Genetic methods for the control of alien species

Махров, А. А.; Karabanov, D. P.; Koduhova, Yu. V.

doi:10.1134/s2075111714030096

Cited by 10 publications

(5 citation statements)

References 39 publications

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“…To date, the TYC approach for any species has been entirely theoretical, and an empirical field test of the approach has yet to be conducted (Wedekind 2012;Makhrov et al 2014). Given the successful production of a YY Brook Trout broodstock that can be used to generate large numbers of M YY fish for stocking (Schill et al 2016) and given the results of the present simulations, field testing is needed to determine whether M YY stocking can be used to eradicate undesirable Brook Trout populations.…”

Section: Alpine Lakes -Good M Yy Survivalmentioning

confidence: 99%

“…In the most comprehensive study to date on population effects of Brook Trout removal in alpine lakes, Hall (1991) concluded that methods designed to reduce recruitment should be evaluated because massive density reductions alone were not effective in suppressing larger populations. Collectively, these and other studies suggest that manual or chemical eradication of undesirable nonnative populations is often impractical (Britton et al 2011) or is feasible only in small waters because in larger populations, a few individuals often survive to repopulate the system (Franssen et al 2014;Makhrov et al 2014). Effective management of large, complex systems requires development of alternative approaches for eliminating nonnative Brook Trout and other undesirable exotics.…”

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confidence: 99%

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Simulated Effects of YY‐Male Stocking and Manual Suppression for Eradicating Nonnative Brook Trout Populations

Schill

Meyer

Hansen

2017

N American J Fish Manag

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Eradication of nonnative Brook Trout Salvelinus fontinalis populations is difficult to achieve with standard techniques, such as electrofishing removal or piscicides; new approaches are needed. A novel concept is to stock “supermale” hatchery fish with wild conspecifics. Supermales (MYY) have two Y‐chromosomes, resulting in offspring that are all males; over time, successful supermale reproduction could eradicate the wild population. We constructed an age‐structured stochastic model to investigate the effects of manually suppressing wild fish and stocking MYY fingerlings on the long‐term viability of hypothetical nonnative Brook Trout populations. In streams, an annual stocking rate of supermales equivalent to 50% of wild age‐0 Brook Trout density combined with an annual selective suppression rate equivalent to 50% of wild Brook Trout density resulted in a time to extirpation of only 2–4 years if supermale fitness was equivalent to wild male fitness. However, time to extirpation in streams was 5–15 years if supermale fitness was 80% lower than wild male fitness. In alpine lakes, higher supermale stocking rates and nonselective gillnetting were required to eradicate Brook Trout populations. If supermales were assumed to be as fit as wild males, however, any supermale stocking rate greater than 49% in alpine lakes or 60% in streams achieved eradication in 10 years or less, regardless of the suppression rate. Because manual suppression and the stocking of MYY fingerlings can readily be conducted at the levels assumed in our simulations, use of such an integrated pest management (IPM) approach could extirpate undesirable Brook Trout populations within reasonably short periods of time. Given the recent successful development of an MYY Brook Trout broodstock capable of producing large numbers of MYY fingerlings and given the positive results of the present simulations for both streams and alpine lakes, field testing of MYY stocking is warranted within an IPM program that includes manual suppression for eradicating undesirable Brook Trout populations. Received October 14, 2016; accepted June 10, 2017 Published online August 22, 2017

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Section: Alpine Lakes -Good M Yy Survivalmentioning

confidence: 99%

mentioning

confidence: 99%

Simulated Effects of YY‐Male Stocking and Manual Suppression for Eradicating Nonnative Brook Trout Populations

Schill

Meyer

Hansen

2017

N American J Fish Manag

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“…Therefore, the first reports of NIS often appear when they have already successfully invaded new ecosystems. Thus, the effectiveness of biological invasion monitoring and developing methods for controlling (and combating with) unwanted species are closely related to their fast and accurate identification [3][4][5][6]. Currently, DNA barcoding based on sequences of the first subunit of mitochondrial cytochrome oxidase c (COI, COX) is regarded sometimes as the ultima ratio for animal identification [7].…”

