Genetic Interaction of Centrosomin and Bazooka in Apical Domain Regulation in Drosophila Photoreceptor

Chen, Geng; Rogers, Alicia K.; League, Garrett P.; Nam, Sang-Chul

doi:10.1371/journal.pone.0016127

Cited by 8 publications

(15 citation statements)

References 44 publications

(77 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3A), which is to be expected as the cellular organelles as well as the acetylated microtubule structures are located outside of the nucleus. The perinuclear localization of Khc is thus consistent with the localization of both Cnn and γ-tubulin, both of which are associated with the microtubule structures in the developing photoreceptor [23]. A similar ubiquitous cytoplasmic distribution of Khc was observed in early eye-discs of third-instar larvae (data now shown).…”

Section: Resultssupporting

confidence: 82%

Role of Kinesin Heavy Chain in Crumbs Localization along the Rhabdomere Elongation in Drosophila Photoreceptor

League

Nam

2011

PLoS ONE

Self Cite

View full text Add to dashboard Cite

BackgroundCrumbs (Crb), a cell polarity gene, has been shown to provide a positional cue for the extension of the apical membrane domain, adherens junction (AJ), and rhabdomere along the growing proximal-distal axis during Drosophila photoreceptor morphogenesis. In developing Drosophila photoreceptors, a stabilized microtubule structure was discovered and its presence was linked to polarity protein localization. It was therefore hypothesized that the microtubules may provide trafficking routes for the polarity proteins during photoreceptor morphogenesis. This study has examined whether Kinesin heavy chain (Khc), a subunit of the microtubule-based motor Kinesin-1, is essential in polarity protein localization in developing photoreceptors.Methodology/Principal FindingsBecause a genetic interaction was found between crb and khc, Crb localization was examined in the developing photoreceptors of khc mutants. khc was dispensable during early eye differentiation and development. However, khc mutant photoreceptors showed a range of abnormalities in the apical membrane domain depending on the position along the proximal-distal axis in pupal photoreceptors. The khc mutant showed a progressive mislocalization in the apical domain along the distal-proximal axis during rhabdomere elongation. The khc mutation also led to a similar progressive defect in the stabilized microtubule structures, strongly suggesting that Khc is essential for microtubule structure and Crb localization during distal to proximal rhabdomere elongation in pupal morphogenesis. This role of Khc in apical domain control was further supported by khc's gain-of-function phenotype. Khc overexpression in photoreceptors caused disruption of the apical membrane domain and the stabilized microtubules in the developing photoreceptors.Conclusions/SignificanceIn summary, we examined the role of khc in the regulation of the apical Crb domain in developing photoreceptors. Since the rhabdomeres in developing pupal eyes grow along the distal-proximal axis, these phenotypes suggest that Khc is essential for the microtubule structures and apical membrane domains during the distal-proximal elongation of photoreceptors, but is dispensable for early eye development.

show abstract

Section: Resultssupporting

confidence: 82%

Role of Kinesin Heavy Chain in Crumbs Localization along the Rhabdomere Elongation in Drosophila Photoreceptor

League

Nam

2011

PLoS ONE

Self Cite

View full text Add to dashboard Cite

show abstract

“…Immunohistochemistry: Fluorescent immunostaining of imaginal discs and pupal eyes were carried out as reported [9][10][11][12][13][14][15]. The following primary antibodies were used: mouse anti-a-Spectrin (Developmental Studies Hybridoma Bank), 1:25; rat anti-Elav (Developmental Studies Hybridoma Bank), 1:25.…”

Section: Geneticsmentioning

confidence: 99%

“…The pupal eye development was utilized for the instructor's main research material for the last 16 years. The instructor has analyzed cell polarity, cell junctions, and cytoskeletons using this important organogenesis model system [9,10,[12][13][14][15][22][23][24][25][26][27].…”

Section: Exercises 8-10: Pupal Eye Developmentmentioning

confidence: 99%

Integration of a faculty's ongoing research into an undergraduate laboratory teaching class in developmental biology

