Genetic Manipulations of the Hyperthermophilic Piezophilic Archaeon Thermococcus barophilus

Thiel, Axel; Michoud, Grégoire; Moalic, Yann; Flament, Didier; Jebbar, Mohamed

doi:10.1128/aem.00084-14

Cited by 35 publications

(35 citation statements)

References 30 publications

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“…The most recognized genetic systems for piezophilic Archaea have been developed in the hyperthermo-piezophiles Pyrococcus abyssi GE5 [37], P. yayanosii [38] and Thermococcus barophilus MP [39]. Because most isolated piezophilic archaea belong to the order Thermococcales, the genetic systems for piezophilic archaea are often modified from non-piezophilic Thermococcales, for example, Thermococcus kodakaraensis KOD1.…”

Section: Genetic Engineering Of Piezophilic Microorganismsmentioning

confidence: 99%

Current developments in marine microbiology: high-pressure biotechnology and the genetic engineering of piezophiles

Zhang

Bartlett

et al. 2015

Current Opinion in Biotechnology

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Section: Genetic Engineering Of Piezophilic Microorganismsmentioning

confidence: 99%

Current developments in marine microbiology: high-pressure biotechnology and the genetic engineering of piezophiles

Zhang

Bartlett

et al. 2015

Current Opinion in Biotechnology

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“…Therefore, plasmid curing could remarkably reduce the energy burden in archaeal thermophiles. We note that many archaeal thermophiles harbor plasmids, such as S. solfataricus P2 (carrying pSSVi) and T. barophilus MP (carrying pTBMP1), which have been studied as model acidophilic hyperthermophiles [38] and piezophilic hyperthermophiles [39], respectively. Even if these thermophiles harbor a single copy plasmid, our results suggest that plasmid curing can improve their performance in terms of the cell density and protein productivity.…”

Section: Discussionmentioning

confidence: 99%

Plasmid Curing is a Promising Approach to Improve Thermophiles for Biotechnological Applications: Perspectives in Archaea

Mizuno¹,

Ohshiro²,

Suzuki³

2017

Archaea - New Biocatalysts, Novel Pharmaceuticals and Various Biotechnological Applications

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Thermophiles are atractive as host cells for microbial processes to produce or degrade various compounds. In these applications, it is often desirable to improve the properties of thermophiles, such as their growth rate, cell density, and protein productivity, although this is rarely achieved because of the lack of general approaches. In this chapter, we describe the elimination of the pHTA426 plasmid from a moderate thermophile, Geobacillus kaustophilus HTA426, and its efects on the microbial properties. This process, called plasmid curing, was simply achieved using a DNA intercalator and conirmed by phenotypic and genotypic analyses. Of note, pHTA426 curing had beneicial efects on diverse properties, probably because of the reduced energy burden in terms of plasmid replication at high temperatures. The result suggests that plasmid curing is a simple and versatile approach for improving thermophiles. In particular, this approach may be efective for archaeal thermophiles because they grow at much higher temperatures and could have the greater energy burden on plasmid replication. Data mining has also shown that plasmids are distributed in archaeal thermophiles. This chapter provides a new tip for improving archaeal thermophiles, thereby increasing the opportunities for their use in various biotechnological applications.

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“…To this end, we discriminated the sedimentation profile behaviours of endogenous aRNase J, ASH-Ski2 and Rrp41 in two Thermococcales organisms, Thermococcus barophilus (Tba) and P. abyssi (Pab) by doing ultracentrifugation of whole-cell extracts on continuous 10-30% sucrose density gradient (Figure 6a; Appendix Figure S5a, respectively). Since to this date, P. abyssi cannot be genetically modified, the T. barophilus strain was used as a genetically tractable model (51,52) to assess the sedimentation profile of endogenous aRNase J in absence of ASH-Ski2 and vice-versa.…”

Section: Arnase J and Ash-ski2 And The Rna Exosome Co-sediment In Sucmentioning

confidence: 99%

RNA processing machineries in Archaea: the 5’-3’ exoribonuclease aRNase J of the β-CASP family is engaged specifically with the helicase ASH-Ski2 and the 3’-5’ exoribonucleolytic RNA exosome machinery

Phung

Etienne

Batista

et al. 2019

Preprint

Self Cite

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A network of RNA helicases, endoribonucleases, and exoribonucleases regulates the quantity and quality of cellular RNAs. To date, mechanistic studies focused on bacterial and eukaryal systems due to the challenge of identifying the main drivers of RNA decay and processing in Archaea. Here, our data support that aRNase J, a 5'-3' exoribonuclease of the β-CASP family conserved in Euryarchaea, engages specifically with a Ski2-like helicase and the RNA exosome to potentially exert control over RNA surveillance, and that this occurs in the vicinity of the ribosome. Proteomic landscapes and direct protein-protein interaction analyses demonstrated that aRNase J interplay with ASH-Ski2 and the Csl4 cap exosome subunit. These in vitro data are strengthened by our phylogenomic studies showing a taxonomic co-distribution of aRNase J and ASH-Ski2 among the archaeal phylogeny. Finally, our T. barophilus whole-cell extract fractionation experiments provide evidences that an aRNase J/ASH-Ski2 complex might exist in vivo and hint at an association of aRNase J with the ribosome or polysomes that is stressed in absence of ASH-Ski2. While aRNase J homologues are found among bacteria, the RNA exosome and the Ski2-like RNA helicase have eukaryotic homologues, underlining the mosaic aspect of archaeal RNA machines. Altogether, these results suggest, for the first time, a fundamental role of β-CASP RNase/helicase complex in archaeal RNA metabolism. Finally, our results position aRNase J at the junction of RNA surveillance and translation processes, thus opening new perspectives and evolutionary scenario on RNA processing players in Archaea.

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Genetic Manipulations of the Hyperthermophilic Piezophilic Archaeon Thermococcus barophilus

Cited by 35 publications

References 30 publications

Current developments in marine microbiology: high-pressure biotechnology and the genetic engineering of piezophiles

Current developments in marine microbiology: high-pressure biotechnology and the genetic engineering of piezophiles

Plasmid Curing is a Promising Approach to Improve Thermophiles for Biotechnological Applications: Perspectives in Archaea

RNA processing machineries in Archaea: the 5’-3’ exoribonuclease aRNase J of the β-CASP family is engaged specifically with the helicase ASH-Ski2 and the 3’-5’ exoribonucleolytic RNA exosome machinery

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