Genetic Modification and Sequence Analysis of Probiotic Microorganisms

Akçelik, Mustafa; Akçelik, Nefise; Şanlibaba, Pınar; Tezel, Başar Uymaz

doi:10.1016/b978-0-12-822909-5.00006-x

Cited by 1 publication

(14 citation statements)

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“…The use of genetic engineering to create probiotic microorganisms with specified properties is carried out by manipulating their own DNA and the DNA of other microorganisms (that also have GRAS status) via the following methods: induced mutagenesis (random mutagenesis); using vectors obtained from cryptic LAB plasmids or bifidobacteria; integration of target genes into the probiotic strain using site-specific recombination; using the CRISPR-Cas system [85].…”

Section: Methods Of Modifying the Genome Of Probioticsmentioning

confidence: 99%

“…It can be said that LAB were the first microorganisms to be modified by such vectors. The name of these vectors is cryptic plasmidan extrachromosomal DNA element in the form of a doublestranded DNA molecule closed in a ring [85,86].…”

Section: Using Cryptic Plasmids To Create Recombinant Probioticsmentioning

confidence: 99%

“…LAB have a large number of cryptic plasmids that are small in size and can show high resistance to in vitro manipulations. In addition, they contain genes necessary for plasmid replication and mobilization, and have no apparent effect on the host phenotype [85]. At this time, many critical LAB plasmids were isolated and used to improve LAB properties [86].…”

Section: Using Cryptic Plasmids To Create Recombinant Probioticsmentioning

confidence: 99%

“…lactis (pWV01, pSH71, pD125, pLC2.1], Str. thermophilus (pA2, pA33, pSt04, pJ34, LeJ2, p4028, pRS1) [85,86]. A large number of plasmids were also isolated from other probiotic genera, e.g., Lactobacillus, Pediococcus, and Bifidobacterium [85].…”

Section: Using Cryptic Plasmids To Create Recombinant Probioticsmentioning

confidence: 99%

“…thermophilus (pA2, pA33, pSt04, pJ34, LeJ2, p4028, pRS1) [85,86]. A large number of plasmids were also isolated from other probiotic genera, e.g., Lactobacillus, Pediococcus, and Bifidobacterium [85]. In general, the use of cryptic plasmids improves some properties of LAB, such as: resistance to bacteriophages, resistance to bacteriocins (e.g., nisin), acquisition of resistance to heavy metals (e.g., cadmium, copper), utilization of other carbon sources (e.g., lactose).…”

Section: Using Cryptic Plasmids To Create Recombinant Probioticsmentioning

confidence: 99%

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Recombinant Probiotic Preparations: Current State, Development and Application Prospects

Khablenko

Danylenko

Yalovenko

et al. 2023

Innov Biosyst Bioeng

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The article is devoted to the latest achievements in the field of research, development, and implementation of various types of medicinal products based on recombinant probiotics. The benefits of probiotics, their modern use in medicine along with the most frequently used genera and species of probiotic microorganisms were highlighted. The medicinal and therapeutic activities of the studied probiotics were indicated. The review suggests various methods of creating recombinant probiotic microorganisms, including standard genetic engineering methods, as well as systems biology approaches and new methods of using the CRISPR-Cas system. The range of potential therapeutic applications of drugs based on recombinant probiotics was proposed. Special attention was paid to modern research on the creation of new, more effective recombinant probiotics that can be used for various therapeutic purposes. Considering the vast diversity of therapeutic applications of recombinant probiotics and ambiguous functions, their use for the potential treatment of various common human diseases (non-infectious and infectious diseases of the gastrointestinal tract, metabolic disorders, and allergic conditions) was investigated. The prospects for creating different types of vaccines based on recombinant probiotics together with the prospects for their implementation into medicine were considered. The possibilities of using recombinant probiotics in veterinary medicine, particularly for the prevention of domestic animal diseases, were reviewed. The prospects for the implementation of recombinant probiotics as vaccines and diagnostic tools for testing certain diseases as well as modeling the work of the human digestive system were highlighted. The risks of creation, application, including the issues related to the regulatory sphere regarding the use of new recombinant microorganisms, which can potentially enter the environment and cause unforeseen circumstances, were outlined.

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Section: Methods Of Modifying the Genome Of Probioticsmentioning

confidence: 99%

Section: Using Cryptic Plasmids To Create Recombinant Probioticsmentioning

confidence: 99%