Genetic maps of RAPD, AFLP and ISSR markers in <i>Ananas bracteatus</i> and <i>A. comosus</i> using the pseudo‐testcross strategy

Carlier, Jorge Dias; Reis, Alessandra Maria Moreira; Duval, Marie-France; D'Eeckenbrugge, Géo Coppens; Leitão, José

doi:10.1046/j.1439-0523.2003.00924.x

Cited by 52 publications

(30 citation statements)

References 22 publications

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“…Plants in the first three classes were considered to be resistant and plants in the last three classes, susceptible (Coelho and Monteiro 2003b). DNA extraction and DNA bulking Plant genomic DNA was extracted according to Carlier et al (2004) with minor modifications. Briefly, approximately 1 g of leaf tissue was ground in a mortar with a pestle in the presence of liquid nitrogen.…”

Section: Plant Materialsmentioning

confidence: 99%

Mapping of a locus for adult plant resistance to downy mildew in broccoli (Brassica oleracea convar. italica)

et al. 2004

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The identification of the gene Pp523, conferring downy mildew resistance to adult plants of broccoli (Brassica oleracea convar. italica), led to the construction of a genetic map that included this resistance locus, 301 amplified fragment length polymorphisms, 55 random amplified polymorphic DNAs, 46 inter-simple sequence repeats, three simple sequence repeats, four other PCR markers and a flower colour locus, all gathered into nine major linkage groups. Nineteen additional molecular markers were clustered into one group of four markers, one group of three markers and six pairs of markers. The map spans over 731.9 cM, corresponding to 89.5% of the 818 cM estimated to be the total genome length. A significant number of the mapped markers, 19.3%, showed distorted segregation. The average distance between mapped adjacent markers is 1.64 cM, which places this map among the densest published to date for this species. Using bulked segregant analysis, we identified a group of molecular markers flanking and closely linked in coupling to the resistance gene and included these in the map. Two markers linked in coupling, OPK17_980 and AT.CTA_133/134, are located at 3.1 cM and 3.6 cM, respectively, at each side from the resistance gene. These markers can be used for marker-assisted selection in breeding programs aiming at the introgression of this gene in susceptible B. oleracea genotypes. The fine mapping of the genomic region surrounding the Pp523 resistance gene is currently being carried out, a basic condition for its isolation via positional cloning.

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Section: Plant Materialsmentioning

confidence: 99%

Mapping of a locus for adult plant resistance to downy mildew in broccoli (Brassica oleracea convar. italica)

et al. 2004

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“…The technique has also been employed to identify varieties in pineapple (Leal et al ARTICLE EY Paz et al 1986) and carry out classical genetic mapping studies (Carlier et al 2004). Kato et al (2004), Paz et al (2005) and Tapia proved its suitability for germplasm evaluation.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of Cuban pineapple germplasm assessed by AFLP Markers

Paz

Gil

Rebolledo

et al. 2012

Crop Breed. Appl. Biotechnol.

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“…Ananas bracteatus é utilizada como planta ornamental, como cerca-viva e na produção de fibras (Carlier et al 2004) e foi registrada em estudo florístico realizado no Parque Municipal de Itajuru, Muriaé, Minas Gerais (Paula et al 2008). Brasiliopuntia.…”

Section: Famílias/espéciesunclassified

Composição florística das espécies vasculares e caráter sucessional da flora arbórea de um fragmento de Floresta Estacional Semidecidual no Sul do Brasil

Costa¹,

Estevan

Bianchini

et al. 2011

Rev. bras. Bot.

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-(Floristic composition of the vascular species and tree flora successional character in a Seasonal Semideciduous Forest remnant in Southern Brazil). The floristic composition of vascular species present in an urban forest remnant in the Ibiporã Forest Park (23°15'71" S and 51°01'83" W, Park entrance), Southern Brazil, was studied from May 2006 to May 2008. The species were divided in life form and tree species were placed in successional categories. A total of 176 native species, 147 genera and 57 families were found. The majority of species were Angiosperms (98%) while 2% were Pteridophyta. The richest families were Fabaceae (19), Bignoniaceae, Malvaceae (nine), Meliaceae and Rubiaceae (eight). The tree species were majority (53%), following by herbaceous (16%), vines (14%), shrubs (13%) and epiphyte species (4%). The successional characterization of the tree species showed a slight predominance of the late secondary species (52%) over species of early succession (45%) IntroduçãoA fragmentação das florestas reduz a área de cobertura total e promove alterações no ambiente florestal, podendo resultar na extinção imediata de algumas espécies e, ao longo do tempo, expõe os organismos sobreviventes aos efeitos do ambiente alterado, o que se tem denominado de efeito de borda (Murcia 1995). A resposta das espécies é variável, porém alterações na sobrevivência, na estrutura e na distribuição das espécies na comunidade contribuem para a perda da diversidade biológica, tanto diretamente pela redução do estoque de genes e de espécies, como indiretamente, em consequência da quebra das interações ecológicas e da estabilidade dos ecossistemas (Futuyma 1996). O conhecimento da flora e da fauna constitui o ponto de partida para o acompanhamento das alterações temporais que podem orientar ações de manejo das unidades de conservação.Na região norte do Estado do Paraná, o processo de ocupação com a lavoura cafeeira a partir da década de 40 gerou um intenso processo de fragmentação da floresta original, restando atualmente vários fragmentos pequenos e isolados (Campos & Costa Filho 2006

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Genetic maps of RAPD, AFLP and ISSR markers in Ananas bracteatus and A. comosus using the pseudo‐testcross strategy

Cited by 52 publications

References 22 publications

Mapping of a locus for adult plant resistance to downy mildew in broccoli (Brassica oleracea convar. italica)

Mapping of a locus for adult plant resistance to downy mildew in broccoli (Brassica oleracea convar. italica)

Genetic diversity of Cuban pineapple germplasm assessed by AFLP Markers

Composição florística das espécies vasculares e caráter sucessional da flora arbórea de um fragmento de Floresta Estacional Semidecidual no Sul do Brasil

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