Genetic Map of Triticale Integrating Microsatellite, DArT and SNP Markers

Abstract

Triticale (×Triticosecale Wittm) is an economically important crop for fodder and biomass production. To facilitate the identification of markers for agronomically important traits and for genetic and genomic characteristics of this species, a new high-density genetic linkage map of triticale was constructed using doubled haploid (DH) population derived from a cross between cultivars ‘Hewo’ and ‘Magnat’. The map consists of 1615 bin markers, that represent 50 simple sequence repeat (SSR), 842 diversity array technology (DArT), and 16888 DArTseq markers mapped onto 20 linkage groups assigned to the A, B, and R genomes of triticale. No markers specific to chromosome 7R were found, instead mosaic linkage group composed of 1880 highly distorted markers (116 bins) from 10 wheat chromosomes was identified. The genetic map covers 4907 cM with a mean distance between two bins of 3.0 cM. Comparative analysis in respect to published maps of wheat, rye and triticale revealed possible deletions in chromosomes 4B, 5A, and 6A, as well as inversion in chromosome 7B. The number of bin markers in each chromosome varied from 24 in chromosome 3R to 147 in chromosome 6R. The length of individual chromosomes ranged between 50.7 cM for chromosome 2R and 386.2 cM for chromosome 7B. A total of 512 (31.7%) bin markers showed significant (P < 0.05) segregation distortion across all chromosomes. The number of 8 the segregation distorted regions (SDRs) were identified on 1A, 7A, 1B, 2B, 7B (2 SDRs), 5R and 6R chromosomes. The high-density genetic map of triticale will facilitate fine mapping of quantitative trait loci, the identification of candidate genes and map-based cloning.

Changes in phenolic acid abundance involved in low temperature and Microdochium nivale (Samuels and Hallett) cross-tolerance in winter triticale (x Triticosecale Wittmack)

“…The 92 DH lines were preliminarily tested in controlled conditions for their susceptibility to M. nivale, and DH1 and DH92 were selected for the present experiments due to the best and the worst performance in the test. The mapping of the population of 92 DH lines and the parental 'Hewo' and 'Magnat' lines with DArT and SSR markers confirmed their homozygotic condition (Tyrka et al 2015) that makes this set of material especially suited for further experiments.…”

DArT Markers Effectively Target Gene Space in the Rye Genome

“…The first high-throughput genotyping technology was established for rye in 2009 with the development of a 1520-clone DArT genotyping panel (Bolibok-Bragoszewska et al, 2009). Since then, it was successfully applied for high density genetic map construction, both in rye and triticale (Bolibok-Bragoszewska et al, 2009; Milczarski et al, 2011; Tyrka et al, 2011, 2015), genome-wide germplasm characterization (Bolibok-Bragoszewska et al, 2014), QTL/gene mapping (Stojałowski et al, 2011; Miedaner et al, 2012; Myśków et al, 2012; Milczarski et al, 2016), and genomic selection (Wang et al, 2014, 2015; Schulthess et al, 2015). Following transcriptome sequencing a Rye5K SNP genotyping panel was developed and used for construction of high density transcript map (Haseneyer et al, 2011; Martis et al, 2013).…”

Participation of Wheat and Rye Genome in Drought Induced Senescence in Winter Triticale (X Triticosecale Wittm.)

“…The mapping population we used involved 92 lines of DH population 'Hewo' × 'Magnat' [27]. The genetic map composed of 1615 bin markers representing 13,402 DArTseq, 842 DArT, and 50 SSR markers used for QTL calculations [19]. The genetic map covered 4907 cM with a mean distance between two bins of 3.0 cM.…”

“…For all QTLs delimited by LOD values corresponding to a p-value of 0.05, redundant markers and corresponding DArTseq sequences were retrieved [19]. Selected sequences of rye DarT markers from the QTL regions were recently released [36] and obtained from the National Center for Biotechnology Information (NCBI).…”

“…There are genetic maps of triticale, wheat and rye that have been developed using SSR (simple sequence repeat), DArT (Diversity Arrays Technology), AFLP (Amplified Fragment Length Polymorphism), RAPD (Random Amplified Polymorphic DNA), RAMP (Random Amplified Microsatellite Polymorphic) and SNP (Single-Nucleotide Polymorphism) markers [19][20][21][22][23][24][25].…”