Genetic Modification of Pest and Beneficial Insects for Pest-Management Programs

Mosquito Trilogy: Microbiota, Immunity and Pathogens, and Their Implications for the Control of Disease Transmission

“…In arthropods, paratransgenesis is based on the genetic manipulation of symbionts for the production of effector molecules (e.g., antipathogens or immunomodulatory), followed by the re-introduction of the modified symbiont into the arthropod host, to reduce its vector competence (Ogaugwu and Durvasula, 2017;Wang and Jacobs-Lorena, 2017;Gao H. et al, 2020; Figure 2). The choice of a good candidate symbiont is crucial (Hoy, 2013). First, the symbiont should be stably associated with the insect vector, efficiently transmitted vertically and/or horizontally, and persist long enough to produce the effector molecules (Wilke and Marrelli, 2015).…”

A simplified strategy to package foreign proteins into baculovirus occlusion bodies without engineering the viral genome

“…Formulation of biopesticides based on wild type baculoviruses has been widely tested and approved for field use by regulatory authorities in many countries. Even though recombinant baculoviruses were reported to increase the speed of kill or reduce the lethal doses needed to control of the target pest (Kroemer et al, 2015;Yang et al, 2017), extensive J o u r n a l P r e -p r o o f efforts should be made to determine the environmental impact (Haase et al, 2015;Hoy, 2019;Tiedje et al, 2014). Regulations preventing the release of genetically modified viruses on a broad scale encouraged us to develop a proof of concept to test the ability of a transformed cell line, UFLAg-GFP::PEPAg to express and deliver the fusion polypeptide GFP::PEPAg to different wild type OBs (Fig.…”