Genetic Mapping of the HLA1 Locus Causing Hybrid Lethality in Nicotiana Interspecific Hybrids

Tezuka, Takahiro; Kitamura, Naoto; Imagawa, Sae; Hasegawa, Akira; Shiragaki, Kumpei; He, Hai; Yanase, Masanori; Ogata, Yasuhiro; Morikawa, Tomomi; Yokoi, Shuji

doi:10.3390/plants10102062

Cited by 7 publications

(9 citation statements)

References 63 publications

(83 reference statements)

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“…Conspecific pollen precedence and gametic incompatibility are examples of post-mating prezygotic isolating barriers ( Rieseberg and Willis, 2007 ). In turn, postzygotic isolation barriers include enlarged ovary (immature fruit) abscission ( Gupta et al, 1996 ; He et al, 2019 ), seed abortion or hybrid seed lethality ( Coughlan et al, 2020 ; Dziasek et al, 2021 ), hybrid weakness ( Shiragaki et al, 2019 , 2020 ), hybrid lethality ( Kawaguchi et al, 2021 ; Si et al, 2021 ; Tezuka et al, 2021 ; Mino et al, 2022 ), and hybrid sterility ( Li et al, 2020 ) in seedlings of the F 1 generation, as well as hybrid breakdown recognized in generations after the F 1 ( Matsubara et al, 2007 ; Zhang et al, 2021 ). These isolating barriers may be observed independently or combined, even in a single cross-combination.…”

Section: Introductionmentioning

confidence: 99%

Parental Genome Imbalance Causes Hybrid Seed Lethality as Well as Ovary Abscission in Interspecific and Interploidy Crosses in Nicotiana

Sadahisa

Yokoi

et al. 2022

Front. Plant Sci.

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Enhanced ovary abscission after pollination and hybrid seed lethality result in post-zygotic reproductive isolation in plant interspecific crosses. However, the connection between these barriers remains unclear. Here, we report that an imbalance in parental genomes or endosperm balance number (EBN) causes hybrid seed lethality and ovary abscission in both interspecific and intraspecific-interploidy crosses in the genus Nicotiana. Auxin treatment suppressed ovary abscission, but not hybrid seed lethality, in an interspecific cross between Nicotiana suaveolens and N. tabacum, suggesting that ovary abscission-related genes are located downstream of those involved in hybrid seed lethality. We performed interploidy crosses among N. suaveolens tetraploids, octoploids, and neopolyploids and revealed hybrid seed lethality and ovary abscission in interploid crosses. Furthermore, a higher maternal EBN than paternal EBN caused these barriers, as previously observed in N. suaveolens × N. tabacum crosses. Altogether, these results suggest that maternal excess of EBN causes hybrid seed lethality, which in turn leads to ovary abscission through the same mechanism in both interspecific and interploidy crosses.

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Section: Introductionmentioning

confidence: 99%

Parental Genome Imbalance Causes Hybrid Seed Lethality as Well as Ovary Abscission in Interspecific and Interploidy Crosses in Nicotiana

Sadahisa

Yokoi

et al. 2022

Front. Plant Sci.

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“…In the Nicotiana section Suaveolentes, a high degree of chromosome pairing in meiosis has been reported in several interspecific hybrids [10,22,[25][26][27][28][29][30][31][32]. Reciprocal hybrids between N. benthamiana JT and N. excelsior JT also showed a high degree of chromosome pairing, suggesting high homology of the genomes.…”

Section: Discussionmentioning

confidence: 92%

“…However, the transfer of desirable genes of Suaveolentes species into N. tabacum has been disrupted by reproductive isolation [19]. Hybrid lethality, a type of postzygotic reproductive isolation, is commonly observed in crosses between N. tabacum and most Suaveolentes species, including N. excelsior [14,[19][20][21][22]. Hybrid lethality is a phenomenon that causes the death of F 1 hybrid seedlings.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Crossability between Nicotiana benthamiana and Nicotiana excelsior

et al. 2021

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Wild tobacco species in the Nicotiana section Suaveolentes are promising genetic resources to introduce their disease resistance to cultivated tobacco, Nicotiana tabacum. However, hybrid lethality is observed in hybrid seedlings from crosses between most Suaveolentes species and N. tabacum. In particular, N. benthamiana belonging to the section Suaveolentes produces only viable hybrids after crossing with N. tabacum. In the present study, crossability between N. benthamiana and N. excelsior (section Suaveolentes) was investigated to test the possible usefulness of N. benthamiana as the bridge parent to transfer desirable genes of N. excelsior to N. tabacum via bridge crossing. After reciprocal crosses using three accessions of N. benthamiana and N. excelsior each, several crossing barriers such as cross-incompatibility, seed abortion, and male and female hybrid sterility were observed. Although reciprocal hybrids between N. benthamiana and N. excelsior showed a high degree of chromosome pairing in meiosis, univalents and multivalents, as well as chromosome bridges and lagging chromosomes, were observed. These meiotic abnormalities were thought to cause hybrid sterility. The possible usefulness of reciprocal hybrids between N. benthamiana and N. excelsior is discussed.

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“…Recently, NtHL1 was isolated by transposon tagging; this gene codes a coiled-coil nucleotide-binding site-leucine-rich repeat (CC-NBS-LRR) protein, which is probably involved in disease resistance ( Ma et al., 2020 ). Its counterpart, HLA1 , has been mapped by the linkage analysis ( Tezuka et al., 2021 ). Identification of the HLA1 will enable a deeper understanding of the interaction between NtHL1 and HLA1 .…”

Section: Hybrid Seedling Lethalitymentioning

confidence: 99%

Understanding and overcoming hybrid lethality in seed and seedling stages as barriers to hybridization and gene flow

Shiragaki

Tezuka³

2023

Front. Plant Sci.

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Hybrid lethality is a type of reproductive isolation barrier observed in two developmental stages, hybrid embryos (hybrid seeds) and hybrid seedlings. Hybrid lethality has been reported in many plant species and limits distant hybridization breeding including interspecific and intergeneric hybridization, which increases genetic diversity and contributes to produce new germplasm for agricultural purposes. Recent studies have provided molecular and genetic evidence suggesting that underlying causes of hybrid lethality involve epistatic interaction of one or more loci, as hypothesized by the Bateson–Dobzhansky–Muller model, and effective ploidy or endosperm balance number. In this review, we focus on the similarities and differences between hybrid seed lethality and hybrid seedling lethality, as well as methods of recovering seed/seedling activity to circumvent hybrid lethality. Current knowledge summarized in our article will provides new insights into the mechanisms of hybrid lethality and effective methods for circumventing hybrid lethality.

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Genetic Mapping of the HLA1 Locus Causing Hybrid Lethality in Nicotiana Interspecific Hybrids

Cited by 7 publications

References 63 publications

Parental Genome Imbalance Causes Hybrid Seed Lethality as Well as Ovary Abscission in Interspecific and Interploidy Crosses in Nicotiana

Parental Genome Imbalance Causes Hybrid Seed Lethality as Well as Ovary Abscission in Interspecific and Interploidy Crosses in Nicotiana

Evaluation of Crossability between Nicotiana benthamiana and Nicotiana excelsior

Understanding and overcoming hybrid lethality in seed and seedling stages as barriers to hybridization and gene flow

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