Genetic Mapping of QTLs for Tissue-Culture Response in Plants

Bolibok, Hanna; Rakoczy-Trojanowska, Monika

doi:10.1007/s10681-005-9055-6

Cited by 32 publications

(32 citation statements)

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“…QTLs for tissue culture response have been mapped for numerous plant species, both dicots and monocots, including major cereals such as rice, wheat, maize and barley [9]. The one existing paper on the molecular analysis of the in vitro response of rye describes only very preliminary results concerning anther culture [26].…”

Section: Discussionmentioning

confidence: 99%

“…With the recent advances in DNA-based molecular marker systems and the development of quantitative trait loci (QTL) mapping technology, it has become possible to resolve complex traits into several single Mendelian components, and to characterize their map position, gene action and phenotypic effect. [9]. There are several examples of identifying QTLs for the in vitro response in cereals, e.g.…”

Section: Introductionmentioning

confidence: 99%

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The identification of QTLs associated with the in vitro response of rye (Secale cereale L.)

Bolibok

Gruszczynska

Hromada-Judycka

et al. 2007

Cellular and Molecular Biology Letters

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This study was conducted in order to identify quantitative trait loci (QTLs) for the in vitro culture response of winter rye (Secale cereale L.) immature embryos and immature inflorescences. A genetic linkage map comprising 67 SSRs, 9 ISSRs, 13 SAMPLs, 7 RAPDs, 2 SCARs and one EST marker was created based on the analyses of 102 recombinant inbred lines from the cross between lines L318 (which has a good response in tissue cultures) and L9 (which is unable to regenerate plants from somatic tissues and anthers). The map spans 979.2 cM, and the average distance between markers is 9.9 cM. Two characteristics were evaluated: callus induction (CI) and somatic embryogenesis ability (SE). They were expressed as the percentage of immature embryos/inflorescences producing callus (designated ECI/ICI) and the percentage of explants producing somatic embryos (ESE/ISE). All the analysed traits showed continuous variation in the mapping population but a non-normal frequency distribution. We identified nine putative QTLs controlling the tissue culture response of rye, explaining up to 41.6% of the total phenotypic variation: two QTLs for ECI — eci-1, eci-2; 4 for ESE — ece-1, ese-2, ese-3, ese-4; 2 for ICI — ici-1, ici2; and 1 for ISE — ise-1. They were detected on chromosomes 1R, 4R, 5R, 6R and 7R.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The identification of QTLs associated with the in vitro response of rye (Secale cereale L.)

Bolibok

Gruszczynska

Hromada-Judycka

et al. 2007

Cellular and Molecular Biology Letters

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“…Although mechanisms of regeneration in dicots have been studied extensively by means of Mendelian genetic studies (Henry et al, 1994), few reports describe QTLs studies of tissue culture capability performed in dicotylodenous plants, such as in tomato, Arabidopsis, sunflower, poplar and broccoli (Bolibok and Rakoczy-Trojanowska, 2006). In this study, callus induction frequency (CIF) and somatic embryos initiation frequency (SEIF) were investigated in terms of the percentages of explants producing calluses and the percentage of explants producing somatic embryos, respectively.…”

Section: Discussionmentioning

confidence: 99%

“…However, a highly efficient regeneration system is a necessary prerequisite for successful utilization of such methods (Bolibok and Rakoczy-Trojanowska, 2006;Bolibok et al, 2007). So far, a number of regeneration systems and transformation systems have been established for many plant species.…”

Section: Introductionmentioning

confidence: 99%

Mapping QTLs for Tissue Culture Response in Soybean (Glycine max (L.) Merr.)

Yang

Zhao

et al. 2011

Molecules and Cells

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Quantitative trait loci (QTLs) that control the tissue culture response in soybean were detected by using 184 recombinant inbred lines (RILs) derived from two varieties: Kefeng No.1 and Nannong 1138-2. The molecular map consisting of 834 molecular markers using this population covered space 2307.83 cM of the genome throughout 24 linkage groups. The performance of tissue culture in soybean was evaluated by two indices: callus induction frequency (CIF) and somatic embryos initiation frequency (SEIF). They were expressed as the number of explants producing callus/ the number of total explants and the number of explants producing somatic embryos/ the number of total explants, respectively. The RIL lines showed continuous segregation for both indices. With the composite interval mapping (CIM) described in Windows QTL Cartographer Version 2.5, three quantitative trait loci (QTLs) were identified for the frequency of callus induction, on chromosomes B2 and D2, accounting for phenotypic variation from 5.84% to 16.60%; four QTLs on chromosome G were detected for the frequency of somatic embryos initiation and explained the phenotypic variation from 7.79% to 14.16%. The information of new QTLs identified in the present study will contribute to genetic improvement of regeneration traits with marker-assisted selection (MAS) in soybean.

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“…To date, no QTL studies on androgenetic capacity in perennial ryegrass have been published. In the cereals, a limited number of reports exist, typically finding between 1 and 8 QTL for each component trait, explaining 3%-65% of the variation [72]. For example, two QTL, explaining 53% of the variation in green plant regeneration, were recently found in wheat [73].…”

Section: Doubled Haploids In Perennial Ryegrassmentioning

confidence: 99%

Haploid and Doubled Haploid Techniques in Perennial Ryegrass (Lolium perenne L.) to Advance Research and Breeding

2016

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Abstract:The importance of haploid and doubled haploid (DH) techniques for basic and applied research, as well as to improve the speed of genetic gain when applied in breeding programs, cannot be overstated. They have become routine tools in several major crop species, such as maize (Zea mays L.), wheat (Triticum aestivum L.), and barley (Hordeum vulgare L.). DH techniques in perennial ryegrass (Lolium perenne L.), an important forage species, have advanced to a sufficiently successful and promising stage to merit an exploration of what their further developments may bring. The exploitation of both in vitro and in vivo haploid and DH methods to (1) purge deleterious alleles from germplasm intended for breeding; (2) develop mapping populations for genetic and genomic studies; (3) simplify haplotype mapping; (4) fix transgenes and mutations for functional gene validation and molecular breeding; and (5) hybrid cultivar development are discussed. Even with the comparatively modest budgets of those active in forage crop improvement, haploid and DH techniques can be developed into powerful tools to achieve the acceleration of the speed of genetic gain needed to meet future agricultural demands.

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Genetic Mapping of QTLs for Tissue-Culture Response in Plants

Cited by 32 publications

References 45 publications

The identification of QTLs associated with the in vitro response of rye (Secale cereale L.)

The identification of QTLs associated with the in vitro response of rye (Secale cereale L.)

Mapping QTLs for Tissue Culture Response in Soybean (Glycine max (L.) Merr.)

Haploid and Doubled Haploid Techniques in Perennial Ryegrass (Lolium perenne L.) to Advance Research and Breeding

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