Genetic Mapping of 21 Genes on Mouse Chromosome 11 Reveals Disruptions in Linkage Conservation with Human Chromosome 5

Watkins‐Chow, Dawn E.; Buckwalter, Marion S.; Newhouse, Matthew M.; Lossie, Amy C.; Brinkmeier, Michelle L.; Camper, Sally A.

doi:10.1006/geno.1996.4532

Cited by 21 publications

(10 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The Gdf9 gene has two exons, precluding its discovery by exon trapping. Mouse Gdf9 has been previously mapped to chromosome 11 (29.5 cM) with no recombination seen between Il4 and Gdf9 in 1650 meioses (Watkins-Chow et al, 1997). The authors conclude that Il4 and Gdf9 map within 0.06 cM or 120 kb of each other.…”

Section: Comparative Mapping Of the Il-4 Cytokine Family Gene Clustermentioning

confidence: 82%

“…Il13 was genetically mapped to within 2.8 cM of Il3 (McKenzie et al, 1993). Recently, a high-resolution genetic map de-veloped using three interspecific backcross panels representing 1650 meioses suggested that all five mouse cytokine genes map within 0.3 cM, or approximately 600 kb (Watkins-Chow et al, 1997). It is not known how many additional genes residing on human chromosome 5q31 are linked to the cytokine cluster in other species.…”

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Identification of 40 Genes on a 1-Mb Contig around the IL-4 Cytokine Family Gene Cluster on Mouse Chromosome 11

et al. 2000

View full text Add to dashboard Cite

Section: Comparative Mapping Of the Il-4 Cytokine Family Gene Clustermentioning

confidence: 82%

Section: Introductionmentioning

confidence: 98%

Identification of 40 Genes on a 1-Mb Contig around the IL-4 Cytokine Family Gene Cluster on Mouse Chromosome 11

et al. 2000

View full text Add to dashboard Cite

“…These observations provide a new and clear example of a synteny group split into distinct conserved segments disrupted by a region of homology to a different chromosome (see, for instance, Ref. 21).…”

Section: Cdna Clones Encode a Polypeptide That Is Able To Hydroxylatementioning

confidence: 85%

Primary Structure, Tissue Distribution, and Chromosomal Localization of a Novel Isoform of Lysyl Hydroxylase (Lysyl Hydroxylase 3)

Valtavaara

Szpirer

et al. 1998

Journal of Biological Chemistry

115

113

View full text Add to dashboard Cite

We report characterization of a novel isoform of lysyl hydroxylase (lysyl hydroxylase 3, LH3). The cDNA clones encode a polypeptide of 738 amino acids, including a signal peptide. The amino acid sequence has a high overall identity with LH1 and LH2, the isoforms characterized earlier. Conserved regions are present in the carboxyl-terminal portion of the isoforms and also in the central part of the molecules. Histidine and asparagine residues, which are conserved in the other isoforms and are known to be required for enzymatic activity, are also conserved in the novel isoform. The gene for LH3 (PLOD3) has been assigned to human chromosome 7q36 and rat chromosome 12. Gene expression of LH3 is highly regulated in adult human tissues. A strong hybridization signal, corresponding to an mRNA 2.75 kilobases in size, is obtained in heart, placenta and pancreas on multiple tissue RNA blots. Expression of the cDNA in vitro results in the synthesis of a protein that hydroxylates lysyl residues in collagenous sequences in a nontriple helical conformation.Collagen is a group of structural proteins that are found essentially in all tissues. To date, 19 different collagen types have been identified that participate in the assembly of various kinds of polymers in the extracellular matrix (1-4). Collagens form the structural building blocks of tissues, but it is also clear that they have important regulatory functions. Some collagens form scaffolds that keep cells in place within tissues, connect tissues within an organ, and facilitate attachment and migration of cells. Collagens can also form links between cells and matrices, and some regulate the assembly and properties of the scaffold-forming collagens (5). Recent reports indicate that collagen can directly serve as a ligand for receptor tyrosine kinases and, as a consequence of binding to the receptor, induces a cascade of phosphorylation in cells (6, 7).The biosynthesis of collagens includes several posttranslational modifications, one of which is hydroxylation of lysyl residues. Hydroxylysine occurs in the Y position of the repeating X-Y-Gly triplets within the helical region of the collagen molecule. Hydroxylysine also occurs in the sequence of nonhelical telopeptide regions of some collagen molecules, when glycine is replaced by either serine or alanine (1, 2,8). The amount of the hydroxylysyl residues varies considerably between different collagen types. Variation is also found within the same collagen type in different tissues and even within the same tissues in different physiological and pathological states (1, 2,8). In addition to collagens, hydroxylysine residues are found in some other proteins, these include C1q complement, acetylcholinesterase, mannose-binding proteins, bovine conglutinin, and anglerfish somatostatin 28. It should be noted, however, that all these proteins, with the exception of somatostatin 28, contain collagenous domains in their structure (1). Hydroxylysyl residues may be glycosylated to form galactosylhydroxylysyl or glucosylgalactosylhydrox...

