Genetic localization and heterologous expression of validamycin biosynthetic gene cluster isolated from Streptomyces hygroscopicus var. limoneus KCCM 11405 (IFO 12704)

Characterization of the tunicamycin gene cluster unveiling unique steps involved in its biosynthesis

“…Interestingly, the tunL homolog was missing in the potential gene cluster of A. mirum DSM 43827, which may partly account for the lack of tunicamycin production by this strain. In addition, the sequence homologies among the three gene clusters were relatively low (Table S3) compared with the gene clusters of validamycin, which was produced by several Streptomyces, for example (Yu et al, 2005;Bai et al, 2006;Jian et al, 2006;Singh et al, 2006). Remarkably, there are no discernable nucleotide homologies in two tunicamycin gene clusters of S. chartreusis and S. clavuligerus (Table S3).…”

Enhanced validamycin production and gene expression at elevated temperature in Streptomyces hygroscopicus subsp. jingangensis 5008

“…limoneus, and later from S. hygroscopicus var. yingchengensis 10-22 [10][11][12] . In the 45-kb se-ARTICLES MICROBIOLOGY quenced region of S. hygroscopicus 5008, 27 genes were deduced and 8 required structure genes for VAL biosynthesis were defined through heterologous expression (Figure 1(a)).…”

Isolation of the Putative Biosynthetic Gene Cluster of 1-Deoxynojirimycin by <i>Bacillus amyloliquefaciens</i> 140N, Its Production and Application to the Fermentation of Soybean Paste

“…limoneus and acarbose from Actinoplanes sp. 17) However, these actinomycetes are not suitable for use in the food industry. In contrast, Bacillus spp.…”