Genetic mapping of the black tiger shrimp Penaeus monodon with amplified fragment length polymorphism

Wilson, Kate; Li, Yutao; Whan, Vicki; Lehnert, S. A.; Byrne, Keren; Moore, S. S.; Pongsomboon, Siriporn; Tassanakajon, Anchalee; Rosenberg, George H.; Ballment, E.; Fayazi, Zahra; Swan, Jennifer; Kenway, Matthew; Benzie, John A. H.

doi:10.1016/s0044-8486(01)00842-0

Cited by 195 publications

(177 citation statements)

References 18 publications

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“…Only two females from the eight evaluated individuals showed all their bands to be present in the four evaluated families. The number of polymorphic loci detected (7.1 bands per primer combination) is lower than the 14.2 and 10.0 loci reported in P. japonicus and P. monodon (Moore et al, 1999;Wilson et al, 2002). It is not clear if this difference is due to intrinsic characteristics of the P. vannamei genome or a high level of inbreeding in our mapping population.…”

Section: Discussioncontrasting

confidence: 76%

“…The implemented protocols allowed for high data throughput at low cost. The use of silver staining for visualization of DNA fragments gave a high detection ratio (54 bands per gel) which compares favorably with other projects using radioactive and fluorescent automated detection (50 to 75 bands per primer pair) (Wilson et al, 2002). Silver staining is a technique easily implemented and that does not require the use of specialized equipment such as automated sequencers.…”

Section: Discussionmentioning

confidence: 70%

“…Microsatellite genotyping is very useful for parentage assignment in shrimp (Moore et al, 1999;Wilson et al, 2002). With dominant markers, such as AFLPs, each DNA band from a parent should be present in either 100% or 50% of the progeny depending on the parent being homozygous or heterozygous.…”

Section: Discussionmentioning

confidence: 99%

“…Commercial breeding programs for P. vannamei have been initiated in Colombia, USA and Ecuador, whereas Penaeus (Marsopenaeus) japonicus is being selected for increased growth in Australia. Despite its commercial importance, no successful commercial breeding programs have been reported for P. monodon due to problems with closure of the life cycle (Wilson et al, 2002). All the breeding programs implemented on shrimp species might benefit from the use of molecular markers and linkage maps.…”

Section: Introductionmentioning

confidence: 99%

“…All the breeding programs implemented on shrimp species might benefit from the use of molecular markers and linkage maps. In fact two maps have been reported on shrimps (Moore et al, 1999;Wilson et al, 2002;.…”

Section: Introductionmentioning

confidence: 99%

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A sex-specific linkage map of the white shrimp Penaeus (Litopenaeus) vannamei based on AFLP markers

et al. 2004

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We report the construction of sex-specific linkage maps for the white shrimp Penaeus (Litopenaeus) vannamei (Penaeidae; Crustacea). Linkage information was generated using amplified fragment length polymorphism (AFLP) markers in a mapping panel consisting of 42 individuals derived from a commercial cross. We used 103 primer combinations that produced 741 segregating bands. From them, 477 segregated in a 1:1 model, 181 in a 3:1 model, 62 fitted both models and 21 fitted neither model. A total of 394 loci with a 1:1 segregation ratio were mapped to unique positions in the male and female maps using a pseudotestcross strategy. A total of 51 and 47 linkage groups were detected for the male and female maps respectively, in comparison to 44 haploid groups expected from the karyotype. The female map covered 2771 Kosambi units (cM) and was 24% longer than the male map (2116 cM long). The distribution of the markers showed that both maps had low saturation and clustering at short linkage distances. Markers with a distorted segregation were observed as previously reported in other shrimp species. The estimated genomic length indicates that the P. vannamei genome has higher recombination rates than closely related species. We demonstrate the feasibility of implementing molecular techniques

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Section: Discussioncontrasting

confidence: 76%

Section: Discussionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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