show abstract

“…However, whenever high resolution mapping was performed, the evidence suggests that small intrachromosomal rearrangements may be the rule rather than an exception, as summarized by Carver and Stubbs (1997). Similar examples are a 10-cM region of homology between human 5q and mouse 11, which is split into at least 4 different segments (Watkins-Chow et al 1997), and a 2.5 Mbp region of MMU16 homologous to HSA 22q that is rearranged into three different blocks (Lund et al 1999;Puech et al 1997). Thus, intrachromosomal rearrangements of the type uncovered here seem to be quite common once they can be reliably detected.…”

Section: Small Intrachromosomal Rearrangements-more Common Than Thoughtmentioning

confidence: 99%

Comparative Maps of Human 19p13.3 and Mouse Chromosome 10 Allow Identification of Sequences at Evolutionary Breakpoints

et al. 2000

View full text Add to dashboard Cite

A cosmid/bacterial artificial chromosome (BAC) contiguous (contig) map of human chromosome (HSA) 19p13.3 has been constructed, and over 50 genes have been localized to the contig. Genes and anonymous ESTs from ≈4000 kb of human 19p13.3 were placed on the central mouse chromosome 10 map by genetic mapping and pulsed-field gel electrophoresis (PFGE) analysis. A region of ∼2500 kb of HSA 19p13.3 is collinear to mouse chromosome (MMU) 10. In contrast, the adjacent ≈1200 kb are inverted. Two genes are located in a 50-kb region after the inversion on MMU 10, followed by a region of homology to mouse chromosome 17. The synteny breakpoint and one of the inversion breakpoints has been localized to sequenced regions in human <5 kb in size. Both breakpoints are rich in simple tandem repeats, including (TCTG)n, (CT)n, and (GTCTCT)n, suggesting that simple repeat sequences may be involved in chromosome breaks during evolution. The overall size of the region in mouse is smaller, although no large regions are missing. Comparing the physical maps to the genetic maps showed that in contrast to the higher-than-average rate of genetic recombination in gene-rich telomeric region on HSA 19p13.3, the average rate of recombination is lower than expected in the homologous mouse region. This might indicate that a hot spot of recombination may have been lost in mouse or gained in human during evolution, or that the position of sequences along the chromosome (telomeric compared to the middle of a chromosome) is important for recombination rates.

show abstract

Genetic Mapping of 21 Genes on Mouse Chromosome 11 Reveals Disruptions in Linkage Conservation with Human Chromosome 5

Cited by 21 publications

References 49 publications

Identification of 40 Genes on a 1-Mb Contig around the IL-4 Cytokine Family Gene Cluster on Mouse Chromosome 11

Identification of 40 Genes on a 1-Mb Contig around the IL-4 Cytokine Family Gene Cluster on Mouse Chromosome 11

Primary Structure, Tissue Distribution, and Chromosomal Localization of a Novel Isoform of Lysyl Hydroxylase (Lysyl Hydroxylase 3)

Comparative Maps of Human 19p13.3 and Mouse Chromosome 10 Allow Identification of Sequences at Evolutionary Breakpoints

Contact Info

Product

Resources

About