Nam¹

2017

Biochem Molecular Bio Educ

View full text Add to dashboard Cite

Traditional developmental biology laboratory classes have utilized a number of different model organisms to allow students to be exposed to diverse biological phenomena in developing organisms. This traditional approach has mainly focused on the diverse morphological and anatomical descriptions of the developing organisms. However, modern developmental biology is focusing more on conserved genetic networks which are responsible for generating conserved body patterns in developing organisms. Therefore, it is necessary to develop a new pedagogical tool to educate undergraduate biology students in the laboratory class of developmental biology with the genetic principles which are responsible for generating and controlling the developing body patterns. A new undergraduate laboratory class for developmental biology was developed in order to offer students the opportunity to explore a wide range of experimental procedures, also incorporating the instructor's on-going research. Thereby the course can serve as a bridge between research and education by combining both into a single theme. The course design involves a sequence of exercises which can be easily adapted to the faculty's ongoing research. This style of laboratory coursework could be a transitional form between a regular laboratory course and a discovery-based laboratory course. © 2017 by The International Union of Biochemistry and Molecular Biology, 46(2):141-150, 2018.

show abstract

“…The microtubule regulators and microtubule-dependent motors were found to be involved in photoreceptor cell polarity in pupal photoreceptors (Chen et al, 2010(Chen et al, , 2011League and Nam, 2011;Mui et al, 2011;Mukhopadhyay et al, 2010;Nam, 2013Nam, , 2015. Therefore, the Tau might have a role in a microtubule-based trafficking and/or trafficking of cell polarity proteins as a microtubule stabilizer (Doerflinger et al, 2003;Weingarten et al, 1975;Witman et al, 1976) in photoreceptor cell polarity.…”

Section: Mutational Analysis Of Tau In Drosophila Pupal Photoreceptorsmentioning

confidence: 99%

“…Fluorescent immunostaining and confocal analysis of pupal eyes were carried out as reported (Chen et al, 2009(Chen et al, , 2010(Chen et al, , 2011League and Nam, 2011;Choi, 2003, 2006;Nam et al, 2007). Dissected pupal eyes were fixed for 20 min at room temperature in 4% paraformaldehyde.…”

Section: Immunohistochemistrymentioning

confidence: 99%

Role of Tau, a microtubule associated protein, in Drosophila photoreceptor morphogenesis

Nam

2016

Genesis

Self Cite

View full text Add to dashboard Cite

Cell polarity genes have important functions in photoreceptor morphogenesis. Based on recent discovery of stabilized microtubule cytoskeleton in developing photoreceptors and its role in photoreceptor cell polarity, microtubule associated proteins might have important roles in controlling cell polarity proteins' localizations in developing photoreceptors. Here, Tau, a microtubule associated protein, was analyzed to find its potential role in photoreceptor cell polarity. Tau colocalizes with acetylated/stabilized microtubules in developing pupal photoreceptors. Although it is known that tau mutant photoreceptor has no defects in early eye differentiation and development, it shows dramatic disruptions of cell polarity proteins, adherens junctions, and the stable microtubules in developing pupal photoreceptors. This role of Tau in cell polarity proteins' localization in photoreceptor cells during the photoreceptor morphogenesis was further supported by Tau's overexpression studies. Tau overexpression caused dramatic expansions of apical membrane domains where the polarity proteins localize in the developing pupal photoreceptors. It is also found that Tau's role in photoreceptor cell polarity depends on Par-1 kinase. Furthermore, a strong genetic interaction between tau and crumbs was found. It is found that Tau has a crucial role in cell polarity protein localization during pupal photoreceptor morphogenesis stage, but not in early eye development including eye cell differentiation.

show abstract

Genetic Interaction of Centrosomin and Bazooka in Apical Domain Regulation in Drosophila Photoreceptor

Cited by 8 publications

References 44 publications

Role of Kinesin Heavy Chain in Crumbs Localization along the Rhabdomere Elongation in Drosophila Photoreceptor

Role of Kinesin Heavy Chain in Crumbs Localization along the Rhabdomere Elongation in Drosophila Photoreceptor

Integration of a faculty's ongoing research into an undergraduate laboratory teaching class in developmental biology

Role of Tau, a microtubule associated protein, in Drosophila photoreceptor morphogenesis

Contact Info

Product

Resources

About