Section: Introductionmentioning

confidence: 99%

New Sets of Primers for DNA Identification of Non-Indigenous Fish Species in the Volga-Kama Basin (European Russia)

Karabanov

Bekker

Pavlov

et al. 2022

Water

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Adequate species’ identification is critical for the detection and monitoring of biological invasions. In this study, we proposed and assessed the efficiency of newly created primer sets for the genetic identification of non-indigenous species (NIS) of fishes in the Volga basin based on: (a) a “long” fragment of cytochrome c oxidase subunit one of the mitochondrial gene (COI) (0.7 kb), used in “classical” DNA barcoding; (b) a short 3’-fragment (0.3 kb) of COI, suitable for use in high-throughput sequencing systems (i.e., for dietary analysis); (c) fragment of 16S mitochondrial rRNA, including those designed to fill the library of reference sequences for work on the metabarcoding of communities and eDNA studies; (d) a fragment of 18S nuclear rRNA, including two hypervariable regions V1-V2, valuable for animal phylogeny. All four sets of primers demonstrated a high amplification efficiency and high specificity for freshwater fish. Also, we proposed the protocols for the cost-effective isolation of total DNA and purification of the PCR product without the use of commercial kits. We propose an algorithm to carry out extremely cheap studies on the assessment of biological diversity without expensive equipment. We also present original data on the genetic polymorphism of all mass NIS fish species in the Volga-Kama region. The high efficiency of DNA identification based on our primers is shown relative to the traditional monitoring of biological invasions.

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“…This fact makes complicated the management of biological resources in order to reduce the harmful impact of the invaders on indigenous fauna. Thus, the effectiveness of biological invasion monitoring and developing methods for controlling (and combating with) unwanted species is closely related to their fast and accurate identification [7].…”

Section: Introductionmentioning

confidence: 99%

Universal Set of Primers for DNA Identification of Alien Fish Species in Central Russia (Volga-Kama Region)

Karabanov¹,

Ei²,

Dd³

et al. 2021

Preprint

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Reliable species identification is critical for detection and monitoring of biological invasions. In this study, we propose four sets of primers for efficient amplification of several loci, including the mitochondrial cytochrome oxidase-c (COI) subunit I gene which is a basis for DNA barcoding. This set of primers gives a shorter product which can be used in high-throughput sequencing systems for metabarcoding purposes. Another mitochondrial locus encoding the large ribosomal subunit (16S) may be useful to study the population structure and as an additional source of information in the metabarcoding of communities. We propose to use a set of primers for the nuclear locus of the small ribosomal subunit (18S) as a positive control and to verify the results of the barcoding. Our proposed sets of primers demonstrate a high amplification efficiency and a high specificity both for freshwater alien and indigenous fishes. The proposed research design makes it possible to carry out extremely cheap studies on the assessment of biological diversity using genetic analysis without expensive equipment, and with the technique for conducting laboratory work and processing of the results available to any researcher. The paper also presents original data on the genetic polymorphism of all mass alien fish species in the Volga-Kama region. High efficiency of DNA identification based on our primers is shown as compared to traditional monitoring of biological invasions.

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Genetic methods for the control of alien species

Cited by 10 publications

References 39 publications

Simulated Effects of YY‐Male Stocking and Manual Suppression for Eradicating Nonnative Brook Trout Populations

Simulated Effects of YY‐Male Stocking and Manual Suppression for Eradicating Nonnative Brook Trout Populations

New Sets of Primers for DNA Identification of Non-Indigenous Fish Species in the Volga-Kama Basin (European Russia)

Universal Set of Primers for DNA Identification of Alien Fish Species in Central Russia (Volga-Kama Region)

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