Genetic mapping of a fusarium wilt resistance gene in Brassica oleracea

Pu, Zi-jing; Shimizu, Mitsuru; Zhang, Yanju; Nagaoka, T.; Hayashi, Takeshi; Hori, Hidetaka; Matsumoto, Satoru; Fujimoto, Ryo; Okazaki, Keiichi

doi:10.1007/s11032-011-9665-8

Cited by 48 publications

(38 citation statements)

References 18 publications

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“…conglutinans categorized by race 1, provided by Dr. Kadota (National Agricultural Research Center for Tohoku Region, Japan), was used to prepare the inocula used in this study. Liquid inocula were prepared according to the method described by Pu et al (2012). Plants were grown in plastic dishes containing Murashige and Skoog (MS) agar medium supplemented with 1.0 % sucrose (pH 5.7) in growth chambers under a 16-h/8-h light/dark cycle at 25 °C for two weeks.…”

Section: Plant Materials and Inoculation Test Of Fusarium Oxysporum Fmentioning

confidence: 99%

Comparison of transcriptome profiles by Fusarium oxysporum inoculation between Fusarium yellows resistant and susceptible lines in Brassica rapa L.

et al. 2017

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Resistant and susceptible lines in Brassica rapa have different immune responses against Fusarium oxysporum inoculation. Fusarium yellows caused by Fusarium oxysporum f. sp. conglutinans (Foc) is an important disease of Brassicaceae; however, the mechanism of how host plants respond to Foc is still unknown. By comparing with and without Foc inoculation in both resistant and susceptible lines of Chinese cabbage (Brassica rapa var. pekinensis), we identified differentially expressed genes (DEGs) between the bulked inoculated (6, 12, 24, and 72 h after inoculation (HAI)) and non-inoculated samples. Most of the DEGs were up-regulated by Foc inoculation. Quantitative real-time RT-PCR showed that most up-regulated genes increased their expression levels from 24 HAI. An independent transcriptome analysis at 24 and 72 HAI was performed in resistant and susceptible lines. GO analysis using up-regulated genes at 24 HAI indicated that Foc inoculation activated systemic acquired resistance (SAR) in resistant lines and tryptophan biosynthetic process and responses to chitin and ethylene in susceptible lines. By contrast, GO analysis using up-regulated genes at 72 HAI showed the overrepresentation of some categories for the defense response in susceptible lines but not in the resistant lines. We also compared DEGs between B. rapa and Arabidopsis thaliana after F. oxysporum inoculation at the same time point, and identified genes related to defense response that were up-regulated in the resistant lines of Chinese cabbage and A. thaliana. Particular genes that changed expression levels overlapped between the two species, suggesting that they are candidates for genes involved in the resistance mechanisms against F. oxysporum.

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Section: Plant Materials and Inoculation Test Of Fusarium Oxysporum Fmentioning

confidence: 99%

Comparison of transcriptome profiles by Fusarium oxysporum inoculation between Fusarium yellows resistant and susceptible lines in Brassica rapa L.

et al. 2017

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“…Validations in an F 2 population revealed that the results for the molecular marker were consistent with the artificial inoculation results in 81% of the cases. Pu et al (2012) developed an SSR marker (i.e., KBrS003O1N10) associated with the CFW resistance gene at a genetic distance of 1.2 cM. Lv et al (2013) developed InDel marker A1, which produced results that were in agreement with those of artificial inoculations in 96 and 82% of the F 2 population and 40 inbred lines, respectively.…”

Section: Discussionmentioning

confidence: 98%

“…Pu et al (2012) and Lv et al (2013) have developed markers linked to the CFW resistance gene. Additionally, using doubled haploid (DH) and F 2 cabbage populations, Lv et al (2014a) fine-mapped a CFW resistance gene on an 84-kb interval, and identified a toll/interleukin-1 receptor-nucleotide biding site-leucine rich repeat (TIR–NBS–LRR) like gene (i.e., FOC1 ) as the candidate gene.…”

