Genetic linkage maps of rose constructed with new microsatellite markers and locating QTL controlling flowering traits

Abstract

New microsatellites markers [simple sequence repeat (SSR)] have been isolated from rose and integrated into an existing amplified fragment-length polymorphism genetic map. This new map was used to identify quantitative trait locus (QTL) controlling date of flowering and number of petals. From a rose bud expressed sequence tag (EST) database of 2,556 unigenes and a rose genomic library, 44 EST-SSRs and 20 genomic-SSR markers were developed, respectively. These new rose SSRs were used to expand genetic maps of the rose interspecific F 1 progeny. In addition, SSRs from other Rosaceae genera were also tested in the mapping progeny. Genetic maps for the two parents of the progeny were constructed using pseudo-testcross mapping strategy. The maps consist of seven linkage groups of 105 markers covering 432 cM for the maternal map and 136 markers covering 438 cM for the paternal map. Homologous relationships among linkage groups between the maternal and paternal maps were established using SSR markers. Loci controlling flowering traits were localised on genetic maps as a major gene and QTL for the number of petals and a QTL for the blooming date. New SSR markers developed in this study will provide tools for the establishment of a consensus linkage map for roses that combine traits and markers in various rose genetic maps.

Towards a unified genetic map for diploid roses

“…Due to this process, the map length for population 97/7 increased by about 10% whereas the mean marker distance decreased from 1.8 to 1.5 cM. The new calculated integrated map for the HW population had approximately the same length as the published parental maps from Hibrand-Saint Oyant et al (2008) but also a decreased mean marker distance. In comparison to the map from Yan et al (2005), the length of the 94/1 map decreased by 16% to 458 cM while the mean marker distance increased from 1.05 to 1.32 cM because 30% less markers were mapped.…”

“…Populations HW and OBx-WOB26 are connected by 29 bridge markers. To increase the number of markers connecting the populations we tested 27 SSRs from the HW map of Hibrand-Saint Oyant et al (2008) in the populations 94/1 and 97/7. Of these markers 11 could be mapped to the homologous LGs of these two populations.…”

“…The third map (HW) was constructed with AFLP markers by using a diploid population of 91 individuals resulting from an interspeciWc cross between R. wichurana and the dihaploid hybrid H190 (Crespel et al 2002). It was enriched by Hibrand-Saint Oyant et al (2008) and Remay et al (2009) with 45 rose SSRs, 38 rose EST SSRs, 6 Rosaceae EST SSRs and 30 Xowering candidate genes, for both parental maps. In 2005 another mapping population was established based on a R. wichurana cross with 'Basye's Thornless' (Dugo et al 2005).…”

Specific Mapping of Disease Resistance Genes in Tetraploid Cut Roses

“…To this purpose, uni-and bi-parental simplex markers will be mapped by using Joinmap 4.0 (van Ooijen and Voorrips, 2001). Moreover, SSR markers (Süss and Schultze, 2003;Esselink, 2003;Hibrand Saint Oyant et al, 2008) from the literature are currently added to get sufficient anchoring points for proper alignment of rose maps.…”

“…SSRs from the literature which were developed for diploids did not amplify very well on the K5 population. 38% of the SSRs from Hibrand Saint Oyant et al (2008) and 8% of the SSRs from Süss et al (2003) were polymorphic, and used to construct the genetic linkage maps. SSRs from other Rosaceae (strawberry, peach and plum) did not amplify or showed no polymorphism for most of them.…”