Genetic Mapping and Confirmation of Quantitative Trait Loci for Seed Protein and Oil Contents and Seed Weight in Soybean

Abstract

Demand for soybean [Glycine max (L.) Merr.] meal has increased worldwide and soybean importers often offer premiums for soybean containing higher contents of protein and oil. Objectives were to detect quantitative trait loci (QTL) associated with soybean seed protein, oil, and seed weight in a soybean mapping population and confirm detected QTL across genetic backgrounds and environments. Two populations of 216 and 156 recombinant inbred lines were developed from Magellan × PI 438489B and Magellan × PI 567516C crosses, respectively, and grown in two Missouri environments in 2008. More than 900 simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers were used for mapping in each population. Across environments and genetic backgrounds, we have identified seven QTL for protein, six for oil, and four for seed weight. Two QTL were detected in common for protein and oil, one on chromosome (Chr.) 5 and another on Chr. 6. Additionally, we have detected one new seed weight QTL on Chr. 6, in the same region of protein and oil QTL. Confirmed protein and oil QTL on Chrs. 5 and 6 may be important targets to find candidate genes involved in modification of protein and oil contents and seed weight using genetic and genomic approaches. Also, SSR and SNP markers closely associated with these QTL can be useful for marker‐assisted selection.

Correlations of seed traits with tofu texture in 48 soybean cultivars and breeding lines

“…This is not unexpected as there is a long-established negative correlation between oil and protein content in soybean seed with correlation coefficients ranging from À0.18 to À0.67 (e.g. Johnson and Bernard 1962, Wilcox 1998, Yaklich et al 2002, Shi et al 2010, Pathan et al 2013). This study also reported a strong, negative correlation of À0.82 between seed oil and protein content.…”

“…, Pathan et al. ). This study also reported a strong, negative correlation of −0.82 between seed oil and protein content.…”

Agronomic Evaluation of a High Protein Allele from PI407788A on Chromosome 15 across Two Soybean Backgrounds

“…The QTL from PI407788A was mapped by Kim et al (2015), but multiple seed composition QTL have been mapped to this location prior to and following that study (Diers et al, 1992;Sebolt et al, 2000;Fasoula et al, 2004;Pathan et al, 2013;Bandillo et al, 2015;Phansak et al, 2016;Qi et al, 2016). The QTL from PI407788A was mapped by Kim et al (2015), but multiple seed composition QTL have been mapped to this location prior to and following that study (Diers et al, 1992;Sebolt et al, 2000;Fasoula et al, 2004;Pathan et al, 2013;Bandillo et al, 2015;Phansak et al, 2016;Qi et al, 2016).…”

QTL mapping for yield components and agronomic traits in a Brazilian soybean population

“…QTL are reported for traits related to weight and size of the seeds (Liu et al 2011, Xu et al 2011, Han et al 2012, Sun et al 2012, Hu et al 2013, Liu et al 2013, Pathan et al 2013, Yesudas et al 2013, Kato et al 2014. Moreover, at least six QTL have been reported for grain yield, specifically (Palomeque et al 2009, Sebastian et al 2010, Kim et al 2012, Fox et al 2015, besides the several QTL mapped for other grain yield components from different populations , Guzman et al 2007, Zhang et al 2010, Liu et al 2011, Liu et al 2013.…”