Genetic markers for preeclampsia in Peruvian women

Abstract

Background: Preeclampsia is a multiorgan disorder associated with maternal and perinatal morbi-mortality. In Peru, incidence is 10% and accounts for 22% of maternal deaths. Genome and genetic epidemiological studies have found an association between preeclampsia and genetic polymorphisms. Objective: To determine the association of the vascular endothelial growth factor (VEGF) +936 C/T and +405 G/C, interleukine-6 (IL-6) -174 G/C, IL-1β-511 C/T, Apo A-1-75 G/A, Apo B-100 2488 C/T (Xbal) polymorphisms with preeclampsia in pregnant Peruvian women. Methods: Were included preeclamptic and healthy (control) pregnant women. Maternal blood samples were subjected to DNA extraction, and molecular genetic analysis was conducted using the PCR-RFLP technique and following a specific protocol for each gene. Allele and genotypic frequencies in the cases and controls were compared. Results: No association was found between the VEGF+936C/T and VEGF+405 polymorphisms and preeclampsia. The frequencies of the GG genotypes and the G allele of the -174 G/C polymorphism in the IL6 gene in preeclamptic and controls showed significant differences, with higher frequencies in cases. For the -511 C/T polymorphism of the IL-1β gene, no significant differences were found in the frequencies of TT genotypes compared with CT+CC. The genotypes and alleles of the Apo-A1-75 G/A and Apo-B100 Xbal variants showed no significant differences between cases and controls. Conclusion: No association was found between the studied genetic markers and preeclampsia. However, in the -174G/C polymorphism of the IL-6 gene, significant differences were found mainly in the GG genotype and G allele.

Severe Acute Respiratory Syndrome-Coronavirus-2 Antibody Status at the Time of Delivery and the Risk of Preeclampsia

“…The incidence of preeclampsia in our study was 10.42%. These results are consistent with previous studies in Peru, where preeclampsia occurs from 5% to 12% of pregnancies [14,15]. Of all patients with preeclampsia, it is estimated that 25% of them will develop severe features [16].…”

The Role of Dietary Polyphenols in Pregnancy and Pregnancy-Related Disorders

“…Polyphenols are also shown to induce PCDA by inhibiting NO-mediated vasodilatation in pregnant sheep after PRF intake for 14 days [189]. The ductus arteriosus vulnerability to constrictive factors, thus the risk of PCDA, progressively increases with gestational age (e.g., the sensitivity to indomethacin-induced vasoconstriction is approximately 5-10% at 27 weeks, 15-20% at 32 weeks, and almost 100% at 34 weeks [190]. To date, there is no consensus concerning the amount of polyphenol consumption that could cause PCDA.…”

Diagnostic biomolecules and combination therapy for pre-eclampsia

“…A recent study demonstrated that PE diagnostic performance using the sFlt-1/PIGF ratio can achieve 95% sensitivity and 91% specificity which is superior to VEGF, PIGF or sFLt-1 [ 55 ]. Lately, several studies have attempted to use the VEGF genetic polymorphisms for early prediction of PE but there remains a lack of clinical competence [ 56 , 57 ].…”