Genetic mapping of the loquat canker resistance gene pse-c in loquat ( Eriobotrya japonica )

Fukuda, Shinji; Ishimoto, Keiichiro; Terakami, Shingo; Yamamoto, Takatsugu; Hiehata, Naofumi

doi:10.1016/j.scienta.2015.12.036

Cited by 5 publications

(4 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was further suggested that there is more than one gene involved in the disease tolerance in loquat fruit that cannot be determined by the phenotype or genotype of the Psc-c locus gene. Based on disease symptoms, Pseudomonas has been classified into three potential groups, A, B, and C, from which only group C pathogens cause brown spots on the skin of leaves and fruit [ 174 ]. However, future research on the canker control strategies in loquat on its transmission, mode of action, and control strategies in loquat fruit is warranted.…”

Section: Diseasesmentioning

confidence: 99%

Postharvest Biology and Technology of Loquat (Eriobotrya japonica Lindl.)

Shah

Khan

Singh

et al. 2023

Foods

View full text Add to dashboard Cite

Loquat (Eriobotrya japonica Lindl.) fruit is a rich source of carotenoids, flavonoids, phenolics, sugars, and organic acids. Although it is classified as a non-climacteric fruit, susceptibility to mechanical and physical bruising causes its rapid deterioration by moisture loss and postharvest decay caused by pathogens. Anthracnose, canker, and purple spot are the most prevalent postharvest diseases of loquat fruit. Cold storage has been used for quality management of loquat fruit, but the susceptibility of some cultivars to chilling injury (CI) consequently leads to browning and other disorders. Various techniques, including cold storage, controlled atmosphere storage, hypobaric storage, modified atmosphere packaging, low-temperature conditioning, heat treatment, edible coatings, and postharvest chemical application, have been tested to extend shelf life, mitigate chilling injury, and quality preservation. This review comprehensively focuses on the recent advances in the postharvest physiology and technology of loquat fruit, such as harvest maturity, fruit ripening physiology, postharvest storage techniques, and physiological disorders and diseases.

show abstract

Section: Diseasesmentioning

confidence: 99%

Postharvest Biology and Technology of Loquat (Eriobotrya japonica Lindl.)

Shah

Khan

Singh

et al. 2023

Foods

View full text Add to dashboard Cite

show abstract

“…Many classic breeding approaches to improve this crop are underway [8, 9]. Moreover, considerable research has focused on the genetics and molecular mechanisms underlying specific biological traits/phenomena in loquat for exploiting molecular markers for assisted selection [10, 11] or identifying important genes that may be directly or indirectly used in future breeding endeavors [2, 12–15]. Overall, gene expression analysis is one of the most important approaches for identifying candidate genes related to specific biological characteristics.…”

Section: Introductionmentioning

confidence: 99%

Selection of the optimal reference genes for expression analyses in different materials of Eriobotrya japonica

