Genetic Interaction of Rhizobium Leguminosarum Biovar Viceae with Gram-Negative Bacteria

Döhler, Karl; Klingmüller, Walter

doi:10.1007/978-3-642-73419-9_3

Cited by 13 publications

(6 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a previous study, where E. agglomerans 339 was introduced into unsterile soil microcosms and monitored by plating on selective media, it was shown that the viable counts decreased rapidly from 1 X 10 7 to about 1 × 104 cfu per g of dry soil in 3 weeks [45], which is in agreement with our plating results (Fig. 2).…”

Section: Monitoring Of E Agglomerans 19-1-1 In Long-term Experimentssupporting

confidence: 91%

“…2). From this [45] and also from related experiments [40] it was assumed that E. agglomerans 339 survives poorly in soil. Later it was shown that Tn5 sequences of the E. ag-glomerans 19-1-1 strain could be detected by hybridization of the DNA isolated directly from soil samples up to 70 days after their inoculation.…”

Section: Monitoring Of E Agglomerans 19-1-1 In Long-term Experimentsmentioning

confidence: 91%

See 1 more Smart Citation

Persistence and stability of genetically manipulated derivatives of Enterobacter agglomerans in soil microcosms

Evguenieva‐Hackenberg

1994

FEMS Microbiology Ecology

View full text Add to dashboard Cite

Molecular methods and conventional plating were applied to monitor Enterobacter agglomerans 339 derivatives carrying a Tn5-Mob or an nptI-cassette in unsterile soil microcosms. The plate counts of the introduced bacteria decreased continuously in time until undetectable on selective media. In contrast, hybridization of the total DNA directly isolated from inoculated soil samples showed that the target sequences detected corresponded to a much higher number of bacteria than indicated by plating. By PCR-amplification and hybridization of the soil DNA we could show that a significant number of target sequences still persisted in the soil microcosms, even when the inoculated bacteria were not able to make colonies on selective agar plates. The Tn5 marker caused instabilities in the genome of the bacteria studied. Some of the clones that grew in the soil samples had rearrangements in their genome. The detection of E. agglomerans 339 derivatives carrying the immobile nptI-cassette was also dependent on its location in the bacterial genome. StrainPlasmid (s) Chromosomal marker(s) References E. coli S17-1 pSUP5011 16 E. coli K-12 JM83 pUC4K 33 E. agglomerans 19-1-1 pEA9::Tn5-Mob Rif r, Nal r, Krnr/Nm r. The Kmr/Nm r was 5, 37 due to two copies of Tn5-Mob Rif r, Str r Rif r, Km r, SaC. The Km r and Sac r were due to an nptI-sacB-sacR cassette Rifr Rifr E. agglomerans 339-E. agglomerans 339CR1

show abstract

Section: Monitoring Of E Agglomerans 19-1-1 In Long-term Experimentssupporting

confidence: 91%

Section: Monitoring Of E Agglomerans 19-1-1 In Long-term Experimentsmentioning

confidence: 91%

Persistence and stability of genetically manipulated derivatives of Enterobacter agglomerans in soil microcosms

Evguenieva‐Hackenberg

1994

FEMS Microbiology Ecology

View full text Add to dashboard Cite

show abstract

“…This is in agreement with earlier findings using different systems [2,3,13,20,21], but requires further explanation. This is in agreement with earlier findings using different systems [2,3,13,20,21], but requires further explanation.…”

Section: Discussionsupporting

confidence: 93%

Plasmid transfer in natural soil: a case by case study with nitrogen-fixing Enterobacter

Klingmüller¹

1991

FEMS Microbiology Letters

View full text Add to dashboard Cite

“…R. leguminosarum bv. viciae strains which harbor Tn5 tagged self-transmissible symbiotic plasmids were released in 1987 at a site at Rothamsted Experimental Station, UK [9], Bayreuth University, Germany [19] as well as in 1988 at an experimental plot at INRA, Dijon in France [20]. In none of the studies mentioned, pSym transfer to the resident population or to simultaneously released recipient strains was observed under field conditions.…”

Section: Discussionmentioning

confidence: 99%

Construction and characterization of a Rhizobium leguminosarum biovar viciae strain designed to assess horizontal gene transfer in the environment

Selbitschka¹

1995

FEMS Microbiology Letters

View full text Add to dashboard Cite

An integration vector was developed which inserts cloned DNA in a non-essential site of the Rhizobium leguminosarum biovar viciae chromosome. The expression of integrated genes is under the control of the constitutive neomycin phosphotransferase II (nptII) promoter of transposon Tn5. The design of the vector ensures that loss of vector sequences can be detected, enabling selection of progeny containing only the requisite DNA. The newly constructed vector was employed to insert the Escherichia coli gusA gene conferring GUS activity into R. leguminosarum bv. viciae strain LRS39401 which is cured of its symbiotic plasmid (pSym). One GUS-positive transconjugant, strain CT0370, was shown to have lost all vector sequences. Conjugal transfer of pSym2004 (a Tn5-tagged derivative of symbiotic plasmid pRL1JI, which specifies pea nodulation and symbiotic nitrogen fixation) to CT0370, restored the GUS-positive strain's symbiotic proficiency. Strain CT0370 is presently being used in a field release experiment in order to assess the extent of pSym transfer in a natural R. leguminosarum bv. viciae population under environmental conditions.

show abstract

Genetic Interaction of Rhizobium Leguminosarum Biovar Viceae with Gram-Negative Bacteria

Cited by 13 publications

References 15 publications

Persistence and stability of genetically manipulated derivatives of Enterobacter agglomerans in soil microcosms

Persistence and stability of genetically manipulated derivatives of Enterobacter agglomerans in soil microcosms

Plasmid transfer in natural soil: a case by case study with nitrogen-fixing Enterobacter

Construction and characterization of a Rhizobium leguminosarum biovar viciae strain designed to assess horizontal gene transfer in the environment

Contact Info

Product

Resources

About