Genetic link between β-sarcoglycan and the Egfr signaling pathway

Hashimoto, Reina; Yamaguchi, Masamitsu

doi:10.1016/j.bbrc.2006.07.045

Cited by 9 publications

(10 citation statements)

References 41 publications

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“…It has also been reported that sarcoglycans associate with integrins and nNOS, indicating a potential role in signaling transduction. In fact, we have shown that β-sarcoglycan negatively regulates the Egfr signaling pathway (Hashimoto and Yamaguchi, 2006). These reports suggest that sarcoglycans are multifunctional proteins.…”

Section: Introductionmentioning

confidence: 85%

“…It is interesting to examine the effect of dScgβ knock down on the progression of the cell cycle. However, it has so far proved to be technically difficult to knock down dScgβ in proliferating cells and tissues, most likely because of the relatively long half-life of the dScgβ protein and a high abundance of dScgβ mRNA in these cells (Hashimoto and Yamaguchi, 2006). Establishment and analysis of dScgβ mutant fly lines may help to clarify this point.…”

Section: Sub-cellular Distribution Of Dscgβ During the Cell Cyclementioning

confidence: 99%

“…Rabbit polyclonal anti-dScgβ antibodies were produced as described previously (Hashimoto and Yamaguchi, 2006), and used at 1:4,000 dilution for Western immunoblot analysis, and at 1:200 dilution for immunohistochemistry. Mouse monoclonal anti-α-tubulin and anti-lamin antibodies (Developmental Studies Hybridoma Bank) were both used at 1:400 dilution for immunohistochemistry, and anti-PCNA antibodies (Sigma) were used at 1:2,000 dilution for Western immunoblotting.…”

Section: Antibodiesmentioning

confidence: 99%

“…Canton S was used as the wild type. Transgenic flies carrying Act5C-GAL4>UAS-dscgβ and Act5C-GAL4>UAS-IR-dscgβ were established as described previously (Hashimoto and Yamaguchi, 2006).…”

Section: Fly Stocksmentioning

confidence: 99%

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Dynamic Changes in the Subcellular Localization of Drosophila .BETA.-Sarcoglycan during the Cell Cycle

Hashimoto

Yamaguchi

2006

Cell Struct. Funct.

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ABSTRACT. One of the proposed roles of sarcoglycan is to stabilize dystrophin glycoprotein complexes in muscle sarcolemma. Involvement in signal transduction has also been proposed and abnormalities in some sarcoglycan genes are known to be responsible for muscular dystrophy. While characterization of sarcoglycans in muscle has been performed, little is known about its functions in the non-muscle tissues in which mammalian sarcoglycans are expressed. Here, we investigated temporal and spatial expression patterns of Drosophila β-sarcoglycan (dScgβ) during development by immunohistochemistry. In addition to almost ubiquitous expression in various tissues and organs, as seen for its mammalian counterpart, anti-dScgβ staining data of embryos, eye imaginal discs, and salivary glands demonstrated cytoplasmic localization during S phase in addition to plasma membrane staining. Furthermore we found that subcellular localization of dScgβ dramatically changes during mitosis through possible association with tubulin. These observations point to a complex role of sarcoglycans in non-muscle tissues.

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Section: Introductionmentioning

confidence: 85%

Section: Sub-cellular Distribution Of Dscgβ During the Cell Cyclementioning

confidence: 99%

Section: Antibodiesmentioning

confidence: 99%

“…Canton S was used as the wild type. Transgenic flies carrying Act5C-GAL4>UAS-dscgβ and Act5C-GAL4>UAS-IR-dscgβ were established as described previously (Hashimoto and Yamaguchi, 2006).…”

Section: Fly Stocksmentioning

confidence: 99%

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Dynamic Changes in the Subcellular Localization of Drosophila .BETA.-Sarcoglycan during the Cell Cycle

Hashimoto

Yamaguchi

2006

Cell Struct. Funct.

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“…Western immunoblot analysis was performed as described earlier (Hashimoto and Yamaguchi, 2006) with minor modifications. Blotted membranes were blocked with Tris-buffered saline (50 mM Tris-HCl, pH 8.3 and 150 mM NaCl) containing 10% skim milk for 1 h at 258C and incubated with the anti-dNF-YA antibody at a 1:100 dilution, or an anti-a tubulin monoclonal antibody (Sigma) at a 1:2,000 dilution at 48C for 16 h. The blots were then incubated with a horseradish peroxidase-labeled anti-rabbit IgG (GE Healthcare, Bioscience, Tokyo, Japan) at a 1:2,000 dilution for 1 h at 258C.…”

Section: Western Immunoblot Analysismentioning

confidence: 99%

Complex interference in the eye developmental pathway by Drosophila NF‐YA

Yoshioka

Suyari

Yamada

et al. 2007

Genesis

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The CCAAT motif-binding factor NF-Y consists of three different subunits, NF-YA, NF-YB, and NF-YC, all of which are required for formation of the NF-Y complex and DNA-binding. NF-YA contains a DNA binding domain in its C-terminal region. We established transgenic fly lines carrying the UAS-HA-dNF-YA or UAS-dNF-YAIR and showed over-expression or knockdown with various GAL4 drivers to be lethal at various developmental stages, suggesting that dNF-YA participate in various gene regulatory pathways during Drosophila development. Expression of dNF-YA with eyeless-GAL4 mainly resulted in lethality with a headless phenotype in pharate-adults. Reduction of the eyeless gene dose enhanced the dNF-YA-induced phenotype, while reduction of the Distal-less gene dose suppressed the phenotype. On the other hand, crossing the dNF-YA over-expressing flies with Notch mutant resulted in no apparent effect on the phenotype. These results suggest that dNF-YA can disturb eye disc specification, but not eye disc growth.

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The Roles of the Dystrophin-Associated Glycoprotein Complex at the Synapse

et al. 2009

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Duchenne muscular dystrophy is caused by mutations in the dystrophin gene and is characterized by progressive muscle wasting. A number of Duchenne patients also present with mental retardation. The dystrophin protein is part of the highly conserved dystrophin-associated glycoprotein complex (DGC) which accumulates at the neuromuscular junction (NMJ) and at a variety of synapses in the peripheral and central nervous systems. Many years of research into the roles of the DGC in muscle have revealed its structural function in stabilizing the sarcolemma. In addition, the DGC also acts as a scaffold for various signaling pathways. Here, we discuss recent advances in understanding DGC roles in the nervous system, gained from studies in both vertebrate and invertebrate model systems. From these studies, it has become clear that the DGC is important for the maturation of neurotransmitter receptor complexes and for the regulation of neurotransmitter release at the NMJ and central synapses. Furthermore, roles for the DGC have been established in consolidation of long-term spatial and recognition memory. The challenges ahead include the integration of the behavioral and mechanistic studies and the use of this information to identify therapeutic targets.

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Genetic link between β-sarcoglycan and the Egfr signaling pathway

Cited by 9 publications

References 41 publications

Dynamic Changes in the Subcellular Localization of Drosophila .BETA.-Sarcoglycan during the Cell Cycle

Dynamic Changes in the Subcellular Localization of Drosophila .BETA.-Sarcoglycan during the Cell Cycle

Complex interference in the eye developmental pathway by Drosophila NF‐YA

The Roles of the Dystrophin-Associated Glycoprotein Complex at the Synapse

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