Genetic mapping and QTL analysis of agronomic traits in Indian Mucuna pruriens using an intraspecific F2 population

Abstract

Mucuna pruriens is a well-recognized agricultural and horticultural crop with important medicinal use. However, antinutritional factors in seed and adverse morphological characters have negatively affected its cultivation. To elucidate the genetic control of agronomic traits, an intraspecific genetic linkage map of Indian M. pruriens has been developed based on amplified fragment length polymorphism (AFLP) markers using 200 F₂ progenies derived from a cross between wild and cultivated genotypes. The resulting linkage map comprised 129 AFLP markers dispersed over 13 linkage groups spanning a total distance of 618.88 cM with an average marker interval of 4.79 cM. For the first time, three QTLs explaining about 6.05-14.77% of the corresponding total phenotypic variation for three quantitative (seed) traits and, eight QTLs explaining about 25.96% of the corresponding total phenotypic variation for three qualitative traits have been detected on four linkage groups. The map presented here will pave a way for mapping of genes/QTLs for the important agronomic and horticultural traits contrasting between the parents used in this study.

Quantitative Trait Loci (QTL) Analysis of Fruit and Agronomic Traits of Tropical Pumpkin (Cucurbita moschata) in an Organic Production System

“…Although we focused on molecular pumpkin breeding from conventional to organic farming in Hawai'i, this strategy can be applied to any crop whose main production occurs in a conventional setting and consumers demand an organically grown alternative. Indeed, we found that new technologies were as successful in this understudied system as in others [29,30]. Furthermore, our genetic and genomic information can be used to improve the genome assembly of pumpkin.…”