Genetic manipulation of Agrobacterium tumefaciens strains to improve transformation of recalcitrant plant species

Gelvin, Stanton B.; Liu, Chang-Nong

doi:10.1007/978-94-011-0511-8_6

Cited by 14 publications

(12 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…DNA was extracted from young leaves using a standard cetyltrimethylammonium bromide (CTAB) method [11]. The concentration of the DNA samples was determined with a spectrophotometer and DNA samples were diluted to 50 ng/μl for analysis using SSR markers.…”

Section: Dna Extraction and Ssr Analysismentioning

confidence: 99%

Field Performance of Five Soybean Mutants Under Drought Stress Conditions and Molecular Analysis Using SSR Markers

Yuliasti

Reflinur

2017

Atom Indo.

View full text Add to dashboard Cite

The objectives of this research were to evaluate (1) the performance of soybean mutant lines under drought stress conditions, and (2) the genetic diversity and relationship among the mutant lines using SSR markers. The field evaluation was conducted during the dry season of 2011 and 2012 at the experimental Farm of Mataram University, West Nusa Tenggara, Indonesia. The field experiment was set up in a randomized block design. Ten mutant lines and two control varieties were evaluated in four replications. Genetic distance among evaluated lines were determined based on allelic diversity analysis using 40 simple sequence repeat (SSR) loci. Under drought stress conditions, two mutant lines, Kdl3 and Kdl8, showed a better performance compared to the other ones. The high yielding mutant lines were Kdl3 and Kdl8, which yielded 1.75 t ha-1 and 1.69 t ha-1, respectively, compared to the parent and national control, Panderman 1.43 t ha-1 and Muria 1.32 t ha-1. These mutant lines required 30.75 to 32 days to flower and 79.75 to 83.75 day to harvest with relatively short plant height 28.25 and 23.35 cm respectively. Those mutant characters were better than those of the other three mutants, the original parents, and the control soybean species. Since the evaluated soybean mutant lines yielded more under drought stress conditions than the standard varieties, they can be used and registered as drought-tolerant soybean mutants. Moreover, the evaluated soybean accessions showed a wide genetic distance. The accessions were clustered into two groups according to their genetic background, namely group I (the Panderman with three mutant lines) and group II (the Muria with two mutant lines). Twenty-three out of 40 evaluated SSR loci,

show abstract

Section: Dna Extraction and Ssr Analysismentioning

confidence: 99%

Field Performance of Five Soybean Mutants Under Drought Stress Conditions and Molecular Analysis Using SSR Markers

Yuliasti

Reflinur

2017

Atom Indo.

View full text Add to dashboard Cite

show abstract

“…Agrobacterium cells were grown for inoculation of plant tissues in a minimal induction medium containing 100 mM acetosyringone (Gelvin and Liu, 1994). An overnight bacterial culture grown in YEP medium was Maps (not to scale) of plasmid pCry1Ab3 containing the synthetic cry1Ab3 gene (a) and plasmid pCry1Ia3 containing the wild-type cry1Ia3 gene (b).…”

Section: Methodsmentioning

confidence: 99%

Development of transgenic cabbage (Brassica oleracea var. Capitata) for insect resistance by Agrobacterium tumefaciens-mediated transformation

Jin

Liu

Tabashnik

et al. 2000

In Vitro Cell.Dev.Biol.-Plant

View full text Add to dashboard Cite

Transgenic head cabbage (Brassica oleracea var. capitata), resistant to diamondback moth (Plutella xylostella) larvae, was developed through Agrobacterium tumefaciens-mediated transformation with Bacillus thuringiensis (Bt) cry genes using a modified procedure. Factors important for transformation included cabbage cultivar; preculture and coculture of explants on a callus initiation medium; use of appropriate amount; and delay in initial application of selective agents. A total of 15 independent transformed lines with over 100 plants were obtained from several transformation experiments, representing an overall transformation efficiency of ,1%. Cabbage plants transformed with a synthetic Bt gene, cry1Ab3, were all resistant to larvae of the diamondback moth, whereas all plants transgenic for cry1Ia3, a wild-type Bt gene, were susceptible. As a first step towards testing the hypothesis that reduced exposure of Bt to target insects would delay the evolution of insect resistance to Bt, cry1Ab3 expression was put under the transcriptional control of the soybean woundinducible vspB promoter and transgenic cabbage was obtained. Insect bioassay showed that such plants were all resistant to diamondback moth even without induction for the expression of Bt.

show abstract

“…Agrobacterium-mediated transformation A 10 ml overnight culture of Agrobacterium in L-broth was pelleted and resuspended in 40 ml AB minimal media (Gelvin & Liu 1994) plus 200 mM acetosyringone and cultured for 4 h (shaking at 288C, A 600 00.5Á0.6). Target explants were soaked in the Agrobacterium solution for 10 min, blotted dry on sterile filter paper then cultured on selection-free media for 48 h before being transferred to regeneration media plus ticarcillan † (500 ppm) and geneticin (10 ppm) for selection of transgenic loci.…”

Section: Biolistic-mediated Transformationmentioning

confidence: 99%

Factors influencing transient and stable gene expression inSandersonia aurantiacaHook callus tissue

Deroles

Javellana

2011

New Zealand Journal of Crop and Horticultural Science

View full text Add to dashboard Cite

Genetic manipulation of Agrobacterium tumefaciens strains to improve transformation of recalcitrant plant species

Cited by 14 publications

References 55 publications

Field Performance of Five Soybean Mutants Under Drought Stress Conditions and Molecular Analysis Using SSR Markers

Field Performance of Five Soybean Mutants Under Drought Stress Conditions and Molecular Analysis Using SSR Markers

Development of transgenic cabbage (Brassica oleracea var. Capitata) for insect resistance by Agrobacterium tumefaciens-mediated transformation

Factors influencing transient and stable gene expression inSandersonia aurantiacaHook callus tissue

Contact Info

Product

Resources

About