Yuan

Zhang

et al. 2019

Plant Methods

View full text Add to dashboard Cite

BackgroundLoquat (Eriobotrya japonica) is a subtropical tree bearing fruit that ripens during late spring and early summer, which is the off-season for fruit production. The specific flowering habit of loquat, which starts in fall and ends in winter, has attracted an increasing number of researchers who believe that it may represent an ideal model for studying flowering shift adaptations to climate change in Rosaceae. These studies require an understanding of gene expression patterns within the fruit and other tissues of this plant. Although ACTINs (ACTs) have previously been used as reference genes (RGs) for gene expression studies in loquats, a comprehensive analysis of whether these RGs are optimal for normalizing RT-qPCR data has not been performed.ResultsIn this study, 11 candidate RGs (RIBOSOMAL-LIKE PROTEIN4 (RPL4), RIBOSOMAL-LIKE PROTEIN18 (RPL18), Histone H3.3 (HIS3), Alpha-tubulin-3 (TUA3), S-Adenosyl Methionine Decarboxylase (SAMDC), TIP41-like Family Protein (TIP41), (UDP)-glucose Pyrophosphorylase (UGPase), 18S ribosomal RNA (18S), Glyceraldehyde-3-phosphate Dehydrogenase (GAPDH), Plasma Intrinsic Protein 2 (PIP2) and ACTIN(ACT)) were assessed to determine their expression stability in 23 samples from different tissues or organs of loquat. Integrated expression stability evaluations using five computational statistical methods (GeNorm, NormFinder, ΔCt, BestKeeper, and RefFinder) suggested that a RG set, including RPL4, RPL18, HIS3 and TUA3, was the most stable one across all of the tested loquat samples. The expression pattern of EjCDKB1;2 in the tested loquat tissues normalized to the selected RG set demonstrated its reliability.ConclusionsThis study reveals the reliable RGs for accurate normalization of gene expression in loquat. In addition, our findings demonstrate an efficient system for identifying the most effective RGs for different organs, which may be applied to related rosaceous crops.Electronic supplementary materialThe online version of this article (10.1186/s13007-019-0391-2) contains supplementary material, which is available to authorized users.

show abstract

“…eriobotryae is a pathogenic bacterium that causes stem canker in loquat trees. The pathogen attacks all plant parts, causing weak growth and reduction of commercial value of fruits [ 1 , 2 ]. Damage from this disease has been documented in Argentina, Australia, China, Japan, New Zealand, and the US [ 3 , 4 , 5 , 6 , 7 , 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

“…The wild species bronze loquat ( Eriobotrya deflexa ) and was mapped [ 12 ] to reveal the presence of Pse-a , with the constructed linkage group spanned by 69.4 cM and had an average marker density of 2.6 cM [ 12 ]. Resistance to the group C bacteria was controlled by a recessive gene Pse-c [ 20 ], and mapped [ 2 ]. Hiehata et al [ 21 ] screened 52 loquat cultivars for resistance to groups A, B, and C. There were 25 resistant cultivars, whereas 22 were susceptible to group A.…”

Section: Introductionmentioning

confidence: 99%

Association and Expression of Virulence from Plasmids of the Group B Strain in Pseudomonas syringae pv. eriobotryae

Khanh

Xuan

2018

Pathogens

View full text Add to dashboard Cite

Pseudomonas syringae pv. eriobotryae causes serious stem canker in loquat (Eriobotrya japonica) trees. This study was conducted to determine whether plasmids are involved with its virulence. The strain NAE89, which belonged to the B group, harbored two plasmids at approximately 6.2 and 50 Mdal that caused stem canker and halo leaf spots on loquat plants. Following digestion with BamHI and ligation into the BamHI cloning site of the broad range host cosmid pLAFR3, four DNA fragments at 3.8, 6.6, 12.3, and 22.8 kb were generated. Although the plasmid-encoded virulence gene psvA was undigested with the BamHI, the halo leaf spot gene may be adjacent to the psvA gene was digested. A pLAFR3 cosmid clone was introduced into the non-pathogenic PE0 and NAE89-1 strains by triparental matings and the pathogenicity was recovered. As a result, the pLAFR3 cosmid clone was introduced into the largest size DNA fragment of 22.8 kb and determined to be the causal agent of canker on the stem of the loquat. This study revealed that the psvA gene, previously found in the 50 Mdal plasmid, was also observed in the 22.8 kb DNA fragment.

show abstract

Genetic mapping of the loquat canker resistance gene pse-c in loquat ( Eriobotrya japonica )

Cited by 5 publications

References 27 publications

Postharvest Biology and Technology of Loquat (Eriobotrya japonica Lindl.)

Postharvest Biology and Technology of Loquat (Eriobotrya japonica Lindl.)

Selection of the optimal reference genes for expression analyses in different materials of Eriobotrya japonica

Association and Expression of Virulence from Plasmids of the Group B Strain in Pseudomonas syringae pv. eriobotryae

Contact Info

Product

Resources

About