“…For each pool, a target distribution of 600 to 800 nucleotide fragments was collected on a BluePippin instrument (Sage Science Inc., Beverly, MA, USA). Next, PCR was performed to enrich the libraries and to attach external unique TruSeq indices with consequent purification on AMPureXP beads [42]. The quality and concentration of the resulting libraries were checked using a Qubit 2.0 fluorometer (Invitrogen, Carlsbad, CA, USA) and a DNA Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA).…”