Section: Introductionmentioning

confidence: 99%

Rapid Introgression of the Fusarium Wilt Resistance Gene into an Elite Cabbage Line through the Combined Application of a Microspore Culture, Genome Background Analysis, and Disease Resistance-Specific Marker Assisted Foreground Selection

et al. 2017

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Cabbage is an economically important vegetable worldwide. Cabbage Fusarium Wilt (CFW) is a destructive disease that results in considerable yield and quality losses in cole crops. The use of CFW-resistant varieties is the most effective strategy to mitigate the effects of CFW. 01-20 is an elite cabbage line with desirable traits and a high combining ability, but it is highly susceptible to CFW. To rapidly transfer a CFW resistance gene into 01-20 plants, we used microspore cultures to develop 230 doubled haploid (DH) lines from a cross between 01-20 (highly susceptible) and 96-100 (highly resistant). One of the generated DH lines (i.e., D134) was highly resistant to CFW and exhibited a phenotypic performance that was similar to that of line 01-20. Therefore, D134 was applied as the resistance donor parent. We generated 24 insertion–deletion markers using whole genome resequencing data for lines 01-20 and 96-100 to analyze the genomic backgrounds of backcross (BC) progenies. Based on the CFW resistance gene FOC1, a simple sequence repeat (SSR) marker (i.e., Frg13) was developed for foreground selections. We screened 240 BC1 individuals and 280 BC2 individuals with these markers and assessed their phenotypic performance. The proportion of recurrent parent genome (PRPG) of the best individuals in BC1 and BC2 were 95.8 and 99.1%. Finally, a best individual designated as YR01-20 was identified from 80 BC2F1 individuals, with homozygous FOC1 allele and genomic background and phenotype almost the same as those of 01-20. Our results may provide a rapid and efficient way of improving elite lines through the combined application of microspore culture, whole-genome background analysis, and disease resistance-specific marker selection. Additionally, the cabbage lines developed in this study represent elite materials useful for the breeding of new CFW-resistant cabbage varieties.

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“…The type A single dominant resistance gene for Foc race 1 has been explored extensively in the last several years. The FW R gene FocBo1 was first mapped to linkage group seven using both BSA and QTL analysis by Pu et al 103 . Lv et al 104,105 generated a genetic linkage map based on a cabbage DH population and mapped the R gene FOC1 to a 1.8 cM interval between two adjacent InDel markers.…”

Section: Stem Rotmentioning

confidence: 99%

An update on the arsenal: mining resistance genes for disease management of Brassica crops in the genomic era

Fang

Yang

et al. 2020

Hortic Res

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Brassica species include many economically important crops that provide nutrition and health-promoting substances to humans worldwide. However, as with all crops, their production is constantly threatened by emerging viral, bacterial, and fungal diseases, whose incidence has increased in recent years. Traditional methods of control are often costly, present limited effectiveness, and cause environmental damage; instead, the ideal approach is to mine and utilize the resistance genes of the Brassica crop hosts themselves. Fortunately, the development of genomics, molecular genetics, and biological techniques enables us to rapidly discover and apply resistance (R) genes. Herein, the R genes identified in Brassica crops are summarized, including their mapping and cloning, possible molecular mechanisms, and application in resistance breeding. Future perspectives concerning how to accurately discover additional R gene resources and efficiently utilize these genes in the genomic era are also discussed.

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Genetic mapping of a fusarium wilt resistance gene in Brassica oleracea

Cited by 48 publications

References 18 publications

Comparison of transcriptome profiles by Fusarium oxysporum inoculation between Fusarium yellows resistant and susceptible lines in Brassica rapa L.

Comparison of transcriptome profiles by Fusarium oxysporum inoculation between Fusarium yellows resistant and susceptible lines in Brassica rapa L.

Rapid Introgression of the Fusarium Wilt Resistance Gene into an Elite Cabbage Line through the Combined Application of a Microspore Culture, Genome Background Analysis, and Disease Resistance-Specific Marker Assisted Foreground Selection

An update on the arsenal: mining resistance genes for disease management of Brassica crops in the genomic era

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