Genetic maps

Goodfellow, Peter N.; Sefton, Louise; Farr, Carol L.

doi:10.1007/978-94-011-1240-6_1

Cited by 2 publications

(2 citation statements)

References 81 publications

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“…The ends of the BAC were directly sequenced and primers (NFE2BACSP6, F1-TC-CTATTTCACCAGCCAAGAACTG; and NFE2BACSP6, R1-CAAAAGGAGGTTTCTCAGCGATGC) were designed for single-strand conformational polymorphism (SSCP) analysis. A 200-bp band was generated that segregated in the genetic mapping panels (16,20).…”

Section: Isolation Of Cdna Clone For Zebrafish Nfe2mentioning

confidence: 99%

Isolation and characterization of zebrafish NFE2

Pratt

Drejer

Foott

et al. 2002

Physiological Genomics

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Vertebrate hematopoiesis is regulated by distinct cell-specific transcription factors such as GATA-1 and SCL. Mammalian p45-NFE2 was characterized for its ability to bind the hypersensitive sites of the globin locus control region. NFE2 is a member of a cap'n'collar (CNC) and basic zipper (BZIP) superfamily that regulates gene transcription. It has been implicated in diverse processes such as globin gene expression, oxidative stress, and platelet lineage differentiation. Here, we have isolated the zebrafish ortholog of NFE2. The gene is highly homologous, particularly in the DNA-binding domain. Mapping the zebrafish NFE2 to linkage group 23 establishes a region of chromosomal synteny with human chromosome 12, further suggesting evolutionary conservation. During embryogenesis, the zebrafish gene is expressed specifically in erythroid cells and also in the developing ear. NFE2 expression is lacking in zebrafish mutants that have no hematopoietic cells. An analysis of the sauternes mutant, which carries a mutation in the ALAS-2 gene and thus has defective heme synthesis, demonstrates higher levels of NFE2 expression than normal. This further establishes the block to erythroid differentiation in the sauternes mutant. Our studies demonstrate conservation of the vertebrate genetic program for the erythroid lineage.

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Section: Isolation Of Cdna Clone For Zebrafish Nfe2mentioning

confidence: 99%

Isolation and characterization of zebrafish NFE2

Pratt

Drejer

Foott

et al. 2002

Physiological Genomics

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“…Molecular markers have the potential to be used as linked markers for traits that are difficult or time-consuming to select through conventional breeding methods. Restriction fragment length polymorphisms (RFLPs) have been widely used in the development of detailed genetic maps of different crop species (Goodfellow et al 1993). Genetic maps developed using molecular markers spaced throughout the genome offer potential opportunities for marker-assisted selection in breeding programs.…”

Section: Introductionmentioning

confidence: 99%

Detection of quantitative trait loci associated with leaf rust resistance in bread wheat

William¹,

Hoisington²,

Singh³

et al. 1997

Genome

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Leaf rust, caused by Puccinia recondita Rob. ex Desm., is a common disease in wheat. The objective of this study was to develop molecular markers associated with the quantitative trait loci (QTLs) putatively conferring durable leaf rust resistance in Triticum aestivum L. em. Thell. A population of 77 recombinant inbred lines (RILs) developed from 'Parula' (resistant) and 'Siete Cerros' (moderately susceptible) was used. Bulked segregant analysis was done using random amplified polymorphic DNAs (RAPDs) with DNA enriched for low-copy sequences using hydroxyapatite chromatography. Out of 400 decamer primers screened, 3 RAPD markers were identified between the bulk of the most resistant and the bulk of the most susceptible lines. These were cloned and used as probes on the RILs in Southern hybridizations. Two probes revealed two tightly linked loci. Oneway analysis of variance showed that these two loci, and another revealed by the third probe, were linked to QTLs controlling leaf rust resistance based on data taken from 2 years of replicated field trials. Cytogenetic analysis placed the two tightly linked loci on the long arm of chromosome 7B. The third probe detected loci located on the short arms of chromosomes 1B and ID. It is suggested that the QTL detected on 7BL may well be homoeoallelic to Lr34.Key words: bulked segregant analysis, RAPDs, RFLPs, DNA enrichment, leaf rust, QTL.Resume : La rouille brune, causCe par le Puccinia recondita Rob. ex Desm., est une maladie commune chez le blC. L'objectif de ce travail Ctait de mettre au point des marqueurs molCculaires associCs avec les loci h caractkre quantitatif (QTLs) qui confkrent apparemment une rCsistance durable h la rouille brune chez le Triticum aestivum L. em. Thell. Une population de 77 lignCes recombinantes fixCes (RILs) dCrivCes du croisement entre 'Parula' (resistant) et 'Siete Cerros' (modCrCment sensible) a Ct C employCe. Une analyse de sCgrCgants regroupCs (BSA) a Ct C rCalisCe h l'aide de marqueurs RAPD (ADN polymorphe amplifiC au hasard) sur de 1'ADN enrichi en sCquences h simple copie par chromatographie sur colonnes d'hydroxyapatite. Des 400 amorces dCcanuclCotidiques employCes, trois prksentaient un polymorphisme entre 1'ADN des plantes les plus rksistantes et celui des plantes les plus sensibles. Ces fragments polymorphes ont Ct C clonCs et employCs comme sondes pour rCaliser des hybridations Southern sur les RILs. Deux sondes ont rCvClC des loci trks fortement liCs. Une analyse de variance h un critkre de classification a montrC que ces deux loci et un troisikme dCtectC par la troisikme sonde Ctaient liCs h des QTLs contr8lant la rCsistance h la rouille brune telle que mesurCe en essais au champ avec rCplication et sur 2 annCes. Une analyse cytogCnCtique a permis de placer les deux loci fortement liCs sur le bras long du chromosome 7B. La troisikme sonde a dCtectC des loci situCs sur les bras courts des chromosome 1B et ID. I1 est suggCrC que le QTL situC sur le chromosome 7BL pourrait bien etre homCoallClique au locus Lr34.

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Genetic maps

Cited by 2 publications

References 81 publications

Isolation and characterization of zebrafish NFE2

Isolation and characterization of zebrafish NFE2

Detection of quantitative trait loci associated with leaf rust resistance in bread wheat

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