Genetic manipulation of pea (Pisum sativum L.) with Arabidopsisˈs heat shock factor HsfA1d improves ROS scavenging system to confront thermal stress

Shah, Zamarud; Iqbal, Arshad; Khan, Fahim Ullah; Khan, Hidayat Ullah; Durrani, Faran; Ahmad, Muhammad Zulfiqar

doi:10.1007/s10722-020-00966-9

Cited by 11 publications

(10 citation statements)

References 27 publications

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“…Knockdown of HSFA1 genes in Arabidopsis led to a reduced induction of many HS-responsive genes, as a result plants demonstrated HS susceptible phenotypes [52,53]. The thermo-tolerance conferred by Arabidopsis HSFA1d was further confirmed in a recent study in pea [54], where transformant pea plants with this Arabidopsis HSF was more heat tolerant than its wild type due to the increased antioxidant enzyme activity and reduced hydrogen per oxide. Another study in Arabidopsis concluded that HSFA3 was also an important HS-responsive TF, because knockout or knockdown mutation of HSFA3 resulted in reduced expression of putative target HSP genes during HS [55].…”

Section: Regulatory Importance Of Hsfa3 and Hsfb2 In Hsrmentioning

confidence: 96%

Identification of heat responsive genes in pea stipules and anthers through transcriptional profiling

et al. 2021

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Field pea (Pisum sativum L.), a cool-season legume crop, is known for poor heat tolerance. Our previous work identified PR11-2 and PR11-90 as heat tolerant and susceptible lines in a recombinant inbred population. CDC Amarillo, a Canadian elite pea variety, was considered as another heat tolerant variety based on its similar field performance as PR11-2. This study aimed to characterize the differential transcription. Plants of these three varieties were stressed for 3 h at 38°C prior to self-pollination, and RNAs from heat stressed anthers and stipules on the same flowering node were extracted and sequenced via the Illumina NovaSeq platform for the characterization of heat responsive genes. In silico results were further validated by qPCR assay. Differentially expressed genes (DEGs) were identified at log2 |fold change (FC)| ≥ 2 between high temperature and control temperature, the three varieties shared 588 DEGs which were up-regulated and 220 genes which were down-regulated in anthers when subjected to heat treatment. In stipules, 879 DEGs (463/416 upregulation/downregulation) were consistent among varieties. The above heat-induced genes of the two plant organs were related to several biological processes i.e., response to heat, protein folding and DNA templated transcription. Ten gene ontology (GO) terms were over-represented in the consistently down-regulated DEGs of the two organs, and these terms were mainly related to cell wall macromolecule metabolism, lipid transport, lipid localization, and lipid metabolic processes. GO enrichment analysis on distinct DEGs of individual pea varieties suggested that heat affected biological processes were dynamic, and variety distinct responses provide insight into molecular mechanisms of heat-tolerance response. Several biological processes, e.g., cellular response to DNA damage stimulus in stipule, electron transport chain in anther that were only observed in heat induced PR11-2 and CDC Amarillo, and their relevance to field pea heat tolerance is worth further validation.

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Section: Regulatory Importance Of Hsfa3 and Hsfb2 In Hsrmentioning

confidence: 96%

Identification of heat responsive genes in pea stipules and anthers through transcriptional profiling

et al. 2021

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“…Knockdown of HSFA1 genes in Arabidopsis led to a reduced induction of many HS-responsive genes, as a result plants demonstrated HS susceptible phenotypes [47,48]. The thermo-tolerance conferred by Arabidopsis HSFA1d was further confirmed in a recent study in pea [49], where transformant pea plants with this Arabidopsis HSF was more heat tolerant than its wild type due to the increased antioxidant enzyme activity and reduced hydrogen per oxide. Another study in Arabidopsis concluded that HSFA3 was also an important HS-responsive TF, because knockout or knockdown mutation of HSFA3 resulted in reduced expression of putative target HSP genes during HS [50].…”

Section: Regulatory Importance Of Hsfa3 and Hsfb2 In Heat Responsementioning

confidence: 96%

Identification of heat responsive genes in pea stipules and anthers through transcriptional profiling

