration period was produced. To validate this capability, gene expression analysis of master regulators of SE, such as BABY BOOM (BBM), FUS3, and LEC1, embryo development, such as EMB2757, and cell cycle progression, such as ETG1 and MCM4, were analyzed during induction and propagation of non-competent and highly competent embryogenic lines. The particle bombardment technique was used to generate stable transgenic lines after 3 months under selection using hygromycin as selectable marker, and 1 month in plant regeneration. Transgenic trees developed fruits after 2 years and demonstrated expression of the Bt toxin ranging from 3.25 to 13.88 μg/g fresh tissue. Bioassays with transgenic fruits on CBB first instar larvae and adults induced mortalities between 85 and 100% after 10 days. In addition, transgenic fruits showed a seed damage lower than 9% compared to 100% of control fruits and adult mortality. This is the first report on stable transformation and expression of the Cry10Aa protein in coffee plants with the potential to control CBB.

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“…Currently, more than 800 sequences of Cry proteins are registered, which are grouped into 78 different classes and are specifically active mostly against some insects and nematodes [12]. The genes encoding the insecticidal proteins in some Bt microbial products have been successfully cloned, integrated, and expressed in genetically modified plants [13,14,15,16,17,18,19] to confer resistance against insect damage. Bt-protected crops such as corn, cotton, soybean, and potato have demonstrated significant benefits since their introduction in 1996.…”

Section: Introductionmentioning

confidence: 99%

Development of an Efficient Protocol to Obtain Transgenic Coffee, Coffea arabica L., Expressing the Cry10Aa Toxin of Bacillus thuringiensis

Valencia-Lozano

Cabrera-Ponce

Gómez-Lim

et al. 2019

IJMS

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This report presents an efficient protocol of the stable genetic transformation of coffee plants expressing the Cry10Aa protein of Bacillus thuringiensis. Embryogenic cell lines with a high potential of propagation, somatic embryo maturation, and germination were used. Gene expression analysis of cytokinin signaling, homedomains, auxin responsive factor, and the master regulators of somatic embryogenesis genes involved in somatic embryo maturation were evaluated. Plasmid pMDC85 containing the cry10Aa gene was introduced into a Typica cultivar of C. arabica L. by biobalistic transformation. Transformation efficiency of 16.7% was achieved, according to the number of embryogenic aggregates and transgenic lines developed. Stable transformation was proven by hygromycin-resistant embryogenic lines, green fluorescent protein (GFP) expression, quantitative analyses of Cry10Aa by mass spectrometry, Western blot, ELISA, and Southern blot analyses. Cry10Aa showed variable expression levels in somatic embryos and the leaf tissue of transgenic plants, ranging from 76% to 90% of coverage of the protein by mass spectrometry and from 3.25 to 13.88 μg/g fresh tissue, with ELISA. qPCR-based 2−ΔΔCt trials revealed high transcription levels of cry10Aa in somatic embryos and leaf tissue. This is the first report about the stable transformation and expression of the Cry10Aa protein in coffee plants with the potential for controlling the coffee berry borer.

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Genetic Modifications of Corn

Cited by 8 publications

References 230 publications

Genetic Engineering in Indian Mustard (Brassica juncea L.): Current Progress and Future Directions for Enhanced Crop Improvement

Genetic Engineering in Indian Mustard (Brassica juncea L.): Current Progress and Future Directions for Enhanced Crop Improvement

Coffea arabica L. Resistant to Coffee Berry Borer (Hypothenemus hampei) Mediated by Expression of the Bacillus thuringiensis Cry10Aa Protein

Development of an Efficient Protocol to Obtain Transgenic Coffee, Coffea arabica L., Expressing the Cry10Aa Toxin of Bacillus thuringiensis

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