Genetic mapping and confirmation of the Rdm3 locus underlying resistance to southern stem canker in soybean

Abstract

Southern stem canker (SSC) in soybean [Glycine max (L.) Merrill], caused by the fungal pathogen Diaporthe aspalathi, is a major disease in the southern United States. It can cause up to 80% yield loss in severely infected fields. Plant introduction (PI) 398469, a Maturity Group VI soybean accession from South Korea, is one of six known sources of resistance to SSC. To determine the genetics of the resistance provided by PI 398469, F2:3 and F5‐derived recombinant inbred line (RIL) populations were developed from a cross with the susceptible breeding line G81‐2057. Both populations were phenotyped in the greenhouse using a modified toothpick method. The SSC‐resistant and susceptible DNA bulks consisting of 20 resistant and 18 susceptible families were created from the F2:3 population and genotyped using the Infinium SoySNP50K BeadChips for a bulked segregant analysis (BSA). Based on BSA results, 22 Kompetitive allele specific polymerase chain reaction (KASP) markers were designed to genotype the RIL population. Quantitative trait locus (QTL) analysis identified qRdm_14, on chromosome (Chr) 14 with an R2 of 62%. Linkage mapping in the F2:3 population confirmed the presence of a QTL on Chr 14. F5‐derived RILs derived from G81‐2057 × Crockett were phenotyped for SSC resistance and genotyped with the KASP markers to examine the relationship between Rdm3 from Crockett and qRdm_14 from PI 398469. Both PI 398469 and Crockett carried realleles at the Rdm3 locus for resistance to SSC. The QTL and KASP marker information could be used to screen breeding lines for resistance to SSC.

Current understanding of the Diaporthe/Phomopsis complex causing soybean stem canker: A focus on molecular aspects of the interaction

“…Both resistance loci, Rdm3 in both the cultivar Crockett and PI 398469 and Rdm? in PI 398469, are located on chromosome 14 and appear to be clustered with several genes encoding PRRs and NLRs (Maldonado dos Santos et al., 2019; Menke et al., 2023; Shearin, 2011). Several of these resistance loci have been mapped less than 14 cM from simple‐sequence repeats (SSRs) or single‐nucleotide polymorphism (SNP) markers that may be suitable for marker‐assisted selection in breeding programmes (Chiesa et al., 2009; Gilli et al., 2020; Shearin, 2011).…”

“…Both resistance loci, Rdm3 in both the cultivar Crockett and PI 398469 and Rdm? in PI 398469, are located on chromosome 14 and appear to be clustered with several genes encoding PRRs and NLRs (Maldonado dos Santos et al, 2019;Menke et al, 2023;Shearin, 2011). Several of these resistance loci have been mapped…”