Huang

Kishore

Lachagari

et al. 2021

Preprint

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Field pea (Pisum sativum L.), a cool-season legume crop, is known for poor heat tolerance. Our previous work identified PR11-2 and PR11-90 as heat tolerant and susceptible lines in a recombinant inbred population. CDC Amarillo, a Canadian elite pea variety, was considered as another heat tolerant variety based on its similar field performance as PR11-2. This study aimed to characterize the differential transcription. Plants of these three varieties were stressed for 3h at 38°C prior to self-pollination, and RNAs from heat stressed anthers and stipules on the same flowering node were extracted and sequenced via the Illumina NovaSeq platform for the characterization of heat responsive genes. In silico results were further validated by qPCR assay. Differentially expressed genes (DEGs) were identified at log2 fold change, the three varieties shared 588 DEGs which were up-regulated and 220 genes which were down-regulated in anthers when subjected to heat treatment. In stipules, 879 DEGs (463/416 upregulation/downregulation) were consistent among varieties. The above heat-induced genes of the two plant organs were related to several biological processes i.e., response to heat, protein folding and DNA templated transcription. Ten gene ontology (GO) terms were over-represented in the consistently down-regulated DEGs of the two organs, and these terms were mainly related to cell wall macromolecule metabolism, lipid transport, lipid localization, and lipid metabolic processes. GO enrichment analysis on distinct DEGs of individual pea varieties suggested that heat affected biological processes were dynamic, and variety distinct responses provide insight into molecular mechanisms of heat-tolerance response. Several biological processes, e.g., cellular response to DNA damage stimulus in stipule, electron transport chain in anther that were only observed in heat induced PR11-2 and CDC Amarillo, and their relevance to field pea heat tolerance is worth further validation.

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“…Heat shock proteins (HSPs) are evolutionarily conserved chaperones that prevent protein misfolding and denaturation induced by external stresses including HS ( Will et al., 2017 ). First discovered in 1962 ( Kregel, 2002 ), the regulation of HSPs/heat shock factors (HSFs) are known to govern HS tolerance in peas ( Shah et al., 2020 ). Some plants synthesize up to 30-40 HSPs in response to HS ( Mansfield and Key, 1987 ; Al-Whaibi, 2011 ) and it is assumed that the diversity of these proteins represents an adaptation to HS.…”

Section: Impact On Physiological Biochemical Traits and Molecular Cha...mentioning

confidence: 99%

“…Mitochondrial nucleoside diphosphate kinase ( mtNDPK ) is reportedly involved in HS response through its interaction with a novel heat shock inducible 86-kD protein in peas ( Escobar Galvis et al., 2001 ). Improved HS tolerance in peas was reported by incorporating ‘ HsfA1d ’ isolated from Arabidopsis thaliana ( Shah et al., 2020 ). Recently, Huang et al.…”

Section: Impact On Physiological Biochemical Traits and Molecular Cha...mentioning

confidence: 99%

Heat stress tolerance in peas (Pisum sativum L.): Current status and way forward

Devi

Sagar²,

Mishra³

et al. 2023

Front. Plant Sci.

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In the era of climate change, the overall productivity of pea (Pisum sativum L.) is being threatened by several abiotic stresses including heat stress (HS). HS causes severe yield losses by adversely affecting several traits in peas. A reduction in pod yield has been reported from 11.1% to 17.5% when mean daily temperature increase from 1.4 to 2.2°C. High-temperature stress (30.5-33°C) especially during reproductive phase is known to drastically reduce both seed yield and germination. HS during germination and early vegetative stage resulted in poor emergence and stunted plant growth along with detrimental effects on physiological functions of the pea plant. To combat HS and continue its life cycle, plants use various defense strategies including heat escape, avoidance or tolerance mechanisms. Ironically, the threshold temperatures for pea plant and its responses are inconsistent and not yet clearly identified. Trait discovery through traditional breeding such as semi leaflessness (afila), upright growing habit, lodging tolerance, lower canopy temperature and small seeded nature has highlighted their utility for greater adaptation under HS in pea. Screening of crop gene pool and landraces for HS tolerance in a targeted environment is a simple approach to identify HS tolerant genotypes. Thus, precise phenotyping using modern phenomics tools could lead to increased breeding efficiency. The NGS (next generation sequencing) data can be associated to find the candidate genes responsible for the HS tolerance in pea. In addition, genomic selection, genome wide association studies (GWAS) and marker assisted selection (MAS) can be used for the development of HS tolerant pea genotypes. Additionally, development of transgenics could be an alternative strategy for the development of HS tolerant pea genotypes. This review comprehensively covers the various aspects of HS tolerance mechanisms in the pea plant, screening protocols, omic advances, and future challenges for the development of HS tolerant genotypes.

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Genetic manipulation of pea (Pisum sativum L.) with Arabidopsisˈs heat shock factor HsfA1d improves ROS scavenging system to confront thermal stress

Cited by 11 publications

References 27 publications

Identification of heat responsive genes in pea stipules and anthers through transcriptional profiling

Identification of heat responsive genes in pea stipules and anthers through transcriptional profiling

Identification of heat responsive genes in pea stipules and anthers through transcriptional profiling

Heat stress tolerance in peas (Pisum sativum L.): Current status and way forward

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