Genetic mapping of plant disease resistance gene homologues using a minimal <i>Brassica napus</i> L. population

Joyeux, Alexandre; Fortin, Marc; Mayerhofer, Reinhold; Good, Allen G.

doi:10.1139/g99-004

Cited by 22 publications

(8 citation statements)

References 20 publications

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“…The RGA primers (Table 1) were designed from the conserved sequences of LRR, NBS, and PtoKin motifs from cloned resistance genes (Bent et al, 1994;Kanazin et al, 1996;Leister et al, 1996;Yu et al, 1996;Chen et al, 1998;Joyeux et al, 1999;Seah et al, 1998;Rajesh et al, 2002).…”

Section: Rga Analysismentioning

confidence: 99%

Resistance gene analogs associated with Fusarium head blight resistance in wheat

Guo

Bai

et al. 2006

Euphytica

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Fusarium head blight (FHB) is one of the most destructive diseases in wheat. Identification of resistance gene analogs (RGAs) may provide candidate genes for cloning of FHB resistance genes and molecular markers for marker-assisted improvement of wheat FHB resistance. To identify potential RGAs associated with FHB resistance in wheat, 18 primer pairs of RGAs were screened between two parents (Ning7840 and Clark) and seven informative RGA primer combinations were analyzed in their recombinant inbred lines (RILs). Five PCR products amplified from three primer combinations showed significant association with FHB resistance, and their sequences are similar to the gene families of RGAs. Three of them were putatively assigned to chromosome 1AL and explained 12.73%, 5.57% and 5.9% of the phenotypic variation for FHB response in the F 7 population, and 10.37%, 3.37% and 4.53% in F 10 population, respectively; suggesting that these RGAs may play a role in enhancing FHB resistance in wheat. Analysis of nucleotide sequence motifs demonstrated that all the RGA markers contain a heat shock factor that initiates the production of heat shock proteins. A sequence tagged site (STS) marker (FHBSTS1A-160) was successfully converted from RGA18-356, and validated in fourteen other cultivars. Significant interaction between the quantitative trait locus (QTL) on 1AL and the QTL on 3BS was detected. The marker FHBSTS1A-160 in combination with markers linked to the major QTL on 3BS could be used in marker-assisted selection (MAS) for enhanced FHB resistance in wheat.

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Section: Rga Analysismentioning

confidence: 99%

Resistance gene analogs associated with Fusarium head blight resistance in wheat

Guo

Bai

et al. 2006

Euphytica

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“…Furthermore, using the few conserved motifs to construct degenerate PCR primers, it has been possible to clone R-gene analogs (RGAs) from many plant species, e.g. rice and barley (Leister et al, 1996), potato (Lawrence et al, 1995), tomato (Pan et al, 2000), pepper (Parker et al, 1997), soybean (Joyeuk et al, 1999;Witham et al, 1994), common bean (Leister et al, 1998;Pitrat & Lecoq, 1980), Arabidopsis (Song et al, 1995), lettuce (Saitou & Nei, 1987), Brassica (Li et al, 1999), pea (Takada, 1979), and melon (Brotman et al, 1998, Garcia-Mas et al, 2001.…”

Section: Discussionmentioning

confidence: 99%

“…Based on sequence similarity between R genes, a method using degenerate primers to target the conserved motifs has been successfully employed to isolate resistance gene analogs (RGAs) from potato (Lawrence et al, 1995), soybean (Joyeuk et al, 1999;Witham et al, 1994), rice (Leister et al, 1996), Arabidopsis spp. (Aatrs et al, 1998), lettuce (Saitou & Nei, 1987), common bean (Geffroy et al, 1999;Leister et al, 1998;Pitrat & Lecoq, 1980), melon (Brotman et al, 1998), and other monocotyledonous and dicotyledonous.…”

Section: Introductionmentioning

confidence: 99%

Application of Molecular Biology for Identification of Virus Resistance Gene in Melon

Daryono¹

2016

IJBiotech

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Source of resistance to an Indonesia isolate of Cucumber mosaic virus (CMV-B2) in melon cultivar Yamatouri has been reported. Moreover, Creb-2, a locus that confers resistance to CMV-B2 in Yamatouri has been determined as a single dominant gene. To elucidate the resistance mechanism conferred by Creb-2 in more detail, it is necessary to clone the Creb-2 gene and determine its molecular structure. One approach is by amplifi cation and cloning of melon resistance gene analogs (MRGAs) based on degenerated PCR primers designed from conserved amino acids in the NBS-LRR motifs (P-loop, Kinase-2, and the GLPL) and Toll/ Interleukin-1 receptor-like region (TIR). This study was aimed to identify and characterize the resistance gene analogs from Cucumis melo L. cv. Yamatouri by employing polymerase chain reactions (PCR) as a molecular biology tools with degenerate primers based on conserved motifs of cloned R genes. The application of molecular biology such as DNA isolation, degenerate primers and PCR condition, cloning, sequencing, linkage analysis and mapping of resistance gene analogs to Creb-2 gene in melon will be widely discussed in this paper.

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“…The presence of conserved domains in resistance genes gave an opportunity to clone numerous additional resistance genes from diverse species by polymerase chain reaction (PCR) with degenerate oligo-nucleotide primers to the conserved motifs (Joyeux et al, 1999;Deng et al, 2000;Donald et al, 2002;Lacock et al, 2003;Totad et al, 2005). Aarts et al (1998) reported that 1 to 2% of the total coding capacity of the Arabidopsis genome is contributed by the NBS-LRR sequences, and genetic mapping studies of resistance gene analogues (RGAs) provided evidence that they co-segregated with resistance markers.…”

Section: Introductionmentioning

confidence: 99%

Cloning and characterization of NBS-LRR resistance gene analogues of Musa spp. and their expression profiling studies against Pratylenchus coffeae

Backiyarani¹,

Uma²,

Arunkumar³

et al. 2013

Afr. J. Biotechnol.

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Resistance gene analogues (RGAs) were isolated from two banana cultivars viz., Karthobiumtham and Rose using degernate primers designed from the conserved motifs of different plant resistance genes. A total of 40 sequences were hit with various R genes, of which 20 sequences were having uninterrupted open reading frame (ORFs). Based on the conserved domains like P loop, internal kinase 2, kinase 3a and hydrophobic domain motifs of the deduced amino acid sequences were grouped as NBS-LRR class of resistant genes. The phylogentic analysis of RGAs showed that all the Musa RGAs are grouped under non-TIR branch and grouped into six distinct Musa RGA cluster. To investigate the expression profile of the RGAs, specific primers were designed for one representative RGA from each RGA cluster and it was found that C1 and C5 were induced upon root lesion nematode infection in the resistant (cv. Karthobiumtham) and not in susceptible (cv.Nendran) cultivar. C6 was expressed only in resistant cultivar not in susceptible one. But there was no change in the expression of C2 and C3 in both resistant and susceptible cultivars. These results indicate that in depth study on C1, and C5 RGAs will be helpful for further improvement of P. coffeae resistance in banana.

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Genetic mapping of plant disease resistance gene homologues using a minimal Brassica napus L. population

Cited by 22 publications

References 20 publications

Resistance gene analogs associated with Fusarium head blight resistance in wheat

Resistance gene analogs associated with Fusarium head blight resistance in wheat

Application of Molecular Biology for Identification of Virus Resistance Gene in Melon

Cloning and characterization of NBS-LRR resistance gene analogues of Musa spp. and their expression profiling studies against Pratylenchus coffeae

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