Genetic linkage map of 46 DNA markers on human chromosome 16.

Abstract

We have constructed a genetic linkage map of human chromosome 16 based on 46 DNA markers that detect restriction fragment length polymorphisms. Segregation data were collected on a set of multigenerational families provided by the Centre d'Etude du Polymorphisme Humain, and maps were constructed using recently developed multipoint analysis techniques. The map spans 115 centimorgans (cM) in males and 193 cM in females. Over much of the chromosome there is a significantly higher frequency ofrecombination in females than males. Near the a-globin locus on the distal part of the short arm, however, there is a signi t excess of male recombination. Twenty-seven (

Loss of heterozygosity analysis in a human fibrosarcoma cell line

“…The availability of di-, tri-, and tetranuclcotide repeat markers has made it possible to rapidly narrow the regions where multilocus mutational events may have their origin. This is in contrast to studies using RFLP markers, which are both labor-intensive and of limited utility due to the unavailability of easily visual ized, uniformly dispersed, and polymorphic markers of this type along chromosome 16 (Keith et al, 1990;Zhu et al, 1993). For example, our previous studies with MR 12-1 and the 12RD revertant clones derived from it involved PCR amplification of APRT and subsequent analysis of the A vail RFLP to ascertain the presence of one or both APRT alleles (Zhu et al, 1993).…”

Integration of the cytogenetic and genetic linkage maps of human chromosome 16 using 50 physical intervals and 50 polymorphic loci

“…By comparison, our linkage map gives sex-spe cific genetic lengths of 133 cM in males and 202 cM in females, with a sex-averaged length of 165 cM. This compares with esti mates by Keith et al (1990) of 115 cM in males, 193 cM in females, and a sex-averaged distance of 149 cM; estimates by Julier et al (1990) of 187 cM in males and 226 cM in females, and earlier estimates by Donis-Keller et al (1987) of 164 cM in males and 237 cM in females. The distances in this report are in remarkable agreement with the cytogenetic and linkage esti mates of genetic distances reported by Morton (1991).…”

Report of the Second International Workshop on Human Chromosome 16 Mapping 1992

“…Whole chromosome 16 genetic maps have previously been reported (Julier et al, 1990: Keith et al, 1990: Reeders et al, 1991. Many of the markers from these maps, and markers which have subsequently been anchored on the physical map, have been incorporated into the genetic map (Kozman et al,CW 16).…”