Genetic manipulation of phages for therapy using BRED

Payaslián, Florencia; Gradaschi, Victoria; Piuri, Mariana

doi:10.1016/j.copbio.2020.09.005

Cited by 19 publications

(14 citation statements)

References 54 publications

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“… 71 With about half of the sequenced bacteria being lysogens, temperate phages could be easier to find than isolating lytic phages from nature. 72 …”

Section: Application Of Phages In Bacterial Biofilm Destructionmentioning

confidence: 99%

“…71 With about half of the sequenced bacteria being lysogens, temperate phages could be easier to find than isolating lytic phages from nature. 72 In a recent study, endolysin PM-477 of the type 1,4-beta-N-acetylmuramidase encoded on Gardnerella prophages when recombinantly expressed, demonstrated strong bactericidal activity against four different Gardnerella species. By domain shuffling, several engineered endolysins with 10-fold higher bactericidal activity than any wild-type enzyme was generated.…”

Section: Dovepressmentioning

confidence: 99%

See 1 more Smart Citation

Bacterial Biofilm Destruction: A Focused Review On The Recent Use of Phage-Based Strategies With Other Antibiofilm Agents

Amankwah¹,

Abdella²,

Kassa³

2021

NSA

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Biofilms are bacterial communities that live in association with biotic or abiotic surfaces and enclosed in an extracellular polymeric substance. Their formation on both biotic and abiotic surfaces, including human tissue and medical device surfaces, pose a major threat causing chronic infections. In addition, current antibiotics and antiseptic agents have shown limited ability to completely remove biofilms. In this review, the authors provide an overview on the formation of bacterial biofilms and its characteristics, burden and evolution with phages. Moreover, the most recent possible use of phages and phage-derived enzymes to combat bacteria in biofilm structures is elucidated. From the emerging results, it can be concluded that despite successful use of phages and phage-derived products in destroying biofilms, they are mostly not adequate to eradicate all bacterial cells. Nevertheless, a combined therapy with the use of phages and/or phage-derived products with other antimicrobial agents including antibiotics, nanoparticles, and antimicrobial peptides may be effective approaches to remove biofilms from medical device surfaces and to treat their associated infections in humans.

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“… 71 With about half of the sequenced bacteria being lysogens, temperate phages could be easier to find than isolating lytic phages from nature. 72 …”

Section: Application Of Phages In Bacterial Biofilm Destructionmentioning

confidence: 99%

Section: Dovepressmentioning

confidence: 99%

Bacterial Biofilm Destruction: A Focused Review On The Recent Use of Phage-Based Strategies With Other Antibiofilm Agents

Amankwah¹,

Abdella²,

Kassa³

2021

NSA

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“…Their biotechnological potential is already being exploited in a variety of fields, with uses including therapy, bacteria typing and detection, surface disinfection, food decontamination, drug delivery, and vaccine development. A definitive advantage is that phage protein manipulations and regulation are easily social accepted, and these engineered polypeptides can play a major role in scientific discoveries [36].…”

Section: Exploiting Bacteriophage Proteomesmentioning

confidence: 99%

The Use of Bacteriophages in Biotechnology and Recent Insights into Proteomics

et al. 2022

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Phages have certain features, such as their ability to form protein–protein interactions, that make them good candidates for use in a variety of beneficial applications, such as in human or animal health, industry, food science, food safety, and agriculture. It is essential to identify and characterize the proteins produced by particular phages in order to use these viruses in a variety of functional processes, such as bacterial detection, as vehicles for drug delivery, in vaccine development, and to combat multidrug resistant bacterial infections. Furthermore, phages can also play a major role in the design of a variety of cheap and stable sensors as well as in diagnostic assays that can either specifically identify specific compounds or detect bacteria. This article reviews recently developed phage-based techniques, such as the use of recombinant tempered phages, phage display and phage amplification-based detection. It also encompasses the application of phages as capture elements, biosensors and bioreceptors, with a special emphasis on novel bacteriophage-based mass spectrometry (MS) applications.

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“…Since then, it has been suggested that the administration of these viruses could be used to treat bacterial infections. Initial results with phage therapy (PT) were promising; however, its application was limited owing to the discovery of penicillin in 1928 as well as the large-scale production of antibiotics in 1940 [1][2][3][4]. In recent years, the use of PT has recommenced owing to the increased bacterial resistance to antibiotics, which can arise because of mutations or be acquired by the acquisition of resistance-conferring genes via horizontal gene transfer (HGT).…”

Section: Introductionmentioning

confidence: 99%

“…The World Health Organization (WHO) has listed critical priority pathogens that require new antibiotics or therapeutic alternatives; these pathogens include multidrugresistant bacteria that are especially dangerous in hospitals and nursing homes and for patients who need to be treated with ventilators and intravenous catheters [9]. According to the Organization for Economic Cooperation and Development (OECD), in Europe, North America, and Australia alone, 2.4 million people could die between now and 2050 if current bacterial resistance rates continue [10], which evidences that bacterial resistance poses a global public health problem [2][3][4][5][6]11]. Likewise, the discovery and development of new antibiotics have decreased because of the large amount of time and money required, resulting in increasingly difficult clinical management of infections and, in some cases, infections that are impossible to treat [9,11,12].…”

Section: Introductionmentioning

confidence: 99%

Phage Therapy in the Era of Multidrug Resistance in Bacteria: A Systematic Review

Aranaga

Pantoja

Martínez

et al. 2022

IJMS

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Bacteriophages offer an alternative for the treatment of multidrug-resistant bacterial diseases as their mechanism of action differs from that of antibiotics. However, their application in the clinical field is limited to specific cases of patients with few or no other alternative therapies. This systematic review assesses the effectiveness and safety of phage therapy against multidrug-resistant bacteria through the evaluation of studies published over the past decade. To that end, a bibliographic search was carried out in the PubMed, Science Direct, and Google Scholar databases. Of the 1500 studies found, 27 met the inclusion criteria, with a total of 165 treated patients. Treatment effectiveness, defined as the reduction in or elimination of the bacterial load, was 85%. Except for two patients who died from causes unrelated to phage therapy, no serious adverse events were reported. This shows that phage therapy could be an alternative treatment for patients with infections associated with multidrug-resistant bacteria. However, owing to the phage specificity required for the treatment of various bacterial strains, this therapy must be personalized in terms of bacteriophage type, route of administration, and dosage.

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Genetic manipulation of phages for therapy using BRED

Cited by 19 publications

References 54 publications

Bacterial Biofilm Destruction: A Focused Review On The Recent Use of Phage-Based Strategies With Other Antibiofilm Agents

Bacterial Biofilm Destruction: A Focused Review On The Recent Use of Phage-Based Strategies With Other Antibiofilm Agents

The Use of Bacteriophages in Biotechnology and Recent Insights into Proteomics

Phage Therapy in the Era of Multidrug Resistance in Bacteria: A Systematic Review

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