Genetic mapping of resistance factors to<i>Phytophthora palmivora</i>in cocoa

Flament, Marie-Henriette; Kébé, Ismael S.; Clément, Didier; Pieretti, Isabelle; Risterucci, A. M.; N'Goran, Jeanne A.K.; Cilas, Christian; Despréaux, Denis; Lanaud, Claire

doi:10.1139/g00-099

Cited by 36 publications

(29 citation statements)

References 19 publications

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“…Indeed, UPA 402 is an Upper-Amazon Forastero clone obtained from a sibmating involving two Forastero genotypes collected in Peru and may be highly homozygous for this linkage group. A similar situation, where a small number of markers were also assigned to LG8, was observed in other maps established by Clement et al (2003) and Flament et al (2001).…”

Section: Discussionsupporting

confidence: 80%

“…These maps were based on codominant markers such as restriction fragment length polymorphisms (RFLPs), isoenzymes and a small number of simple sequence repeats (SSRs) and, in some of the maps, associated with dominant amplified fragment length polymorphisms (AFLP) and random amplified polymorphic DNA (RAPD) markers. They have been used to locate quantitative trait loci (QTLs) affecting traits of interest, such as disease resistance and yield factors (Crouzillat et al 2000;Flament et al 2001;Clement et al 2003;Queiroz et al 2003;Risterucci et al 2003).…”

Section: Introductionmentioning

confidence: 99%

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A new cacao linkage map based on codominant markers: development and integration of 201 new microsatellite markers

et al. 2004

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A linkage map of cacao based on codominant markers has been constructed by integrating 201 new simple sequence repeats (SSR) developed in this study with a number of isoenzymes, restriction fragment length polymorphisms (RFLP), microsatellite markers and resistance and defence gene analogs (Rgenes-RFLP) previously mapped in cacao. A genomic library enriched for (GA)(n) and (CA)(n) was constructed, and 201 new microsatellite loci were mapped on 135 individuals from the same mapping population used to establish the first reference maps. This progeny resulted from a cross between two heterozygous cacao clones: an Upper-Amazon Forastero (UPA 402) and a Trinitario (UF 676). The new map contains 465 markers (268 SSRs, 176 RFLPs, five isoenzymes and 16 Rgenes-RFLP) arranged in ten linkage groups corresponding to the haploid chromosome number of cacao. Its length is 782.8 cM, with an average interval distance between markers of 1.7 cM. The new microsatellite markers were distributed throughout all linkage groups of the map, but their distribution was not random. The length of the map established with only SSRs was 769.6 cM, representing 94.8% of the total map. The current level of genome coverage is approximately one microsatellite every 3 cM. This new reference map provides a set of useful markers that is transferable across different mapping populations and will allow the identification and comparison of the most important regions involved in the variation of the traits of interest and the development of marker-assisted selection strategies.

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Section: Discussionsupporting

confidence: 80%

Section: Introductionmentioning

confidence: 99%

A new cacao linkage map based on codominant markers: development and integration of 201 new microsatellite markers

et al. 2004

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“…Several regions of the genome involved in yield components and Phytophthora spp. resistance were identified (Crouzillat et al 2000;Flament et al 2001;Lanaud et al 2002;ClØment et al 2003aClØment et al , 2003bRisterucci et al 2003) and confirmed the polygenic nature of this quantitative resistance in cocoa. A region of chromosome 6 appears particularly interesting and gave QTL for yield components and resistance to Phytophthora.…”

Section: Introductionmentioning

confidence: 82%

Creation of BAC genomic resources for cocoa (Theobroma cacao L.) for physical mapping of RGA containing BAC clones

et al. 2004

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We have constructed and validated the first cocoa ( Theobroma cacao L.) BAC library, with the aim of developing molecular resources to study the structure and evolution of the genome of this perennial crop. This library contains 36,864 clones with an average insert size of 120 kb, representing approximately ten haploid genome equivalents. It was constructed from the genotype Scavina-6 (Sca-6), a Forastero clone highly resistant to cocoa pathogens and a parent of existing mapping populations. Validation of the BAC library was carried out with a set of 13 genetically-anchored single copy and one duplicated markers. An average of nine BAC clones per probe was identified, giving an initial experimental estimation of the genome coverage represented in the library. Screening of the library with a set of resistance gene analogues (RGAs), previously mapped in cocoa and co-localizing with QTL for resistance to Phytophthora traits, confirmed at the physical level the tight clustering of RGAs in the cocoa genome and provided the first insights into the relationships between genetic and physical distances in the cocoa genome. This library represents an available BAC resource for structural genomic studies or map-based cloning of genes corresponding to important QTLs for agronomic traits such as resistance genes to major cocoa pathogens like Phytophthora spp ( palmivora and megakarya), Crinipellis perniciosa and Moniliophthora roreri.

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“…The genetic reference map of Theobroma cacao L. has been developed using the progeny UPA402 × UF676 (Lanaud et al 1995;Pugh et al 2004). And, QTLs for resistance to Phytophthora also, for agronomic and quality traits were identified (Lanaud et al 1999;Flament et al 2001;Risterucci et al 2003;Clément et al 2003a,b).…”

Section: Africamentioning

confidence: 99%